2024
SINE RNA of the imprinted miRNA clusters mediates constitutive type III interferon expression and antiviral protection in hemochorial placentas
Wickramage I, VanWye J, Max K, Lockhart J, Hortu I, Mong E, Canfield J, Lamabadu Warnakulasuriya Patabendige H, Guzeloglu-Kayisli O, Inoue K, Ogura A, Lockwood C, Akat K, Tuschl T, Kayisli U, Totary-Jain H. SINE RNA of the imprinted miRNA clusters mediates constitutive type III interferon expression and antiviral protection in hemochorial placentas. Cell Host & Microbe 2024 PMID: 39396520, DOI: 10.1016/j.chom.2024.10.003.Peer-Reviewed Original ResearchENHANCED FKBP51-GLUCOCORTICOID SIGNALING INDUCES LEIOMYOMA CELLS TOWARD A MYOFIBROBLAST PHENOTYPE
Malcom C, Guzeloglu-Kayisli O, Un B, New E, Guo X, Imudia A, Lockwood C, Kayisli U. ENHANCED FKBP51-GLUCOCORTICOID SIGNALING INDUCES LEIOMYOMA CELLS TOWARD A MYOFIBROBLAST PHENOTYPE. Fertility And Sterility 2024, 122: e106. DOI: 10.1016/j.fertnstert.2024.07.354.Peer-Reviewed Original ResearchDIMINISHED FOLLICULAR FLUID SOLUBLE ENDOGLIN LEVEL IN WOMEN WITH LOW OOCYTE YIELD
Sarkar P, Moore M, Larsen K, Semerci N, Gokturk A, Alhasan H, Imudia A, Kayisli U, Lockwood C, Guzeloglu-Kayisli O. DIMINISHED FOLLICULAR FLUID SOLUBLE ENDOGLIN LEVEL IN WOMEN WITH LOW OOCYTE YIELD. Fertility And Sterility 2024, 122: e63-e64. DOI: 10.1016/j.fertnstert.2024.05.113.Peer-Reviewed Original ResearchDecidual Cells Block Inflammation-Mediated Inhibition of 15-Hydroxyprostaglandin Dehydrogenase in Trophoblasts
de Assis V, Kayisli U, Ozmen A, Semerci N, Totary-Jain H, Pakasticali N, Halade G, Lockwood C, Guzeloglu-Kayisli O. Decidual Cells Block Inflammation-Mediated Inhibition of 15-Hydroxyprostaglandin Dehydrogenase in Trophoblasts. American Journal Of Pathology 2024, 194: 1724-1736. PMID: 38879084, DOI: 10.1016/j.ajpath.2024.05.005.Peer-Reviewed Original ResearchConditioned medium supernatantTerm decidual cellsDecidual cellsExtravillous trophoblastHPGD expressionGestational age-matched controlsHuman trophoblast stem cellsFetal membrane ruptureMaternal-fetal interfaceTrophoblast stem cellsAge-matched controlsDecidua parietalisCervical ripeningUterine quiescencePreterm birthUterine contractionsAnchoring villiPrimary trophoblastsParacrine mechanismsParacrine inhibitionPregnancy maintenanceIL-1BInactivate prostaglandinsStem cellsProstaglandin
2023
Career Reflections: Delivering Excellence by Building Great Teams
Lockwood C. Career Reflections: Delivering Excellence by Building Great Teams. Clinical Obstetrics & Gynecology 2023, 67: 321-325. PMID: 38149681, DOI: 10.1097/grf.0000000000000849.Peer-Reviewed Original ResearchLeptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis
Ozmen A, Nwabuobi C, Tang Z, Guo X, Larsen K, Guller S, Blas J, Moore M, Kayisli U, Lockwood C, Guzeloglu-Kayisli O. Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis. International Journal Of Molecular Sciences 2023, 25: 135. PMID: 38203306, PMCID: PMC10778808, DOI: 10.3390/ijms25010135.Peer-Reviewed Original ResearchConceptsIL-6 expressionHofbauer cellsP65 NF-κBIL-6NF-κBLeptin levelsPreeclampsia pathogenesisElevated serum interleukin-6 levelsSerum interleukin-6 levelsERK1/2 MAPKInterleukin-6 levelsPro-inflammatory phenotypeAnti-inflammatory phenotypeLeptin receptor expressionIL-6 productionPathogenesis of PEPlacental leptin productionPhosphorylation levelsERK1/2 MAPK inhibitorIL-6 regulationConcentration-dependent mannerPE patientsHuman pregnancySecond trimesterLeptin concentrationsPathogenesis and Prediction of Preterm Delivery
