1997
The Activity of the Epithelial Sodium Channel Is Regulated by Clathrin-mediated Endocytosis*
Shimkets R, Lifton R, Canessa C. The Activity of the Epithelial Sodium Channel Is Regulated by Clathrin-mediated Endocytosis*. Journal Of Biological Chemistry 1997, 272: 25537-25541. PMID: 9325269, DOI: 10.1074/jbc.272.41.25537.Peer-Reviewed Original ResearchConceptsWild-type channelsDynamin mutantPlasma membraneEpithelial sodium channelClathrin-coated pit-mediated endocytosisLiddle mutationClathrin-coated pit pathwayDominant-negative dynamin mutantPit-mediated endocytosisChannel activityLoss of endocytosisActivity of channelsLiddle's syndromePy domainsCarboxyl terminusSodium channelsWild-type ENaCBrefeldin APit pathwayEndocytosisCell surfaceSpecific inhibitorXenopus oocytesNormal turnoverMutationsDiversity of Channels Generated by Different Combinations of Epithelial Sodium Channel Subunits
McNicholas C, Canessa C. Diversity of Channels Generated by Different Combinations of Epithelial Sodium Channel Subunits. The Journal Of General Physiology 1997, 109: 681-692. PMID: 9222895, PMCID: PMC2217047, DOI: 10.1085/jgp.109.6.681.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsEpitheliumIon TransportOocytesPatch-Clamp TechniquesSodium ChannelsXenopusNoncoordinated expression of alpha-, beta-, and gamma-subunit mRNAs of epithelial Na+ channel along rat respiratory tract
Farman N, Talbot C, Boucher R, Fay M, Canessa C, Rossier B, Bonvalet J. Noncoordinated expression of alpha-, beta-, and gamma-subunit mRNAs of epithelial Na+ channel along rat respiratory tract. American Journal Of Physiology 1997, 272: c131-c141. PMID: 9038819, DOI: 10.1152/ajpcell.1997.272.1.c131.Peer-Reviewed Original ResearchConceptsRespiratory tractRat respiratory tractEpithelial cellsUpper airway epithelial cellsAirway epithelial cellsRespiratory epithelial cellsGamma-subunit mRNAType II pneumocytesRenal collecting tubulesBronchiolar epitheliumDistal colonCollecting tubuleRat lungSubunit expressionAlveolar cellsLarge prevalenceTracheal epitheliumGland ductsCellular expressionEpithelial surfaceMRNA levelsRespiratory physiologyGland aciniTractLuminal surface
1995
Expression cloning of the epithelial sodium channel
Canessa C, Horisberger J, Schild L, Rossier B. Expression cloning of the epithelial sodium channel. Kidney International 1995, 48: 950-955. PMID: 8569104, DOI: 10.1038/ki.1995.376.Peer-Reviewed Original ResearchCloning of a bovine renal epithelial Na+ channel subunit
Fuller C, Awayda M, Arrate M, Bradford A, Morris R, Canessa C, Rossier B, Benos D. Cloning of a bovine renal epithelial Na+ channel subunit. American Journal Of Physiology 1995, 269: c641-c654. PMID: 7573394, DOI: 10.1152/ajpcell.1995.269.3.c641.Peer-Reviewed Original ResearchConceptsChannel subunitsOpen reading frameProtein kinase A.Gamma-ENaC subunitsCDNA expression libraryProtein kinase CVitro translationCDNA clonesBovine cDNAXenopus laevis oocytesPancreatic microsomesAcid proteinReading frameNovel isoformBovine homologueExpression libraryConsensus sequenceKinase CBase pairsAlpha-hENaCXenopus oocytesChimeric channelsHuman counterpartLaevis oocytesSubunitsLung epithelial Na channel subunits are differentially regulated during development and by steroids
Tchepichev S, Ueda J, Canessa C, Rossier B, O'Brodovich H. Lung epithelial Na channel subunits are differentially regulated during development and by steroids. American Journal Of Physiology 1995, 269: c805-c812. PMID: 7573414, DOI: 10.1152/ajpcell.1995.269.3.c805.Peer-Reviewed Original ResearchConceptsThyroid-releasing hormoneDays gestationFirst weekGamma-rENaC mRNANormal rat fetusesAlpha-rENaCMRNA levelsNa channelsNormal rat pupsEpithelial Na channelLate fetal gestationExpression of alphaCorticosteroid therapyCorticosteroid resistanceLung's abilityPregnant ratsRat pupsAdult ratsFetal lungRat fetusesPostnatal patternGlucocorticosteroid levelsFetal gestationGestationExpression correlatesThe highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells
Puoti A, May A, Canessa C, Horisberger J, Schild L, Rossier B. The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells. American Journal Of Physiology 1995, 269: c188-c197. PMID: 7631745, DOI: 10.1152/ajpcell.1995.269.1.c188.Peer-Reviewed Original ResearchA mutation in the epithelial sodium channel causing Liddle disease increases channel activity in the Xenopus laevis oocyte expression system.
