2023
Streptococcus pyogenes Colonization in Children Aged 24–59 Months in the Gambia: Impact of Live Attenuated Influenza Vaccine and Associated Serological Responses
Keeley A, Groves D, Armitage E, Senghore E, Jagne Y, Sallah H, Drammeh S, Angyal A, Hornsby H, de Crombrugghe G, Smeesters P, Rossi O, Carducci M, Peno C, Bogaert D, Kampmann B, Marks M, Shaw H, Turner C, de Silva T. Streptococcus pyogenes Colonization in Children Aged 24–59 Months in the Gambia: Impact of Live Attenuated Influenza Vaccine and Associated Serological Responses. The Journal Of Infectious Diseases 2023, 228: 957-965. PMID: 37246259, PMCID: PMC10547459, DOI: 10.1093/infdis/jiad153.Peer-Reviewed Original ResearchConceptsQuantitative polymerase chain reactionLAIV groupInfluenza vaccineSerological responseOdds ratioDay 7Live attenuated influenza vaccineChildren Aged 24High-burden settingsAttenuated influenza vaccineControl group participantsBurden settingsGambian childrenNasopharyngeal swabsPoint prevalenceAged 24Polymerase chain reactionAsymptomatic colonizationNasopharyngeal colonizationIgG increaseControl groupDay 21Day 0LAIVS. pyogenesRoutine saliva testing for SARS-CoV-2 in children: Methods for partnering with community childcare centers
Rayack E, Askari H, Zirinsky E, Lapidus S, Sheikha H, Peno C, Kazemi Y, Yolda-Carr D, Liu C, Grubaugh N, Ko A, Wyllie A, Spatz E, Oliveira C, Bei A. Routine saliva testing for SARS-CoV-2 in children: Methods for partnering with community childcare centers. Frontiers In Public Health 2023, 11: 1003158. PMID: 36817891, PMCID: PMC9936085, DOI: 10.3389/fpubh.2023.1003158.Peer-Reviewed Original ResearchConceptsParents/guardiansOnline patient portalYounger age groupsSARS-CoV-2Age groupsSurveillance programSaliva collectionSARS-CoV-2 testingSARS-CoV-2 screeningWeekly saliva samplesRT-PCR testingChildcare centre staffCritical age groupRoutine surveillance toolRoutine testing programsChildcare centersCOVID-19 transmissionAsymptomatic screeningSaliva collection methodNasal swabsPatient portalsSymptomatic testingPublic health dataSaliva samplesChildcare facilities
2022
Effect of intra-partum azithromycin on the development of the infant nasopharyngeal microbiota: A post hoc analysis of a double-blind randomized trial
Sanyang B, de Silva T, Kanteh A, Bojang A, Manneh J, de Steenhuijsen Piters W, Peno C, Bogaert D, Sesay A, Roca A. Effect of intra-partum azithromycin on the development of the infant nasopharyngeal microbiota: A post hoc analysis of a double-blind randomized trial. EBioMedicine 2022, 83: 104227. PMID: 35988464, PMCID: PMC9420482, DOI: 10.1016/j.ebiom.2022.104227.Peer-Reviewed Original ResearchConceptsNasopharyngeal microbiotaDay 6Neonatal periodDouble-blind randomized placebo-controlled clinical trialRandomized placebo-controlled clinical trialPlacebo-controlled clinical trialEarly antibiotic exposureMonths of ageMedical Research CouncilOverall microbiota compositionOral azithromycinPlacebo armNasopharyngeal carriageHealthy infantsModest overall effectNeonatal deathNasopharyngeal samplesMicrobiota perturbationsClinical trialsLeading causeAntibiotic exposureBacterial carriageShort-term alterationsMicrobiota compositionAzithromycin
2021
The effect of live attenuated influenza vaccine on pneumococcal colonisation densities among children aged 24–59 months in The Gambia: a phase 4, open label, randomised, controlled trial
