2001
Wnts differentially regulate colony growth and differentiation of chondrogenic rat calvaria cells
Bergwitz C, Wendlandt T, Kispert A, Brabant G. Wnts differentially regulate colony growth and differentiation of chondrogenic rat calvaria cells. Biochimica Et Biophysica Acta 2001, 1538: 129-140. PMID: 11336784, DOI: 10.1016/s0167-4889(00)00123-3.Peer-Reviewed Original ResearchConceptsChondrogenic cell lineReporter gene expressionWnt effectsGene expressionCell linesWnt-7aChondrogenic differentiationWnt-5aGreen fluorescence proteinProtein kinase C.Protein kinase CFrizzled-related proteinsCollagen type II promoterLimb patterningSignal transductionEmbryonic developmentRat calvaria cellsWnt familyType II promoterNIH3T3 cellsReporter systemCartilage-specific proteoglycansFluorescence proteinCartilage differentiationKinase C.
2000
A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression
Bergwitz C, Wendlandt T, Pötter E, Glomb I, Gras K, von zur Mühlen A, Brabant G. A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression. Histochemistry And Cell Biology 2000, 113: 145-150. PMID: 10766267, DOI: 10.1007/s004180050017.Peer-Reviewed Original ResearchConceptsTetracycline-regulated gene expressionGene expressionTetracycline-regulated gene expression systemStable transfectionChondrogenic differentiationGene expression systemGene of interestBasis of phenotypeTetracycline transactivatorGenetic manipulationHygromycin selectionExpression systemCartilage-specific proteoglycansNeomycin selectionFlexible genetic manipulationLuciferase activityType II expressionCell linesAlcian blue stainingDifferentiationCollagen type II expressionTransfectionExpressionBlue stainingGenes