Schild L, Canessa C, Shimkets R, Gautschi I, Lifton R, Rossier B. A mutation in the epithelial sodium channel causing Liddle disease increases channel activity in the Xenopus laevis oocyte expression system. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 5699-5703. PMID: 7777572, PMCID: PMC41764, DOI: 10.1073/pnas.92.12.5699.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsEpitheliumHypertensionMutationOocytesSequence DeletionSodium ChannelsXenopus laevisConceptsLiddle's diseaseSalt-sensitive hypertensionSalt-sensitive formsChannel activityXenopus laevis oocyte expression systemDirect physiological evidenceChannel beta subunitsEpithelial sodium channelChannel hyperactivityOocyte expression systemPharmacological propertiesSodium channelsGamma subunitsMolecular targetsBeta subunitDiseaseXenopus laevis oocytesHypertensionPremature stop codonPhysiological evidenceHeritable formTruncation mutationsOverall channel activityFunctional consequencesLaevis oocytes
1994
SCNN1, an Epithelial Cell Sodium Channel Gene in the Conserved Linkage Group on Mouse Chromosome 6 and Human Chromosome 12
Meisler M, Barrow L, Canessa C, Rossier B. SCNN1, an Epithelial Cell Sodium Channel Gene in the Conserved Linkage Group on Mouse Chromosome 6 and Human Chromosome 12. Genomics 1994, 24: 185-186. PMID: 7896277, DOI: 10.1006/geno.1994.1599.Peer-Reviewed Original ResearchAn SH3 binding region in the epithelial Na+ channel (alpha rENaC) mediates its localization at the apical membrane.
Rotin D, Bar‐Sagi D, O'Brodovich H, Merilainen J, Lehto V, Canessa C, Rossier B, Downey G. An SH3 binding region in the epithelial Na+ channel (alpha rENaC) mediates its localization at the apical membrane. The EMBO Journal 1994, 13: 4440-4450. PMID: 7925286, PMCID: PMC395375, DOI: 10.1002/j.1460-2075.1994.tb06766.x.Peer-Reviewed Original ResearchConceptsC-terminal regionAlpha-spectrinSH3 domainCytoskeletal interactionsFusion proteinTerminal proline-rich regionAlpha-rENaCApical membranePolarized epithelial cellsProline-rich sequenceN-terminal proteinProline-rich regionEpithelial cellsApical membrane localizationCytoskeletal protein ankyrinProper channel functionPrimary rat alveolar epithelial cellsEpithelial cell lysatesMembrane localizationRat alveolar epithelial cellsProtein ankyrinApical localizationPlasma membraneRecombinant fusion proteinMolecular mechanismsEpithelial sodium channels
Rossier B, Canessa C, Schild L, Horisberger J. Epithelial sodium channels. Current Opinion In Nephrology & Hypertension 1994, 3: 487-496. PMID: 7804746, DOI: 10.1097/00041552-199409000-00003.Peer-Reviewed Original ResearchConceptsEpithelial sodium channelAmiloride-sensitive epithelial sodium channelSodium channelsNovel genesHeteromultimeric proteinsHomologous subunitsDistinct functionsRat epithelial sodium channelPrimary structureCation channelsBiophysical propertiesCritical roleTaste transductionTransductionGenesMechanotransductionSubunitsProteinCell distributionRegulationAmiloride-sensitive epithelial Na+ channel is made of three homologous subunits
Canessa C, Schild L, Buell G, Thorens B, Gautschi I, Horisberger J, Rossier B. Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits. Nature 1994, 367: 463-467. PMID: 8107805, DOI: 10.1038/367463a0.Peer-Reviewed Original ResearchConceptsEpithelial sodium channelRat epithelial sodium channelSodium channelsSodium reabsorptionSodium balanceDistal colonAmiloride-sensitive epithelial sodium channelPharmacological profileBlood volumeRenal tubulesRat epithelialExocrine glandsEpithelial cellsΑ-subunitDistal partNative channelsExpression cloningAldosteroneLungColon