2023
Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis
Huet-Calderwood C, Rivera-Molina F, Toomre D, Calderwood D. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis. Methods In Molecular Biology 2023, 2608: 17-38. PMID: 36653699, PMCID: PMC9999384, DOI: 10.1007/978-1-0716-2887-4_2.ChaptersConceptsΒ1 integrinTotal internal reflection fluorescence microscopyNormal cell adhesionIntegrin adhesion receptorsReflection fluorescence microscopyAdhesion receptorsCell adhesionEndocytosisFluorescence microscopyExocytosisIntegrinsCellsHaloTagPHluorinIntracellular labelingEctoPhotobleachingTagsReceptorsChaseFluorescentAdhesionLabelingMigration
2022
Organization, dynamics and mechanoregulation of integrin-mediated cell–ECM adhesions
Kanchanawong P, Calderwood DA. Organization, dynamics and mechanoregulation of integrin-mediated cell–ECM adhesions. Nature Reviews Molecular Cell Biology 2022, 24: 142-161. PMID: 36168065, PMCID: PMC9892292, DOI: 10.1038/s41580-022-00531-5.Peer-Reviewed Original ResearchConceptsExtracellular matrixCell-ECM adhesionCell-ECM interactionsLocal extracellular matrixAdhesion maturationAdhesion complexesAnimal cellsBiochemical signalingTransmembrane receptorsAdhesion structuresCell shapeIntegrin familyMolecular natureAge-related dysfunctionAdvanced imaging approachesCharacterization of rearrangementsMechanical forcesSignalingTissue formationAdhesionCytoskeletonMechanoregulationImmune responseImaging approachImproved understanding
2020
Differences in self-association between kindlin-2 and kindlin-3 are associated with differential integrin binding
Kadry YA, Maisuria EM, Huet-Calderwood C, Calderwood DA. Differences in self-association between kindlin-2 and kindlin-3 are associated with differential integrin binding. Journal Of Biological Chemistry 2020, 295: 11161-11173. PMID: 32546480, PMCID: PMC7415974, DOI: 10.1074/jbc.ra120.013618.Peer-Reviewed Original ResearchConceptsKindlin-3Kindlin-2Focal adhesionsIntegrin cytoplasmic domainTransmembrane adhesion receptorsComparative sequence analysisLive-cell imagingAbility of cellsCytoplasmic domainF3 subdomainsMammalian cellsCytoplasmic componentsExtracellular environmentAdhesion receptorsKindlinSequence analysisIntegrin familySelf-associationIntegrin bindingPhysiological importanceMolecular levelPoint mutationsProteinCellsAdhesion
2019
Filamin A mediates isotropic distribution of applied force across the actin network
Kumar A, Shutova MS, Tanaka K, Iwamoto DV, Calderwood DA, Svitkina TM, Schwartz MA. Filamin A mediates isotropic distribution of applied force across the actin network. Journal Of Cell Biology 2019, 218: 2481-2491. PMID: 31315944, PMCID: PMC6683746, DOI: 10.1083/jcb.201901086.Peer-Reviewed Original ResearchConceptsTalin tension sensorStress fibersActin networkFilamin ACortical actin networkCortical actin filamentsIntegrin-mediated adhesionActin cytoskeletonFocal adhesionsCortical actinFLNA knockdownActin filamentsTalinKnockdownCell sensingDirection of stretchTension sensorPhysiology of muscleUniaxial stretchForce transmissionCytoskeletonStrainsStretchAdhesionReexpression
2017
Novel ecto-tagged integrins reveal their trafficking in live cells
