2018
Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting
Murray C, Miwa H, Dhar M, Park DE, Pao E, Martinez J, Kaanumale S, Loghin E, Graf J, Rhaddassi K, Kwok WW, Hafler D, Puleo C, Di Carlo D. Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting. Lab On A Chip 2018, 18: 2396-2409. PMID: 30039125, PMCID: PMC6095198, DOI: 10.1039/c8lc00518d.Peer-Reviewed Original Research
2011
The CD6 Multiple Sclerosis Susceptibility Allele Is Associated with Alterations in CD4+ T Cell Proliferation
Kofler DM, Severson CA, Mousissian N, De Jager PL, Hafler DA. The CD6 Multiple Sclerosis Susceptibility Allele Is Associated with Alterations in CD4+ T Cell Proliferation. The Journal Of Immunology 2011, 187: 3286-3291. PMID: 21849685, DOI: 10.4049/jimmunol.1100626.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAntigens, CDAntigens, Differentiation, T-LymphocyteCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell ProliferationCell SeparationCells, CulturedFemaleFlow CytometryGenetic Predisposition to DiseaseGenotypeHumansMaleMultiple SclerosisPhenotypeReverse Transcriptase Polymerase Chain ReactionRisk FactorsRNA, Small InterferingConceptsGenome-wide association studiesAssociation studiesAllelic variantsNew susceptibility lociSusceptibility allelesRisk allelesProliferation defectExon 5Risk-associated allelesSingle nucleotide polymorphismsExtracellular binding sitesCD6 geneSusceptibility lociLinkage disequilibriumMS risk alleleSelective knockdownT cell activationNucleotide polymorphismsAltered proliferationCell proliferationGenetic associationAllelesLong-term activationBinding sitesMS susceptibility alleles
2010
Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine
Konry T, Dominguez-Villar M, Baecher-Allan C, Hafler DA, Yarmush ML. Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine. Biosensors And Bioelectronics 2010, 26: 2707-2710. PMID: 20888750, PMCID: PMC3141325, DOI: 10.1016/j.bios.2010.09.006.Peer-Reviewed Original Research
2006
Human regulatory T cells and their role in autoimmune disease
Baecher‐Allan C, Hafler DA. Human regulatory T cells and their role in autoimmune disease. Immunological Reviews 2006, 212: 203-216. PMID: 16903916, DOI: 10.1111/j.0105-2896.2006.00417.x.Peer-Reviewed Original ResearchConceptsRegulatory T cellsAutoimmune diseasesSelf-reactive cellsT cellsHealthy individualsHuman natural regulatory T cellsSelf-reactive T cellsNatural regulatory T cellsHuman regulatory T cellsPathologic T cellsReduced inhibitory functionSpecific autoimmune diseasesT cell populationsMature T cellsT cell maturationNon-responsive stateRegulatory cellsNatural CD4Peripheral circulationImmune systemPatientsDiseaseInhibitory functionNumber of mechanismsCells
2004
Human CD4+CD25+ regulatory T cells
Baecher-Allan C, Viglietta V, Hafler DA. Human CD4+CD25+ regulatory T cells. Seminars In Immunology 2004, 16: 89-98. PMID: 15036232, DOI: 10.1016/j.smim.2003.12.005.Peer-Reviewed Original ResearchConceptsRegulatory T cellsT reg cellsT cellsT reg populationT cell subsetsT-reg functionHuman peripheral bloodIL-10Lymph nodesPeripheral bloodCell subsetsFunctional outcomeCord bloodTCR stimuliRapid effectsCellular compositionTCR signalsPotential involvementActivation stateStrong stimulationMiceBloodDisparate findingsCellsCulture conditions
2003
Activated CD8+ T cells in secondary progressive MS secrete lymphotoxin
