2019
Using holographic illumination to study synaptic signal integration at individual dendritic spines
Weng J, Celis C, Zecevic D. Using holographic illumination to study synaptic signal integration at individual dendritic spines. 2019, 10865: 1086514. DOI: 10.1117/12.2507284.Peer-Reviewed Original ResearchSynaptic signal integrationExcitatory synaptic potentialsDendritic spinesSynaptic potentialsTemporal summationAcute cortical brain slicesIndividual synapsesLayer 5 pyramidal neuronsIndividual excitatory synapsesThin basal dendritesCortical brain slicesVoltage-sensitive dye recordingIndividual dendritic spinesSite of originSensory information processingBasal dendritesPyramidal neuronsExcitatory synapsesBrain slicesRepetitive activationVoltage-sensitive dyeSomatic recordingsSingle synapsesSynaptic signalsIndividual neurons
2015
Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. Advances In Experimental Medicine And Biology 2015, 859: 57-101. PMID: 26238049, PMCID: PMC5671121, DOI: 10.1007/978-3-319-17641-3_3.Peer-Reviewed Original ResearchConceptsIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordings
2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightness
2010
Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Canepari M, Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg B, Grinvald A, Antic S, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. 2010, 25-41. DOI: 10.1007/978-1-4419-6558-5_3.Peer-Reviewed Original ResearchIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordingsSubthreshold eventsSpineRapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons
Holthoff K, Zecevic D, Konnerth A. Rapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons. The Journal Of Physiology 2010, 588: 1085-1096. PMID: 20156851, PMCID: PMC2852997, DOI: 10.1113/jphysiol.2009.184960.Peer-Reviewed Original ResearchConceptsRapid time courseAction potentialsDendritic spinesIndividual dendritic spinesLayer 5 pyramidal neuronsTime courseTiming-dependent synaptic plasticityDendritic branchesCentral mammalian neuronsTerminal dendritic branchesVisual cortex neuronsTerminal branchesSomatic action potentialsIndividual spinesRemote dendritesPyramidal neuronsApical dendritesCortex neuronsExcitatory synapsesSupralinear integrationSynaptic inputsVoltage-sensitive dyeJuvenile miceSynaptic plasticityVisual cortex