2019
Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior
Blanco-Centurion C, Luo S, Spergel DJ, Vidal-Ortiz A, Oprisan S, Van den Pol AN, Liu M, Shiromani PJ. Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior. Journal Of Neuroscience 2019, 39: 4986-4998. PMID: 31036764, PMCID: PMC6670248, DOI: 10.1523/jneurosci.0305-19.2019.Peer-Reviewed Original ResearchConceptsMelanin-concentrating hormoneRapid eye movement (REM) sleepMCH neuronsEye movement sleepREM sleepHypothalamic neuronsMovement sleepHypothalamic melanin-concentrating hormone (MCH) neuronsMelanin-concentrating hormone (MCH) neuronsNeuropeptide melanin-concentrating hormoneHypothalamic MCH neuronsIndividual hypothalamic neuronsMost brain neuronsFunctional activity mapsExploratory behaviorNon-REM sleepCalcium indicator GCaMP6Single neuron activityHormone neuronsNetwork activationBrain neuronsElectrophysiology studySame neuronsQuiet wakingIndicator GCaMP6s
2012
Kisspeptin Inhibits High-Voltage Activated Ca2+ Channels in GnRH Neurons via Multiple Ca2+ Influx and Release Pathways
Zhang XB, Spergel DJ. Kisspeptin Inhibits High-Voltage Activated Ca2+ Channels in GnRH Neurons via Multiple Ca2+ Influx and Release Pathways. Neuroendocrinology 2012, 96: 68-80. PMID: 22343183, DOI: 10.1159/000335985.Peer-Reviewed Original ResearchConceptsGnRH neuronsGnRH-green fluorescent protein transgenic miceG protein-coupled receptor 54Phospholipase C inhibitor edelfosineWhole-cell patch-clamp recordingsGonadotropin-releasing hormone (GnRH) secretionCanonical transient receptor potential (TRPC) channelsTransient receptor potential channelsRyR agonist caffeinePatch-clamp recordingsChelation of intracellularVGCC activityReceptor 54Kisspeptin-10Hormone secretionPeptide 234KP-10Antagonist caffeineBrain slicesSubsequent fertilityTransgenic miceTRPC channelsNeuronsDependent inhibitionCytoplasmic free
2009
Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse
Ward DR, Dear FM, Ward IA, Anderson SI, Spergel DJ, Smith PA, Ebling FJ. Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse. PLOS ONE 2009, 4: e5322. PMID: 19390688, PMCID: PMC2669176, DOI: 10.1371/journal.pone.0005322.Peer-Reviewed Original ResearchConceptsGnRH neuronsVasoactive intestinal peptidePeptidergic neuronsGonadotropin-Releasing Hormone NeuronsGnRH secretory systemVIP-IR fibersGonadotropin-releasing hormoneHypothalamic peptide systemsHormone neuronsBasal forebrainIntestinal peptideHypothalamic neuropeptidesPeptidergic fibersReproductive axisTransgenic miceModulatory inputsNeuronsCircadian timing informationPeptidergic signalsMetabolic statusMiceCircadian informationInnervationSignificant proportionPeptide system
2007
Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty
Spergel DJ. Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty. Endocrinology 2007, 148: 2383-2390. PMID: 17289846, PMCID: PMC3315592, DOI: 10.1210/en.2006-1693.Peer-Reviewed Original ResearchConceptsAdult GnRH neuronsSK channel expressionGnRH neuronsGnRH secretionSK currentPubertal increaseGnRH-green fluorescent protein transgenic miceChannel expressionSmall-conductance calcium-activated potassium channelsGonadotropin-releasing hormone (GnRH) neuronsSK channel blocker apaminCalcium-activated potassium channelsCytoplasmic freeChannel blocker apaminExcitatory synaptic inputsWhole-cell techniqueSK channel subtypesHormone neuronsBlocker apaminKisspeptin stimulationChannel blockersSK channelsBrain slicesChannel subtypesSynaptic inputs
2005
Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons.
