2024
Comparison of test performance of a conventional PCR and two field-friendly tests to detect Coxiella burnetii DNA in ticks using Bayesian latent class analysis
Kamau M, Witte C, Goosen W, Mutinda M, Villinger J, Getange D, Khogali R, von Fricken M, Fèvre E, Zimmerman D, Linton Y, Miller M. Comparison of test performance of a conventional PCR and two field-friendly tests to detect Coxiella burnetii DNA in ticks using Bayesian latent class analysis. Frontiers In Veterinary Science 2024, 11: 1396714. PMID: 38962707, PMCID: PMC11220323, DOI: 10.3389/fvets.2024.1396714.Peer-Reviewed Original ResearchBayesian latent class analysisC. burnetii DNAEpidemiological roleHuman Q fever outbreakBayesian latent class analysis modelWild animalsDetect Coxiella burnetii DNADetect C. burnetii DNACoxiella burnetii DNAWild animal samplesPCR-HRMQ fever outbreakField-friendly techniquePositive ticksPCR high-resolution meltingTicksPCR-HRM assayZoonotic threatAnimal samplesWild animal habitatsQ feverHigh-resolution meltingMolecular techniquesCPCREstimate SE
2022
Metagenomic Investigation of Ticks From Kenyan Wildlife Reveals Diverse Microbial Pathogens and New Country Pathogen Records
Ergunay K, Mutinda M, Bourke B, Justi SA, Caicedo-Quiroga L, Kamau J, Mutura S, Akunda IK, Cook E, Gakuya F, Omondi P, Murray S, Zimmerman D, Linton YM. Metagenomic Investigation of Ticks From Kenyan Wildlife Reveals Diverse Microbial Pathogens and New Country Pathogen Records. Frontiers In Microbiology 2022, 13: 932224. PMID: 35847110, PMCID: PMC9283121, DOI: 10.3389/fmicb.2022.932224.Peer-Reviewed Original ResearchDiverse microbial pathogensPathogenic bacterial speciesHuman mastadenovirus CVertebrate taxaPhylogenetic reconstructionMajor phylaFungal speciesMetagenomic investigationWild ungulatesMicrobial pathogensBacterial speciesWild animalsRelative abundanceSignificant divergenceHuman pathogenicitySpeciesPathogen transferPathogensBovine herpesvirus 5Filarial pathogensVirus 1First reportKenyan drylandsPoolTicks