Kendle A, Buhimschi C, Lockwood C. Pathogenesis and Prediction of Preterm Delivery. 2023, 344-360. DOI: 10.1002/9781119636540.ch40.Peer-Reviewed Original ResearchPreterm birthPR expressionPlacental corticotropin-releasing hormonePrediction of PTBAdrenal (HPA) axis pathwayCorticotropin-releasing hormoneToll-like receptorsProtease-activated receptorsPreterm deliveryProgesterone receptor inhibitorsDecidual hemorrhageInflammatory cascadeProinflammatory cytokinesInflammatory chemokinesProgesterone activityInterleukin-1βSonographic markersReceptor inhibitorsCortisol releaseInflammation pathwaysMatrix metalloproteinasesAxis pathwayPremature activationBiochemical remodelingProgressive withdrawalDysregulated expression of GATA2 and GATA6 transcription factors in adenomyosis: implications for impaired endometrial receptivity
Pavlovic Z, Hsin-Yu Pai A, Hsiao T, Yen C, Alhasan H, Ozmen A, New E, Guo X, Imudia A, Guzeloglu-Kayisli O, Lockwood C, Kayisli U. Dysregulated expression of GATA2 and GATA6 transcription factors in adenomyosis: implications for impaired endometrial receptivity. F&S Science 2023, 5: 92-103. PMID: 37972693, DOI: 10.1016/j.xfss.2023.11.003.Peer-Reviewed Original ResearchHuman endometrial stromal cellsInsulin-like growth factor-binding protein-1Estrogen receptor 1Leukemia inhibitory factorEndometrial stromal cellsProgesterone receptorControl patientsEndometrial sectionsGATA2 overexpressionH-scoreMRNA levelsInhibitory factorStromal cellsGrowth factor-binding protein-1Effect of adenomyosisReproductive-age patientsImpaired endometrial receptivityPotential therapeutic targetYears of ageMessenger RNA levelsEndometrial implantationWestern blot analysisAdenomyosis patientsLIF levelsAge patients
2009
Progestin and Thrombin Regulate Tissue Factor Expression in Human Term Decidual Cells
Lockwood CJ, Murk W, Kayisli UA, Buchwalder LF, Huang S, Funai EF, Krikun G, Schatz F. Progestin and Thrombin Regulate Tissue Factor Expression in Human Term Decidual Cells. The Journal Of Clinical Endocrinology & Metabolism 2009, 94: 2164-2170. PMID: 19276228, PMCID: PMC2690421, DOI: 10.1210/jc.2009-0065.Peer-Reviewed Original ResearchConceptsMaternal-fetal interfaceTerm decidual cellsDecidual cellsTNF-alphaTissue factorPlacental sectionsTF mRNA levelsIL-1betaHuman maternal-fetal interfaceTF expressionWestern blottingTF levelsVillous mesenchymal cellsRT-PCRTerm placental sectionsMRNA levelsHigher histologic scoresTissue factor expressionIL-1beta effectReal-time RT-PCRQuantitative real-time RT-PCRDecidual sectionsHemostatic protectionPreterm deliveryQuantitative RT-PCRImpact of a comprehensive patient safety strategy on obstetric adverse events