Peno C, Armitage E, Clerc M, Balcazar Lopez C, Jagne Y, Drammeh S, Jarju S, Sallah H, Senghore E, Lindsey B, Camara J, Bah S, Mohammed N, Dockrell D, Kampmann B, Clarke E, Bogaert D, de Silva T. The effect of live attenuated influenza vaccine on pneumococcal colonisation densities among children aged 24–59 months in The Gambia: a phase 4, open label, randomised, controlled trial. The Lancet Microbe 2021, 2: e656-e665. PMID: 34881370, PMCID: PMC8632704, DOI: 10.1016/s2666-5247(21)00179-8.Peer-Reviewed Original ResearchConceptsPneumococcal densityRespiratory viral infectionsLAIV groupMiddle-income country populationsPneumococcal carriageRespiratory virusesControl groupDay 7Influenza vaccineViral infectionDay 21Asymptomatic respiratory viral infectionsHigher pneumococcal carriage ratesHigh pneumococcal carriageInfluenza vaccine programsNasopharyngeal pneumococcal carriagePneumococcal carriage rateUse of LAIVAttenuated influenza vaccineUpper respiratory tractInvestigator teamOpen labelRandomisation sequencePrimary outcomeCarriage prevalenceGenomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa
Cornick J, Musicha P, Peno C, Seager E, Tam P, Bilima S, Bennett A, Kennedy N, Feasey N, Heinz E, Cain A. Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa. Microbial Genomics 2021, 7: 000703. PMID: 34793293, PMCID: PMC8743538, DOI: 10.1099/mgen.0.000703.Peer-Reviewed Original ResearchConceptsStrain sequence typesBloodstream infectionsSpecial Care Neonatal UnitNeonatal care unitImportant hospital pathogensKlebsiella pneumoniae outbreakLarge public hospitalCare unitNeonatal unitPediatric wardNeonatal wardHospital outbreaksTreat infectionsHospital pathogensPublic hospitalsPlasmid typesHospitalWhole-genome sequencingInfectionSequence typesWardsYear periodSaharan AfricaOutbreakLikely cause
2020
Bacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism
Chaguza C, Yang M, Cornick JE, du Plessis M, Gladstone RA, Kwambana-Adams BA, Lo SW, Ebruke C, Tonkin-Hill G, Peno C, Senghore M, Obaro SK, Ousmane S, Pluschke G, Collard JM, Sigaùque B, French N, Klugman KP, Heyderman RS, McGee L, Antonio M, Breiman RF, von Gottberg A, Everett DB, Kadioglu A, Bentley SD. Bacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism. Communications Biology 2020, 3: 559. PMID: 33033372, PMCID: PMC7545184, DOI: 10.1038/s42003-020-01290-9.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesPneumococcal serotype 1Genetic variationBacterial genome-wide association studiesAssociation studiesSerotype 1Central nervous system infectionSignificant genotype-phenotype associationsNervous system infectionGenotype-phenotype associationsHelicase proteinPopulation structureCNS infectionsSystem infectionNegligible heritabilityMeningitis outbreakCNS tissueCerebrospinal fluidImmune evasionSerotype 1 strainPneumococcal survivalPolygenic etiologySmall effect sizesMeningitisTropismNiche- and Gender-Dependent Immune Reactions in Relation to the Microbiota Profile in Pediatric Patients with Otitis Media with Effusion
Enoksson F, Rodriguez A, Peno C, Lopez C, Tjernström F, Bogaert D, Hakansson A, Bergenfelz C. Niche- and Gender-Dependent Immune Reactions in Relation to the Microbiota Profile in Pediatric Patients with Otitis Media with Effusion. Infection And Immunity 2020, 88: e00147-20. PMID: 32661126, PMCID: PMC7504947, DOI: 10.1128/iai.00147-20.Peer-Reviewed Original ResearchConceptsMiddle earInflammatory mediatorsOtitis mediaImmune responseChronic low-grade inflammationMiddle ear effusion samplesLow-grade inflammationSpecific inflammatory mediatorsAnti-inflammatory mediatorsAnti-inflammatory profileCommon inflammatory diseaseSigns of infectionHost immune responsePotential pathogenic mechanismsInflammation patternsMale patientsPediatric patientsProinflammatory mediatorsFemale patientsPrecise pathogenesisProinflammatory profileInflammatory diseasesT lymphocytesImmune reactionsPathogenic mechanisms
2019
Early Signals of Vaccine-driven Perturbation Seen in Pneumococcal Carriage Population Genomic Data
