2005
Expression of activation-induced cytidine deaminase is regulated by cell division, providing a mechanistic basis for division-linked class switch recombination
Rush JS, Liu M, Odegard VH, Unniraman S, Schatz DG. Expression of activation-induced cytidine deaminase is regulated by cell division, providing a mechanistic basis for division-linked class switch recombination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13242-13247. PMID: 16141332, PMCID: PMC1201576, DOI: 10.1073/pnas.0502779102.Peer-Reviewed Original ResearchConceptsClass switch recombinationCell divisionAID expressionSwitch recombinationFrequency of CSRSingle cell divisionSubsequent cell divisionSuccessive cell divisionsActivation-induced cytidine deaminaseConstitutive AID expressionIg heavy chain constant regionsEffector function propertiesHeavy chain constant regionActivation-induced cytidine deaminase mRNAMolecular explanationMechanistic basisDifferent molecular featuresSuccessive divisionsChain constant regionCytidine deaminaseB cell activationCytokine exposureExpressionConstant regionCell activation
2004
Staggered AID‐dependent DNA double strand breaks are the predominant DNA lesions targeted to Sµ in Ig class switch recombination
Rush JS, Fugmann SD, Schatz DG. Staggered AID‐dependent DNA double strand breaks are the predominant DNA lesions targeted to Sµ in Ig class switch recombination. International Immunology 2004, 16: 549-557. PMID: 15039385, DOI: 10.1093/intimm/dxh057.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalBlotting, SouthernB-LymphocytesCell DivisionCytidine DeaminaseDeoxyribonucleases, Type II Site-SpecificDNADNA DamageDNA PrimersFlow CytometryGene ExpressionImmunoglobulin Class SwitchingImmunoglobulin DImmunoglobulin GImmunoglobulin Switch RegionInterleukin-4LipopolysaccharidesMiceMice, Inbred C57BLMice, KnockoutPlasmidsPolymerase Chain ReactionRecombination, GeneticConceptsClass switch recombinationDNA double-strand breaksPredominant DNA lesionsDouble-strand breaksActivation-induced cytidine deaminaseDNA lesionsSwitch recombinationAID-dependent DNA double-strand breaksStrand breaksIg class switch recombinationLigation-mediated PCRS mu regionCellular regulationKinetics of inductionMolecular detailsMurine B cellsDNA DSBsStaggered breaksCytidine deaminaseDSBsMu regionMinor speciesB cellsS muEffector properties
1999
Distinct effects of Jak3 signaling on alphabeta and gammadelta thymocyte development.
Eynon E, Livák F, Kuida K, Schatz D, Flavell R. Distinct effects of Jak3 signaling on alphabeta and gammadelta thymocyte development. The Journal Of Immunology 1999, 162: 1448-59. PMID: 9973401, DOI: 10.4049/jimmunol.162.3.1448.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell DivisionCell SurvivalGene ExpressionGene Rearrangement, delta-Chain T-Cell Antigen ReceptorGene Rearrangement, gamma-Chain T-Cell Antigen ReceptorGenes, bcl-2Janus Kinase 3MiceMice, Inbred C57BLMice, KnockoutMice, TransgenicProtein-Tyrosine KinasesReceptors, Antigen, T-Cell, alpha-betaReceptors, Antigen, T-Cell, gamma-deltaSignal TransductionT-Lymphocyte SubsetsConceptsJak3-/- miceGammadelta T cell lineagesThymocyte developmentTransduction of signalsTCRbeta chain gene rearrangementLineage differentiationGammadelta lineageCell lineagesGene resultsKinase 3Developmental blockadeEarly thymocyte differentiationCytokine receptorsGamma locusT-cell lineageTargeted deletionBcl-2 expressionThymocyte differentiationTCRbeta transgeneIL-2 familyLineagesDifferentiationImmature thymocytesTransgeneSevere reduction
1993
Multiple rearrangements in T cell receptor alpha chain genes maximize the production of useful thymocytes.
Petrie HT, Livak F, Schatz DG, Strasser A, Crispe IN, Shortman K. Multiple rearrangements in T cell receptor alpha chain genes maximize the production of useful thymocytes. Journal Of Experimental Medicine 1993, 178: 615-622. PMID: 8393478, PMCID: PMC2191132, DOI: 10.1084/jem.178.2.615.Peer-Reviewed Original ResearchConceptsAlpha/beta heterodimerMultiple rearrangementsTCR alpha/beta heterodimerBeta heterodimerBeta chainT-cell receptor alpha-chain geneChain geneTCR alpha geneTCR alphaSurface expressionReceptor alpha-chain geneTransgenic TCR beta chainTCR alpha locusAlpha chain geneT cell receptor alphaAlpha gene rearrangementsMajority of TCREndogenous TCR alpha chainsHeavy chain genePositive selectionSomatic rearrangementsAllelic exclusionAlpha geneAlpha locusTCR alpha chain
1991
The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system
Chun J, Schatz D, Oettinger M, Jaenisch R, Baltimore D. The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system. Cell 1991, 64: 189-200. PMID: 1986864, DOI: 10.1016/0092-8674(91)90220-s.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsBase SequenceBrainCell DivisionCell LineChromosome MappingCytarabineGenesMiceMice, Inbred BALB CMolecular Sequence DataNeuronsNucleic Acid HybridizationOligonucleotide ProbesOrgan SpecificityPolymerase Chain ReactionRecombination, GeneticRNA ProbesTeratomaTranscription, GeneticTretinoinConceptsCentral nervous systemRAG-1 transcriptsMurine central nervous systemNervous systemHigh neuronal cell densityRAG-2 transcriptsSite-specific recombination machineryNeuronal cell densityNorthern blot analysisRecombination machineryGenome sitesPolymerase chain reactionRAG-2Postnatal brainPostnatal neuronsDetrimental alterationsRAG-1TranscriptsSitu hybridizationBlot analysisChain reactionNeuronsCell densityRecombination