Huet-Calderwood C, Rivera-Molina F, Iwamoto DV, Kromann EB, Toomre D, Calderwood DA. Novel ecto-tagged integrins reveal their trafficking in live cells. Nature Communications 2017, 8: 570. PMID: 28924207, PMCID: PMC5603536, DOI: 10.1038/s41467-017-00646-w.Peer-Reviewed Original ResearchConceptsIntegrin functionΒ1 integrinLive cellsCell surface adhesion receptorsHeterodimeric cell-surface adhesion receptorsIntegrin endocytosisMulticellular organismsNovel powerful toolFocal adhesionsKnockout fibroblastsIntegrin activationAdhesion receptorsExtracellular loopIntegrinsTraffickingMajor mysteriesCellsTagsAdhesionHaloTagEndocytosisPowerful toolExocytosisOrganismsVesicles
2015
Regulation of integrin-mediated adhesions
Iwamoto DV, Calderwood DA. Regulation of integrin-mediated adhesions. Current Opinion In Cell Biology 2015, 36: 41-47. PMID: 26189062, PMCID: PMC4639423, DOI: 10.1016/j.ceb.2015.06.009.Peer-Reviewed Original ResearchConceptsIntegrin-mediated adhesionHeterodimeric transmembrane adhesion receptorsShort cytoplasmic tailTransmembrane adhesion receptorsSpecific intracellular proteinsClustering of integrinsMetazoan developmentActin cytoskeletonExtracellular ligandsCytoplasmic tailIntracellular traffickingExtracellular environmentIntracellular proteinsAdhesion receptorsAdhesive structuresIntegrin receptorsCell membraneRelay signalsIntegrinsEssential roleMechanical forcesCell attachmentAdhesionRecent advancesCytoskeletonPAK6 targets to cell–cell adhesions through its N-terminus in a Cdc42-dependent manner to drive epithelial colony escape
Morse EM, Sun X, Olberding JR, Ha BH, Boggon TJ, Calderwood DA. PAK6 targets to cell–cell adhesions through its N-terminus in a Cdc42-dependent manner to drive epithelial colony escape. Journal Of Cell Science 2015, 129: 380-393. PMID: 26598554, PMCID: PMC4732285, DOI: 10.1242/jcs.177493.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, CDCadherinsCdc42 GTP-Binding ProteinCell AdhesionCell Line, TumorEpithelial CellsHEK293 CellsHumansIntercellular JunctionsMolecular Sequence DataP21-Activated KinasesProtein BindingProtein Interaction Domains and MotifsProtein Sorting SignalsProtein TransportConceptsCell-cell adhesionN-terminusCdc42/Rac interactive binding (CRIB) domainSerine/threonine kinaseP21-activated kinase (PAK) familyCdc42-dependent mannerPolybasic regionThreonine kinaseCdc42 knockdownKinase familyBinding domainsKinase activityImportant regulatorCell adhesionPAK6Broader rolePAKAdhesionTargetingCdc42PAK1KinaseKnockdownRegulatorMutations
2012
Functional differences between kindlin-1 and kindlin-2 in keratinocytes
Bandyopadhyay A, Rothschild G, Kim S, Calderwood DA, Raghavan S. Functional differences between kindlin-1 and kindlin-2 in keratinocytes. Journal Of Cell Science 2012, 125: 2172-2184. PMID: 22328497, PMCID: PMC3367939, DOI: 10.1242/jcs.096214.Peer-Reviewed Original ResearchConceptsFocal adhesionsKindlin-2Kindlin-1Cell spreadingPeripheral focal adhesionsIntegrin β1Wild-type cellsUnexpected functional consequencesIntegrin β6Wild-type keratinocytesCytoplasmic tailNull keratinocytesKindlinNull cellsFunctional consequencesDirect interactionFunctional differencesUnique functionRelated integrinsIntegrinsCellsAdhesionKeratinocytesIntegrin αvβ6Knockdown
2008
JAM-L–mediated leukocyte adhesion to endothelial cells is regulated in cis by α4β1 integrin activation
