A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression
Bergwitz C, Wendlandt T, Pötter E, Glomb I, Gras K, von zur Mühlen A, Brabant G. A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression. Histochemistry And Cell Biology 2000, 113: 145-150. PMID: 10766267, DOI: 10.1007/s004180050017.Peer-Reviewed Original ResearchConceptsTetracycline-regulated gene expressionGene expressionTetracycline-regulated gene expression systemStable transfectionChondrogenic differentiationGene expression systemGene of interestBasis of phenotypeTetracycline transactivatorGenetic manipulationHygromycin selectionExpression systemCartilage-specific proteoglycansNeomycin selectionFlexible genetic manipulationLuciferase activityType II expressionCell linesAlcian blue stainingDifferentiationCollagen type II expressionTransfectionExpressionBlue stainingGenes