Featured Publications
ADAMTS-7 forms a positive feedback loop with TNF-α in the pathogenesis of osteoarthritis
Lai Y, Bai X, Zhao Y, Tian Q, Liu B, Lin E, Chen Y, Lee B, Appleton C, Beier F, Yu X, Liu C. ADAMTS-7 forms a positive feedback loop with TNF-α in the pathogenesis of osteoarthritis. Annals Of The Rheumatic Diseases 2013, 73: 1575. PMID: 23928557, PMCID: PMC4418017, DOI: 10.1136/annrheumdis-2013-203561.Peer-Reviewed Original ResearchConceptsADAMTS-7ADAMTS-7 expressionRat OA modelPathogenesis of osteoarthritisNF-κB signalingOA modelOA progressionCartilage degradationOA-like phenotypeTumor necrosis factorProgression of osteoarthritisJoint degenerative diseaseDownstream NF-κB signalingPotential molecular targetsPositive feedback loopShort-limbed dwarfismDisease progressionOA developmentNecrosis factorSafranin O stainingYoung miceUpregulated TNFReporter gene assayOsteoarthritisTransgenic mice
2017
Progranulin derivative Atsttrin protects against early osteoarthritis in mouse and rat models
Wei J, Fu W, Ding Y, Hettinghouse A, Lendhey M, Schwarzkopf R, Kennedy O, Liu C. Progranulin derivative Atsttrin protects against early osteoarthritis in mouse and rat models. Arthritis Research & Therapy 2017, 19: 280. PMID: 29258611, PMCID: PMC5735869, DOI: 10.1186/s13075-017-1485-8.Peer-Reviewed Original ResearchConceptsTherapeutic effectOA modelPain-related markersAnti-catabolic effectsRole of progranulinDegenerative joint diseaseMurine OA modelMultiple murine modelsNew therapeutic alternativesTumor necrosis factor receptorIntra-articular deliveryNecrosis factor receptorInflammatory arthritisPGRN deficiencyOA phenotypeSafranin O stainingTherapeutic alternativeRat modelJoint diseaseOsteoarthritis progressionPreventative effectMurine modelCartilage degradationDegenerative factorsEarly osteoarthritis
2011
Cartilage Oligomeric Matrix Protein Inhibits Vascular Smooth Muscle Calcification by Interacting With Bone Morphogenetic Protein-2
Du Y, Wang Y, Wang L, Liu B, Tian Q, Liu C, Zhang T, Xu Q, Zhu Y, Ake O, Qi Y, Tang C, Kong W, Wang X. Cartilage Oligomeric Matrix Protein Inhibits Vascular Smooth Muscle Calcification by Interacting With Bone Morphogenetic Protein-2. Circulation Research 2011, 108: 917-928. PMID: 21350216, DOI: 10.1161/circresaha.110.234328.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsCartilage oligomeric matrix proteinVascular calcificationOligomeric matrix proteinVSMC calcificationVascular smooth muscle calcificationMarker expressionHigh-phosphate dietSmooth muscle cellsCOMP deficiencyMuscle calcificationCardiovascular morbidityOsteogenic marker expressionAbdominal aortaVessel calcificationHigh inorganic phosphateEffects of COMPPeriadventitial applicationMatrix proteinsMuscle cellsCalcificationBMP-2Receptor bindingProtein levelsOsteogenic signaling
2009
Cartilage Oligomeric Matrix Protein Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells by Interacting With &agr;7&bgr;1 Integrin
Wang L, Zheng J, Du Y, Huang Y, Li J, Liu B, Liu C, Zhu Y, Gao Y, Xu Q, Kong W, Wang X. Cartilage Oligomeric Matrix Protein Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells by Interacting With &agr;7&bgr;1 Integrin. Circulation Research 2009, 106: 514-525. PMID: 20019333, DOI: 10.1161/circresaha.109.202762.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsAntigens, CDAorta, ThoracicBecaplerminCarotid Artery InjuriesCatheterizationCell DifferentiationCells, CulturedExtracellular MatrixExtracellular Matrix ProteinsGene Knockdown TechniquesGenetic VectorsGlycoproteinsIntegrin alpha ChainsIntegrinsMaleMatrilin ProteinsMuscle ContractionMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MusclePhenotypePlatelet-Derived Growth FactorProtein Interaction MappingProto-Oncogene Proteins c-sisRatsRats, Sprague-DawleyRecombinant Fusion ProteinsRNA, Small InterferingConceptsVascular smooth muscle cellsCartilage oligomeric matrix proteinEffects of COMPContractile phenotypeFocal adhesion assemblyVSMC differentiation marker genesAdenoviral overexpressionSmooth muscle cellsNormal vascular smooth muscle cellsVSMC phenotype switchingDifferentiation marker genesActin fiber organizationMuscle cellsAdhesion assemblyVascular extracellular matrixVSMC contractile phenotypeMarker genesDifferentiated stateRat vascular smooth muscle cellsPhenotype switchingPlatelet-derived growthVSMC adhesionMatrix proteinsVSMC dedifferentiationSmall interferingADAMTS-7 Mediates Vascular Smooth Muscle Cell Migration and Neointima Formation in Balloon-Injured Rat Arteries
