2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsBrainDendritesDendritic SpinesMiceMicroscopy, ConfocalNeuronsOptics and PhotonicsPatch-Clamp TechniquesStaining and LabelingConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightness
2009
Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes
Homma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Zecevic D. Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes. Philosophical Transactions Of The Royal Society B Biological Sciences 2009, 364: 2453-2467. PMID: 19651647, PMCID: PMC2865128, DOI: 10.1098/rstb.2009.0084.Peer-Reviewed Original ResearchConceptsCalcium-sensitive dyeVoltage-sensitive dyeDendrites of neuronsComplex synaptic inputsDorsal brainstemCell typesBrainstem preparationBath applicationSynaptic inputsRespiratory rhythmSpike activityTwo-photon imagingMammalian brainIon-sensitive dyesIndividual neuronsTwo-photon microscopyPopulation signalsBrainstemBrain activityIndividual cell typesNeuronsBrainMembrane potentialOutput spike trainsSpike trains