2019
Using holographic illumination to study synaptic signal integration at individual dendritic spines
Weng J, Celis C, Zecevic D. Using holographic illumination to study synaptic signal integration at individual dendritic spines. 2019, 10865: 1086514. DOI: 10.1117/12.2507284.Peer-Reviewed Original ResearchSynaptic signal integrationExcitatory synaptic potentialsDendritic spinesSynaptic potentialsTemporal summationAcute cortical brain slicesIndividual synapsesLayer 5 pyramidal neuronsIndividual excitatory synapsesThin basal dendritesCortical brain slicesVoltage-sensitive dye recordingIndividual dendritic spinesSite of originSensory information processingBasal dendritesPyramidal neuronsExcitatory synapsesBrain slicesRepetitive activationVoltage-sensitive dyeSomatic recordingsSingle synapsesSynaptic signalsIndividual neurons
2015
Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. Advances In Experimental Medicine And Biology 2015, 859: 57-101. PMID: 26238049, PMCID: PMC5671121, DOI: 10.1007/978-3-319-17641-3_3.Peer-Reviewed Original ResearchConceptsIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordings
2010
Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Canepari M, Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg B, Grinvald A, Antic S, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. 2010, 25-41. DOI: 10.1007/978-1-4419-6558-5_3.Peer-Reviewed Original ResearchIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordingsSubthreshold eventsSpine
2009
Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes
Homma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Zecevic D. Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes. Philosophical Transactions Of The Royal Society B Biological Sciences 2009, 364: 2453-2467. PMID: 19651647, PMCID: PMC2865128, DOI: 10.1098/rstb.2009.0084.Peer-Reviewed Original ResearchConceptsCalcium-sensitive dyeVoltage-sensitive dyeDendrites of neuronsComplex synaptic inputsDorsal brainstemCell typesBrainstem preparationBath applicationSynaptic inputsRespiratory rhythmSpike activityTwo-photon imagingMammalian brainIon-sensitive dyesIndividual neuronsTwo-photon microscopyPopulation signalsBrainstemBrain activityIndividual cell typesNeuronsBrainMembrane potentialOutput spike trainsSpike trainsWide-Field and Two-Photon Imaging of Brain Activity with Voltage and Calcium-Sensitive Dyes
Homma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Bleau CX, Canepari M, Djurisic M, Zecevic D. Wide-Field and Two-Photon Imaging of Brain Activity with Voltage and Calcium-Sensitive Dyes. Methods In Molecular Biology 2009, 489: 43-79. PMID: 18839087, DOI: 10.1007/978-1-59745-543-5_3.Peer-Reviewed Original ResearchConceptsCalcium-sensitive dyeOlfactory bulb glomeruliCell typesBrain activityIndividual cell typesOlfactory receptor neuronsNerve terminalsSynaptic activityVoltage-sensitive dyeSpike activityTwo-photon imagingVivo preparationMammalian brainReceptor neuronsIndividual neuronsIon-sensitive dyesNeuronsTwo-photon microscopyBrainMembrane potentialLow sensitivitySensitive dyeNoseOutput spike trainsSpike trains
2005
Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes
DJURIŠIĆ M, ZEČEVIĆ D. Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes. Annals Of The New York Academy Of Sciences 2005, 1048: 92-102. PMID: 16154924, DOI: 10.1196/annals.1342.009.Peer-Reviewed Original ResearchConceptsSpike trigger zoneMitral cellsSite of originTrigger zonePrincipal output neuronsTerminal dendritic branchesPrimary dendritesOlfactory nerve stimulationRat olfactory bulbNerve stimulationDendritic tuftsOlfactory bulbVoltage-sensitive dyeAction potentialsNeuronal processesSubthreshold activityIndividual neuronsDendritic branchesSpike initiationIntracellular dyeEPSPsOutput neuronsNeuronsDendritesCells