Wang L, Zheng J, Bai X, Liu B, Liu C, Xu Q, Zhu Y, Wang N, Kong W, Wang X. ADAMTS-7 Mediates Vascular Smooth Muscle Cell Migration and Neointima Formation in Balloon-Injured Rat Arteries. Circulation Research 2009, 104: 688-698. PMID: 19168437, DOI: 10.1161/circresaha.108.188425.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAMTS7 ProteinAnimalsBecaplerminCarotid ArteriesCarotid Artery InjuriesCatheterizationCell MovementCells, CulturedDisease Models, AnimalExtracellular Matrix ProteinsGlycoproteinsHyperplasiaMaleMatrilin ProteinsMuscle, Smooth, VascularMyocytes, Smooth MuscleNF-kappa BPlatelet-Derived Growth FactorProto-Oncogene Proteins c-sisRatsRats, Sprague-DawleyRNA InterferenceRNA, Small InterferingTime FactorsTranscription Factor AP-1Transduction, GeneticTumor Necrosis Factor-alphaTunica IntimaConceptsVascular smooth muscle cellsCartilage oligomeric matrix proteinADAMTS-7VSMC migrationIntimal thickeningNeointima formationProinflammatory cytokine tumor necrosis factor alphaCytokine tumor necrosis factor alphaTumor necrosis factor alphaADAMTS-7 levelsVascular smooth muscle cell migrationDevelopment of atherosclerosisPrimary vascular smooth muscle cellsSmooth muscle cell migrationNecrosis factor alphaNovel therapeutic targetSmooth muscle cellsMuscle cell migrationPlatelet-derived growthFamily of proteinasesMigration/invasionOligomeric matrix proteinPerivascular administrationBalloon injuryVascular injury
2008
Regulation of chondrocyte differentiation by ADAMTS-12 metalloproteinase depends on its enzymatic activity
Bai X, Wang D, Luan Y, Yu X, Liu C. Regulation of chondrocyte differentiation by ADAMTS-12 metalloproteinase depends on its enzymatic activity. Cellular And Molecular Life Sciences 2008, 66: 667. PMID: 19151918, PMCID: PMC11131527, DOI: 10.1007/s00018-008-8633-x.Peer-Reviewed Original ResearchConceptsADAMTS-12Chondrocyte differentiationGrowth plate chondrocytesType X collagen expressionThrombospondin motifsPTHrPCollagen expressionAltered expressionMRNA levelsProminent expressionDownstream moleculesADAMTS familyMetalloproteinaseInhibitionEnzymatic activityNovel regulatorProteolytic activityChondrocytesExpressionChondrogenesisDifferentiationActivity
2007
Cartilage Oligomeric Matrix Protein Associates with Granulin-Epithelin Precursor (GEP) and Potentiates GEP-stimulated Chondrocyte Proliferation*
Xu K, Zhang Y, Ilalov K, Carlson C, Feng J, Di Cesare P, Liu C. Cartilage Oligomeric Matrix Protein Associates with Granulin-Epithelin Precursor (GEP) and Potentiates GEP-stimulated Chondrocyte Proliferation*. Journal Of Biological Chemistry 2007, 282: 11347-11355. PMID: 17307734, DOI: 10.1074/jbc.m608744200.Peer-Reviewed Original ResearchConceptsGranulin-epithelin precursorCartilage oligomeric matrix proteinMutant cartilage oligomeric matrix proteinTwo-hybrid systemCo-immunoprecipitation assaysFunctional genetic screensChondrocyte proliferationHuman cartilage oligomeric matrix proteinGenetic screenRat chondrosarcoma cellsProtein associatesGrowth factorRepeat domainBiological functionsFunctional domainsMouse embryosMatrix proteinsAnti-COMP antibodiesChondrosarcoma cellsOligomeric matrix proteinAutocrine growth factorMultiple epiphyseal dysplasiaPericellular matrixPrimary human chondrocytesRepeat units
2006
ADAMTS‐7: a metalloproteinase that directly binds to and degrades cartilage oligomeric matrix protein
Liu C, Kong W, Ilalov K, Yu S, Xu K, Prazak L, Fajardo M, Sehgal B, Di Cesare P, Liu C, Kong W, Ilalov K, Yu S, Xu K, Prazak L, Fajardo M, Sehgal B, Di Cesare P. ADAMTS‐7: a metalloproteinase that directly binds to and degrades cartilage oligomeric matrix protein. The FASEB Journal 2006, 20: 988-990. PMID: 16585064, PMCID: PMC1483927, DOI: 10.1096/fj.05-3877fje.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAMTS7 ProteinAdultAgedAnimalsArthritis, RheumatoidBinding SitesCartilageCartilage Oligomeric Matrix ProteinExtracellular Matrix ProteinsGene Expression Regulation, EnzymologicGlycoproteinsHumansHydrogen-Ion ConcentrationMatrilin ProteinsMiceMiddle AgedProtein BindingRatsRNA, MessengerSynovial MembraneTwo-Hybrid System TechniquesUp-RegulationZincConceptsCartilage oligomeric matrix proteinMatrix proteinsEpidermal growth factor domainTwo-hybrid screenOligomeric matrix proteinGrowth factor domainRecombinant catalytic domainCatalytic domainRepeat domainFunctional domainsInteraction partnersADAMTS-7Factor domainAmino acidsEnzymatic activityProteinHigh expression