Automation of Nanoscale Microcapillary Liquid Chromatography−Tandem Mass Spectrometry with a Vented Column
Licklider L, Thoreen C, Peng J, Gygi S. Automation of Nanoscale Microcapillary Liquid Chromatography−Tandem Mass Spectrometry with a Vented Column. Analytical Chemistry 2002, 74: 3076-3083. PMID: 12141667, DOI: 10.1021/ac025529o.Peer-Reviewed Original ResearchConceptsPeptide mixturesSample introduction stepComplex peptide mixturesTandem MS experimentsMS analysisReversed phase LC-MS/MS analysisLiquid chromatography-tandem mass spectrometry analysisColumn enrichmentPeptide samplesElectrospray sourceMass spectrometry analysisMS experimentsCapillary columnLarge sample volumesMass spectrometryLC-MS/MS analysisColumn washingIntroduction stepChromatography fractionsLine separationSpectrometry analysisNano-LCLoop injectorSample volumeColumn