Pettker CM, Thung SF, Norwitz ER, Buhimschi CS, Raab CA, Copel JA, Kuczynski E, Lockwood CJ, Funai EF. Impact of a comprehensive patient safety strategy on obstetric adverse events. American Journal Of Obstetrics And Gynecology 2009, 200: 492.e1-492.e8. PMID: 19249729, DOI: 10.1016/j.ajog.2009.01.022.Peer-Reviewed Original ResearchConceptsObstetric adverse eventsAdverse Outcome IndexAdverse eventsOutcome indexPatient safety strategiesPatient safety committeePatient safety interventionsNumber of deliveriesAdverse outcomesObstetric servicesTotal deliveriesNurse positionsPatient safetySafety interventionsSafety CommitteeInterventionOutcomesSignificant improvementExpert reviewSafety climateComprehensive strategyDeliverySafety strategiesProtocol standardization
2008
Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia
Guller S, Buhimschi CS, Y Y, Huang T, Yang L, Kuczynski E, Zambrano E, Lockwood CJ, Buhimschi IA. Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia. Laboratory Investigation 2008, 88: 1057-1067. PMID: 18679377, PMCID: PMC2682720, DOI: 10.1038/labinvest.2008.74.Peer-Reviewed Original ResearchConceptsPlasminogen activator inhibitor-1Severe preeclampsiaReperfusion injurySFlt-1Soluble fms-like tyrosine kinase-1Fms-like tyrosine kinase-1Ischemia/reperfusion injuryLife-threatening syndromeSubsequent reperfusion injuryTerm control groupPathophysiology of preeclampsiaTyrosine kinase-1Activator inhibitor-1Release of factorsSignificant increasePresence of mRNAUpregulation of mRNACeruloplasmin mRNAPlacental damagePlacental factorsMicroarray gene profilingEndothelium dysfunctionPE placentasQuantitative real-time PCRIntervillous spacePreeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells
Lockwood CJ, Yen CF, Basar M, Kayisli UA, Martel M, Buhimschi I, Buhimschi C, Huang SJ, Krikun G, Schatz F. Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells. American Journal Of Pathology 2008, 172: 1571-1579. PMID: 18467705, PMCID: PMC2408417, DOI: 10.2353/ajpath.2008.070629.Peer-Reviewed Original ResearchConceptsIL-6 levelsIL-6Decidual cellsGestational age-matched control placentasElevated IL-6 levelsIL-6 mRNA levelsCommon pregnancy disorderGenesis of preeclampsiaUteroplacental blood flowFetal-placental unitInterleukin-6 levelsPathogenesis of preeclampsiaPro-inflammatory cytokinesEndothelial cell dysfunctionInterleukin-6 expressionHuman decidual cellsReal-time quantitative RT-PCRTime quantitative RT-PCRQuantitative RT-PCRFetal morbidityDecidual macrophagesPreeclamptic womenSystemic inflammationPregnancy disordersControl placentasDecidual Cell‐expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia‐related Angiogenesis
Lockwood CJ, Krikun G, Caze R, Rahman M, Buchwalder LF, Schatz F. Decidual Cell‐expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia‐related Angiogenesis. Annals Of The New York Academy Of Sciences 2008, 1127: 67-72. PMID: 18443332, DOI: 10.1196/annals.1434.013.Peer-Reviewed Original ResearchConceptsDecidual cell-expressed tissue factorUteroplacental blood flowVascular endothelial growth factorTissue factorDecidual cellsBlood flowFirst trimester decidual cellsGrowth factorPlacental growth factorProtease-activated receptorsSFlt-1 expressionEndothelial growth factorPredominant cell typeMaternal thrombophiliaMaternal morbidityDecidual hemorrhageSpiral arteriesHuman pregnancySFlt-1Trophoblast invasionVascular remodelingPreeclampsiaVEGF expressionExcess thrombinAngiogenic effectsMatrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells1
