A Small-Scale shRNA Screen in Primary Mouse Macrophages Identifies a Role for the Rab GTPase Rab1b in Controlling Salmonella Typhi Growth
Solano-Collado V, Colamarino RA, Calderwood DA, Baldassarre M, Spanò S. A Small-Scale shRNA Screen in Primary Mouse Macrophages Identifies a Role for the Rab GTPase Rab1b in Controlling Salmonella Typhi Growth. Frontiers In Cellular And Infection Microbiology 2021, 11: 660689. PMID: 33898333, PMCID: PMC8059790, DOI: 10.3389/fcimb.2021.660689.Peer-Reviewed Original ResearchConceptsShRNA screenMouse macrophagesGenome-wide screeningRab GTPasesRab GTPasePrimary mouse macrophagesGTPase Rab1bNext-generation sequencingLife-threatening systemic infectionsUnbiased identificationFluorescent populationsConcept screenIntracellular pathogensPrimary immune cellsRab32Bacterial pathogensRab1bHuman hostPrimary mouseInfected cellsHuman macrophagesImportance of macrophagesSalmonella typhi growthTyphi infectionImmune cells