Luissint AC, Lutz PG, Calderwood DA, Couraud PO, Bourdoulous S. JAM-L–mediated leukocyte adhesion to endothelial cells is regulated in cis by α4β1 integrin activation. Journal Of Cell Biology 2008, 183: 1159-1173. PMID: 19064666, PMCID: PMC2600739, DOI: 10.1083/jcb.200805061.Peer-Reviewed Original ResearchConceptsJunctional adhesion moleculeIntegrin activationIntegrin-dependent adhesionIntegrin VLA-4Endothelial cellsAdhesion moleculesEpithelial adhesion moleculesJAM familyHeterophilic interactionsCis dimerizationDimerization stateVLA-4Monomeric formLeukocyte integrin VLA-4CellsActivationAdhesionComplexesProteinMoleculesDimerizationLeukocyte adhesionRecruitmentAccumulationFamily
2007
Forces and Bond Dynamics in Cell Adhesion
Evans EA, Calderwood DA. Forces and Bond Dynamics in Cell Adhesion. Science 2007, 316: 1148-1153. PMID: 17525329, DOI: 10.1126/science.1137592.Peer-Reviewed Original ResearchConceptsSingle-molecule force spectroscopyCell adhesionDissociation of bondsMolecular cell biologyCell signaling processesBond dynamicsForce spectroscopySurface bondingCell biologySignaling processesAdhesion receptorsLarge moleculesCell biochemistryChemical processesMolecular interactionsExtracellular matrixBondsIntracellular sitesAdhesion bondsChemical circuitryCellsBiological cellsKey nodesAdhesionSpectroscopyIntegrin Cytoskeletal Interactions
Lad Y, Harburger DS, Calderwood DA. Integrin Cytoskeletal Interactions. Methods In Enzymology 2007, 426: 69-84. PMID: 17697880, DOI: 10.1016/s0076-6879(07)26004-5.Peer-Reviewed Original ResearchConceptsIntegrin cytoplasmic tailsCytoplasmic tailProtein-protein interaction studiesIntegrin-binding proteinsIntegrin adhesion receptorsCell-substratum adhesionCytoskeletal interactionsPlasma membraneCytoskeletal proteinsBiochemical signalsAdhesion receptorsIntracellular ligandsTail interactionsCellular activitiesIntegrin-cytoskeletal interactionsMechanical forcesRecombinant modelProteinInteraction studiesTailAdhesionInteractionRegulationDynamic interactionMembrane
2004
Competition for Talin Results in Trans-dominant Inhibition of Integrin Activation*
Calderwood DA, Tai V, Di Paolo G, De Camilli P, Ginsberg MH. Competition for Talin Results in Trans-dominant Inhibition of Integrin Activation*. Journal Of Biological Chemistry 2004, 279: 28889-28895. PMID: 15143061, DOI: 10.1074/jbc.m402161200.Peer-Reviewed Original ResearchConceptsTrans-dominant inhibitionIntegrin activationFragment of talinCytoskeletal protein talinIntegrin adhesion receptorsMulticellular animalsProtein talinExtracellular ligandsCellular processesBeta tailsTalinTransdominant inhibitionAdhesion receptorsDifferent integrinsOverexpression of integrinsIntegrinsActivationClot retractionInhibitionReceptorsTailAdhesionSpeciesProteinOverexpressionIntegrin activation
Calderwood DA. Integrin activation. Journal Of Cell Science 2004, 117: 657-666. PMID: 14754902, DOI: 10.1242/jcs.01014.Peer-Reviewed Original ResearchConceptsIntegrin cytoplasmic domainExtracellular matrixCell-ECM adhesionIntegrin extracellular domainCytoskeletal protein talinTransmembrane adhesion receptorsAbility of cellsMulticellular organismsProtein talinExtracellular ligandsCytoplasmic domainIntegrin activationIntracellular signalsExtracellular domainAdhesion receptorsIntegrin familyConformational changesIntegrin receptorsIntracellular stepsReversible mechanismRecent studiesAdhesionTalinReceptorsOrganisms