Chaguza C, Heinsbroek E, Gladstone RA, Tafatatha T, Alaerts M, Peno C, Cornick JE, Musicha P, Bar-Zeev N, Kamng’ona A, Kadioglu A, McGee L, Hanage WP, Breiman RF, Heyderman RS, French N, Everett DB, Bentley SD. Early Signals of Vaccine-driven Perturbation Seen in Pneumococcal Carriage Population Genomic Data. Clinical Infectious Diseases 2019, 70: 1294-1303. PMID: 31094423, PMCID: PMC7768739, DOI: 10.1093/cid/ciz404.Peer-Reviewed Original ResearchConceptsYears of ageVaccine serotypesWhole-genome sequencingHigh disease burdenAntibiotic resistanceLow-income settingsPCV introductionReplacement serotypesVT serotypesPneumococcal diseaseNonvaccine serotypesCarriage rateDisease burdenPneumococcal isolatesSerotype dynamicsContinued surveillancePCV effectsAge groupsSerotypesSerotype diversityAgeVaccineResistant lineagesPCVChildren
2017
Population genetic structure, antibiotic resistance, capsule switching and evolution of invasive pneumococci before conjugate vaccination in Malawi
Chaguza C, Cornick JE, Andam CP, Gladstone RA, Alaerts M, Musicha P, Peno C, Bar-Zeev N, Kamng'ona AW, Kiran AM, Msefula CL, McGee L, Breiman RF, Kadioglu A, French N, Heyderman RS, Hanage WP, Bentley SD, Everett DB. Population genetic structure, antibiotic resistance, capsule switching and evolution of invasive pneumococci before conjugate vaccination in Malawi. Vaccine 2017, 35: 4594-4602. PMID: 28711389, PMCID: PMC5571440, DOI: 10.1016/j.vaccine.2017.07.009.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultBacterial CapsulesChildChild, PreschoolDrug Resistance, Multiple, BacterialEvolution, MolecularFemaleGenome, BacterialHigh-Throughput Nucleotide SequencingHumansInfantInfant, NewbornMalawiMalePneumococcal InfectionsPneumococcal VaccinesPrevalenceRetrospective StudiesSerogroupSerotypingStreptococcus pneumoniaeVaccinationVaccines, ConjugateYoung AdultConceptsPneumococcal conjugate vaccineMDR ratePneumococcal isolatesHigher MDR rateImplementation of PCV13Prevalence of serotypesNon-vaccine serotypesInvasive pneumococcal isolatesGood infection preventionAntibiotic resistance ratesAntibiotic resistanceAbsence of vaccinesReplacement serotypesSerotype replacementInvasive pneumococciPneumococcal infectionConjugate vaccineSerotype 12FDisease burdenInfection preventionSerotype prevalenceHigh prevalenceInvasive isolatesContinued surveillanceResistance ratesGenomic landscape of extended-spectrum β-lactamase resistance in Escherichia coli from an urban African setting
Musicha P, Feasey NA, Cain AK, Kallonen T, Chaguza C, Peno C, Khonga M, Thompson S, Gray KJ, Mather AE, Heyderman RS, Everett DB, Thomson NR, Msefula CL. Genomic landscape of extended-spectrum β-lactamase resistance in Escherichia coli from an urban African setting. Journal Of Antimicrobial Chemotherapy 2017, 72: 1602-1609. PMID: 28333330, PMCID: PMC5437524, DOI: 10.1093/jac/dkx058.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultBeta-Lactam ResistanceBeta-LactamasesChildChild, PreschoolChloramphenicolDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli InfectionsFemaleGenes, BacterialGenetic VariationGenomicsHumansMalawiMaleMicrobial Sensitivity TestsMultilocus Sequence TypingPhylogenyUrban PopulationYoung AdultConceptsAntimicrobial resistanceExtended spectrum β-lactamase resistanceEscherichia coli infectionResource-rich settingsUrban African settingCommon ESBL geneLow-income settingsPhenotypic resistance profilesΒ-lactamase resistanceColi infectionESBL resistanceGlobal epidemiologyFluoroquinolone resistanceQueen's HospitalBla CTXESBL genesMolecular epidemiologyResistance profilesMolecular patternsAfrican settingClinical sourcesHospitalE. coliEpidemiologyST131