Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes
Homma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Zecevic D. Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes. Philosophical Transactions Of The Royal Society B Biological Sciences 2009, 364: 2453-2467. PMID: 19651647, PMCID: PMC2865128, DOI: 10.1098/rstb.2009.0084.Peer-Reviewed Original ResearchConceptsCalcium-sensitive dyeVoltage-sensitive dyeDendrites of neuronsComplex synaptic inputsDorsal brainstemCell typesBrainstem preparationBath applicationSynaptic inputsRespiratory rhythmSpike activityTwo-photon imagingMammalian brainIon-sensitive dyesIndividual neuronsTwo-photon microscopyPopulation signalsBrainstemBrain activityIndividual cell typesNeuronsBrainMembrane potentialOutput spike trainsSpike trainsWide-Field and Two-Photon Imaging of Brain Activity with Voltage and Calcium-Sensitive Dyes
Homma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Bleau CX, Canepari M, Djurisic M, Zecevic D. Wide-Field and Two-Photon Imaging of Brain Activity with Voltage and Calcium-Sensitive Dyes. Methods In Molecular Biology 2009, 489: 43-79. PMID: 18839087, DOI: 10.1007/978-1-59745-543-5_3.Peer-Reviewed Original ResearchConceptsCalcium-sensitive dyeOlfactory bulb glomeruliCell typesBrain activityIndividual cell typesOlfactory receptor neuronsNerve terminalsSynaptic activityVoltage-sensitive dyeSpike activityTwo-photon imagingVivo preparationMammalian brainReceptor neuronsIndividual neuronsIon-sensitive dyesNeuronsTwo-photon microscopyBrainMembrane potentialLow sensitivitySensitive dyeNoseOutput spike trainsSpike trains