Buckle GJ, Höllsberg P, Hafler DA. Activated CD8+ T cells in secondary progressive MS secrete lymphotoxin. Neurology 2003, 60: 702-705. PMID: 12601116, DOI: 10.1212/01.wnl.0000048204.89346.30.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntibodiesCD3 ComplexCD8-Positive T-LymphocytesCell DivisionCell SeparationCells, CulturedCytokinesFemaleFlow CytometryGene FrequencyHumansLymphotoxin-alphaMaleMiddle AgedMultiple Sclerosis, Chronic ProgressiveMultiple Sclerosis, Relapsing-RemittingPolymorphism, Single NucleotideReference ValuesConceptsT cellsNormal controlsSecondary progressive MSCytokine secretion profileFunctional activation statesLymphotoxin secretionProgressive MSActivated CD8Cytokine secretionSecretion profileCytokine genesCD8SecretionSignificant differencesPatientsSignificant increaseActivation stateSingle nucleotide polymorphism analysisPolymorphism analysisNucleotide polymorphism analysisCells
2000
Human and Murine CD4 T Cell Reactivity to a Complex Antigen: Recognition of the Synthetic Random Polypeptide Glatiramer Acetate
Duda P, Krieger J, Schmied M, Balentine C, Hafler D. Human and Murine CD4 T Cell Reactivity to a Complex Antigen: Recognition of the Synthetic Random Polypeptide Glatiramer Acetate. The Journal Of Immunology 2000, 165: 7300-7307. PMID: 11120865, DOI: 10.4049/jimmunol.165.12.7300.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsCD4-Positive T-LymphocytesCell Line, TransformedCell SeparationClone CellsDose-Response Relationship, ImmunologicFemaleGlatiramer AcetateHematopoietic Stem CellsHLA-DR AntigensHumansImmunizationImmunologic MemoryImmunomagnetic SeparationInfant, NewbornLeukocytes, MononuclearLymphocyte ActivationLymphocyte CountMiceMice, Inbred BALB CMice, Inbred C57BLMultiple Sclerosis, Relapsing-RemittingPeptidesSpleenTh1 CellsTh2 CellsConceptsT cell populationsHLA class II DRGlatiramer acetateT cell proliferationClass II DRII DRT cellsCD4 T cell reactivityGA-reactive T cellsHuman T cell proliferative responsesT cell precursor frequencyCell populationsSpecific human T cell clonesT cell proliferative responsesHuman T cell clonesMemory T cellsT cell reactivityMultiple sclerosis patientsRecent clinical findingsCell precursor frequencyCell proliferative responsesCell proliferationT cell clonesDose-dependent proliferationHealthy human adultsA novel population of B7‐1+ T cells producing intracellular IL‐4 is decreased in patients with multiple sclerosis
Kipp B, Bar‐Or A, Gausling R, Oliveira E, Fruhan S, Stuart W, Hafler D. A novel population of B7‐1+ T cells producing intracellular IL‐4 is decreased in patients with multiple sclerosis. European Journal Of Immunology 2000, 30: 2092-2100. PMID: 10940899, DOI: 10.1002/1521-4141(200007)30:7<2092::aid-immu2092>3.0.co;2-7.Peer-Reviewed Original ResearchConceptsT cell receptorIntracellular IL-4Multiple sclerosisT cellsB7-1IL-4Autoimmune diseasesTNF-alphaIFN-gammaIL-4-producing T cellsLittle IL-4Immunoregulatory T cellsIL-4 productionIntracellular IFN-gammaT cell populationsLittle IFN-gammaNovel populationDiverse TCR repertoireMHC class IIHuman T cellsShort-term cultureCell surface moleculesTCR repertoireNormal subjectsPatients
1986
Antigen reactive memory T cells are defined by Ta1.
Hafler DA, Fox DA, Benjamin D, Weiner HL. Antigen reactive memory T cells are defined by Ta1. The Journal Of Immunology 1986, 137: 414-8. PMID: 3013990, DOI: 10.4049/jimmunol.137.2.414.Peer-Reviewed Original ResearchConceptsT cellsReactive memory T cellsPeripheral blood T cellsAutologous mixed lymphocyte responseHuman peripheral blood T cellsMemory T cellsMixed lymphocyte responseBlood T cellsPresence of PWMT cell clonesEnhanced proliferative responseTA1 cellsActivated T cellsNormal human subjectsT cell linesT memory cellsSuppressor cellsLymphocyte responsesAnamnestic responseMitogen PHAPeripheral bloodLymphocyte populationsIL-2Tetanus toxoidT lymphocytes