2004
Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones
Djurisic M, Antic S, Chen WR, Zecevic D. Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones. Journal Of Neuroscience 2004, 24: 6703-6714. PMID: 15282273, PMCID: PMC6729725, DOI: 10.1523/jneurosci.0307-04.2004.Peer-Reviewed Original ResearchConceptsSpike trigger zoneMitral cellsTrigger zoneAction potentialsPrincipal output neuronsTerminal dendritic branchesPrimary dendritesOlfactory nerve stimulationRat olfactory bulbSite of originNerve stimulationDendritic tuftsSecondary dendritesEPSP attenuationOlfactory bulbSynaptic sitesNeuronal processesIndividual neuronsDendritic branchesSpike initiationIntracellular dyeEPSPsOutput neuronsNeuronsDendrites
2000
Functional profile of the giant metacerebral neuron of Helix aspersa: temporal and spatial dynamics of electrical activity in situ
Antic S, Wuskell J, Loew L, Zecevic D. Functional profile of the giant metacerebral neuron of Helix aspersa: temporal and spatial dynamics of electrical activity in situ. The Journal Of Physiology 2000, 527: 55-69. PMID: 10944170, PMCID: PMC2270048, DOI: 10.1111/j.1469-7793.2000.00055.x.Peer-Reviewed Original ResearchConceptsTrigger zoneNeuronal processesGiant metacerebral neuronSingle neuronsMulti-site optical recordingSpike trigger zoneAction potential initiationIdentified snail neuronsMetacerebral neuronsAxonal branchesVoltage-sensitive dyeSynaptic inputsPropagation of spikesSnail neuronsAction potentialsAxonal spikesPatterns of initiationNeuronsPotential initiationSynaptic signalsIndividual neuronsElectrical activityHelix aspersaFunctional profilesInitiation
1999
Chapter sixteen Imaging Membrane Potential Changes in Individual Neurons
Antić S, Zečević D. Chapter sixteen Imaging Membrane Potential Changes in Individual Neurons. 1999, 238-248. DOI: 10.1016/b978-012447836-7/50018-x.Peer-Reviewed Original ResearchVoltage-sensitive dyeIndividual neuronsSingle neuronsVoltage-sensitive dye recordingSpatial resolutionIndirect optical measurementsDirect electrical recordingOptical signalGood spatial resolutionOptical measurementsIndividual nerve cellsPresent sensitivityFunctional neuronal networksNeuronal dendritic treeOptical recordingDendritic treeNerve cellsDye injectionNeuronsIntracellular applicationMembrane potential changesNeuronal networksMultisite recordingsElectrical recordingsLocal modulation
1995
Optical signals from neurons with internally applied voltage-sensitive dyes
Antic S, Zecevic D. Optical signals from neurons with internally applied voltage-sensitive dyes. Journal Of Neuroscience 1995, 15: 1392-1405. PMID: 7869106, PMCID: PMC6577832, DOI: 10.1523/jneurosci.15-02-01392.1995.Peer-Reviewed Original ResearchConceptsOptical signalVoltage-sensitive dyeSilicon photodiode arrayAxonal branchesAction potentialsIndividual neuronsOptical monitoringPresent sensitivityPhotodiode arraySite of stimulationAction potential initiationLong axonal branchesSatisfactory signalIntracellular pressure injectionSynaptic potentialsFluorescence measurementsGood signalAxonal segmentsSubthreshold potentialsExtracellular applicationCell bodiesNeuronal processesFluorescence signalElectrotonic responsesPotential initiation
1989
17 Optical Measurement of Membrane Potential in Invertebrate Ganglia and Mammalian Cortex
WU J, LONDON J, ZECEVIC D, LOEW L, ORBACH H, CATTARELLI M, COHEN L. 17 Optical Measurement of Membrane Potential in Invertebrate Ganglia and Mammalian Cortex. 1989, 329-346. DOI: 10.1016/b978-0-12-417760-4.50024-0.Peer-Reviewed Original Research