1996
Immortalized GnRH Neurons Express Large-Conductance Calcium-Activated Potassium Channels
Spergel D, Catt K, Rojas E. Immortalized GnRH Neurons Express Large-Conductance Calcium-Activated Potassium Channels. Neuroendocrinology 1996, 63: 101-111. PMID: 9053774, DOI: 10.1159/000126946.Peer-Reviewed Original ResearchConceptsGT1-7 cellsGnRH secretory responseGnRH secretionChannel blockersBK channelsSecretory responseGonadotropin-releasing hormone (GnRH) neuronsEffects of charybdotoxinImmortalized GnRH neuronsPerforated-patch recordingsSmall conductance Ca2Patch-clamp recordingsBK channel blockersGamma-aminobutyric acidPatch-clamp techniqueLarge-conductance Ca2Cytoplasmic free Ca2Endoplasmic reticulum Ca2Hormone neuronsGnRH neuronsExcitatory neurotransmitterVoltage-dependent mannerSustained componentFura-2Whole-cell conductance
1995
L-Type Ca2+ Channels Mediate Joint Modulation by Gamma-Amino-butyric Acid and Glutamate of [Ca2+]i and Neuropeptide Secretion in Immortalized Gonadodropin-Releasing Hormone Neurons
Spergel D, Krsmanovic L, Stojilkovic S, Catt K. L-Type Ca2+ Channels Mediate Joint Modulation by Gamma-Amino-butyric Acid and Glutamate of [Ca2+]i and Neuropeptide Secretion in Immortalized Gonadodropin-Releasing Hormone Neurons. Neuroendocrinology 1995, 61: 499-508. PMID: 7617127, DOI: 10.1159/000126873.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidReceptor antagonistReceptor agonistSecretory responseGonadotropin-releasing hormone (GnRH) secretionGlutamate-induced Ca2GnRH secretory responseImmortalized GnRH neuronsGABAB receptor antagonistGABAA receptor antagonistGABAB receptor agonistGABAA receptor agonistL-type Ca2GT1-7 neuronsHormone neuronsGnRH neuronsRole of calciumHormone secretionGamma-AminoNeuropeptide secretionAgonist concentrationsCytoplasmic calciumNeuronsFree mediumGlutamate