Lockwood CJ, Oner C, Uz YH, Kayisli UA, Huang SJ, Buchwalder LF, Murk W, Funai EF, Schatz F. Matrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells1. Biology Of Reproduction 2008, 78: 1064-1072. PMID: 18276934, PMCID: PMC3045968, DOI: 10.1095/biolreprod.107.063743.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCase-Control StudiesCells, CulturedDeciduaDNA PrimersEnzyme InductionFemaleHumansImmunohistochemistryInterleukin-1betaMatrix Metalloproteinase 2Matrix Metalloproteinase 9Pre-EclampsiaPregnancyPregnancy Trimester, FirstReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTissue Inhibitor of Metalloproteinase-1Tissue Inhibitor of Metalloproteinase-2Tumor Necrosis Factor-alphaConceptsTumor necrosis factor alphaNecrosis factor alphaInterleukin-1 betaMedroxyprogesterone acetateFactor alphaDecidual cellsMMP9 levelsExtravillous trophoblastsEVT invasionFirst trimester decidual cellsAge-matched control womenMatrix metalloproteinase-9 expressionUteroplacental blood flowLevels of MMP9Metalloproteinase-9 expressionMatrix metalloproteinases 1Matrix metalloproteinases 2Levels of MMP2Decidual extracellular matrixConcentration-dependent increaseSubstrate gel zymographyPreeclamptic deciduaQuantitative RT-PCRInterstitial trophoblastSpiral arteries
2003
Intrauterine transfusion for rhesus alloimmunization elevates fetal β2-microglobulin levels
Radunovic N, Kuczynski E, Funai E, Nastic D, Di Renzo G, Lockwood C. Intrauterine transfusion for rhesus alloimmunization elevates fetal β2-microglobulin levels. The Journal Of Maternal-Fetal & Neonatal Medicine 2003, 13: 246-249. PMID: 12854925, DOI: 10.1080/jmf.13.4.246.249.Peer-Reviewed Original ResearchConceptsBeta2-microglobulin levelsEffects of intrauterine transfusionNon-anemic controlsIntrauterine transfusionBeta2-microglobulinTransfused fetusesCirculating T-cell populationsNon-anemic fetusesUmbilical venous samplesT cell populationsConcentration of beta2-microglobulinOrgan transplant rejectionHost immune responseRhesus alloimmunizationAnemic fetusesFetal anemiaFetal hematocritHealthy neonatesLeukocyte antigenTransplant rejectionVenous samplingTransfusionFetusesImmunomodulatory effectsImmune response
2000
Progestin-Epidermal Growth Factor Regulation of Tissue Factor Expression during Decidualization of Human Endometrial Stromal Cells
Lockwood C. Progestin-Epidermal Growth Factor Regulation of Tissue Factor Expression during Decidualization of Human Endometrial Stromal Cells. The Journal Of Clinical Endocrinology & Metabolism 2000, 85: 297-301. DOI: 10.1210/jc.85.1.297.Peer-Reviewed Original Research
1998
Matrix Metalloproteinase and Matrix Metalloproteinase Inhibitor Expression in Endometrial Stromal Cells during Progestin-Initiated Decidualization and Menstruation-Related Progestin Withdrawal**This work was supported in part by grants from the National Institutes of Health R29-HD29540–01A1 and HD-94–23 (to C.J.L.). Portions of this work were presented at the 44th Annual Meeting of the Society for Gynecologic Investigation. (Lockwood CJ, Krikun G, Hausknecht V, Wang E-Y, Ziegler D, Schatz F, Effects of RU 486 on endometrial stromal cell matrix metalloproteinase and matrix metalloproteinase inhibitor expression. Proceedings of the 44th Annual Meeting of the Society of Gynecologic Investigation, San Diego, CA, 1997, Abstract 220.