Shimshek DR, Bus T, Grinevich V, Single FN, Mack V, Sprengel R, Spergel DJ, Seeburg PH. Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons. Endocrinology 2005, 20: 219-31. PMID: 16099814, DOI: 10.1210/me.2005-0262.Peer-Reviewed Original ResearchConceptsAMPA receptorsNMDA receptorsGnRH secretionMale miceN-methyl-D-aspartate receptorsLimbic system neuronsSerum testosterone levelsIsoxazolepropionic acid (AMPA) receptorsIonotropic glutamate receptorsNormal litter sizeMale sexual behaviorGnRH neuronsSeptal neuronsSystem neuronsOvarian morphologyGlutamate receptorsSeptal areaTestosterone levelsControl littermatesMale littermatesPup retrievalMajor subtypesTestis weightMaternal aggressionAcid receptors
1999
GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice
Spergel D, Krüth U, Hanley D, Sprengel R, Seeburg P. GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice. Journal Of Neuroscience 1999, 19: 2037-2050. PMID: 10066257, PMCID: PMC6782541, DOI: 10.1523/jneurosci.19-06-02037.1999.Peer-Reviewed Original ResearchConceptsGnRH neuronsPreoptic areaNucleated patchesWhole-cell recordingsApplication of GABANMDA receptor channelsGanglion terminaleHormone neuronsOrganum vasculosumSeptal nucleusDiagonal bandLamina terminalisNeuronal somataAxon terminalsOlfactory bulbMedian eminenceTransgenic miceFiring patternsNeuronsNeuronal dendritesReceptor channelsGABAGreen fluorescent proteinL-glutamateAMPA
1993
Optical Measurements of Micro-Vascular Oxygen Pressure and Intracellular pH in the Cat Carotid Body: Testing Hypotheses of Oxygen Chemoreception
Rumsey WL, Iturriaga R, Spergel D, Lahiri S, Wilson DF. Optical Measurements of Micro-Vascular Oxygen Pressure and Intracellular pH in the Cat Carotid Body: Testing Hypotheses of Oxygen Chemoreception. Advances In Experimental Medicine And Biology 1993, 337: 197-203. PMID: 8109402, DOI: 10.1007/978-1-4615-2966-8_28.Chapters
1992
Intracellular pH and oxygen chemoreception in the cat carotid body in vitro
Iturriaga R, Rumsey WL, Lahiri S, Spergel D, Wilson DF. Intracellular pH and oxygen chemoreception in the cat carotid body in vitro. Journal Of Applied Physiology 1992, 72: 2259-2266. PMID: 1629081, DOI: 10.1152/jappl.1992.72.6.2259.Peer-Reviewed Original ResearchOptical Measurements of Oxygen and Electrical Measurements of Oxygen Chemoreception in the Cat Carotid Body
Rumsey W, Lahiri S, Iturriaga R, Mokashi A, Spergel D, Wilson D. Optical Measurements of Oxygen and Electrical Measurements of Oxygen Chemoreception in the Cat Carotid Body. Advances In Experimental Medicine And Biology 1992, 317: 387-395. PMID: 1288149, DOI: 10.1007/978-1-4615-3428-0_43.Chapters
1991
In vitro perfused-superfused cat carotid body for physiological and pharmacological studies
Iturriaga R, Rumsey WL, Mokashi A, Spergel D, Wilson DF, Lahiri S. In vitro perfused-superfused cat carotid body for physiological and pharmacological studies. Journal Of Applied Physiology 1991, 70: 1393-1400. PMID: 1903380, DOI: 10.1152/jappl.1991.70.3.1393.Peer-Reviewed Original ResearchConceptsChemosensory dischargeCarotid bodyPentobarbital sodium-anesthetized catsWhole carotid sinus nerveCarotid sinus nerveInjections of nicotineCarotid body preparationCat carotid bodyRestoration of flowDose-dependent mannerSinus nerveNormoxic perfusionPerfusate PO2Pharmacological stimuliPerfusate flowInitial responsivenessTyrode's solutionPharmacological studiesCarotid bifurcationPerfusionCO2 stimulusBasal activityChemosensory responsesNicotineBody preparation