Lockwood C, Krikun G, Hausknecht V, Papp C, Schatz F. Matrix Metalloproteinase and Matrix Metalloproteinase Inhibitor Expression in Endometrial Stromal Cells during Progestin-Initiated Decidualization and Menstruation-Related Progestin Withdrawal**This work was supported in part by grants from the National Institutes of Health R29-HD29540–01A1 and HD-94–23 (to C.J.L.). Portions of this work were presented at the 44th Annual Meeting of the Society for Gynecologic Investigation. (Lockwood CJ, Krikun G, Hausknecht V, Wang E-Y, Ziegler D, Schatz F, Effects of RU 486 on endometrial stromal cell matrix metalloproteinase and matrix metalloproteinase inhibitor expression. Proceedings of the 44th Annual Meeting of the Society of Gynecologic Investigation, San Diego, CA, 1997, Abstract 220. Endocrinology 1998, 139: 4607-4613. DOI: 10.1210/en.139.11.4607.Peer-Reviewed Original ResearchSociety for Gynecologic InvestigationExpression of MMP-1Endometrial stromal cellsMMP-1 expressionGynecological investigationSteroid withdrawalImplanting trophoblastEndovascular invasionInhibitor expressionStromal cellsLocal hemorrhageMatrix metalloproteinasesMMP-1Enhanced expressionInhibition of MMP-1 expressionNational InstituteThe effect of fetal intravascular blood transfusion on plasma endothelin levels in fetuses with rhesus alloimmunization.
Radunovic N, Nastic D, Rebarber A, Kuczynski E, Lockwood C. The effect of fetal intravascular blood transfusion on plasma endothelin levels in fetuses with rhesus alloimmunization. Obstetrics And Gynecology 1998, 91: 956-60. PMID: 9611003, DOI: 10.1016/s0029-7844(98)00073-8.Peer-Reviewed Original ResearchConceptsEndothelin levelsFetal transfusionPost-transfusionTransfusion of packed red blood cellsFetal umbilical venous bloodVolume of transfused bloodPacked red blood cellsAlloimmune hemolytic anemiaIntravascular blood transfusionIntravascular fetal transfusionPost-transfusion hematocritPost-transfusion increaseUmbilical vein pressureUmbilical venous pressureUmbilical venous bloodPlasma endothelin levelsPost-transfusion measurementsRhesus alloimmunizationGestational ageBlood transfusionIntravascular volumeRed blood cellsEndothelin concentrationsHemolytic anemiaTransfusionApoptosis and Fas Expression in Human Fetal Membranes1
Runić R, Lockwood C, LaChapelle L, Dipasquale B, Demopoulos R, Kumar A, Guller S. Apoptosis and Fas Expression in Human Fetal Membranes1. The Journal Of Clinical Endocrinology & Metabolism 1998, 83: 660-666. PMID: 9467589, DOI: 10.1210/jcem.83.2.4600.Peer-Reviewed Original ResearchConceptsChorionic trophoblastsHuman fetal membranesFetal membranesApoptotic indexWeeks groupCell layerHuman fetal membrane cellsCytoplasmic domain of FasFetal membrane cellsFas messenger ribonucleic acidTrophoblast cell layerDecidual cell typesCondensation of chromatinConsistent with apoptosisSamples of amnionEpithelial cell layerMessenger ribonucleic acidCell surface receptorsWeeks gestationDecidual cellsProstate glandAmnion epitheliumFas signalingAmniotic tissueImmunohistochemical staining
1996
Expression of 11 beta-hydroxysteroid dehydrogenase during decidualization of human endometrial stromal cells
Arcuri F, Monder C, Lockwood C, Schatz F. Expression of 11 beta-hydroxysteroid dehydrogenase during decidualization of human endometrial stromal cells. Endocrinology 1996, 137: 595-600. DOI: 10.1210/en.137.2.595.Peer-Reviewed Original ResearchBeta-HSD expressionBeta-HSD activitySensitivity of mRNA detectionLow-abundance messageHSD activityType I mRNABeta-hydroxysteroid dehydrogenaseI mRNANAD(+)-dependentNADP(+)-dependentSteady state levelsDecidualization of human endometrial stromal cellsRegulates extracellular matrix turnoverNorthern analysisAbundant messageCultured stromal cellsHuman endometrial stromal cellsMedroxyprogesterone acetateBeta-hydroxysteroidEndometrial stromal cellsStromal cellsEnzyme activityTime course measurementsEarly eventExtracellular matrix turnover