2021
Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo
Vinter D, Hoppe C, Ashe H. Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo. STAR Protocols 2021, 2: 100812. PMID: 34585149, PMCID: PMC8450298, DOI: 10.1016/j.xpro.2021.100812.Peer-Reviewed Original ResearchDynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo
Vinter DJ, Hoppe C, Minchington TG, Sutcliffe C, Ashe HL. Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. Development 2021, 148: dev196121. PMID: 33722899, PMCID: PMC8077512, DOI: 10.1242/dev.196121.Peer-Reviewed Original ResearchConceptsSingle mRNA resolutionDrosophila embryosHb mRNAMRNA translationAnterior-posterior patterningLive embryosSingle-molecule imagingTissue culture cellsTranslational repressionDevelopmental patterningSpatiotemporal regulationTranslational regulationExpression domainsSunTag systemTranscription factorsExpression boundariesTranslation dynamicsEmbryosCulture cellsMRNARepressionRegulationPatterningDrosophilaTranslation
2020
Modulation of the Promoter Activation Rate Dictates the Transcriptional Response to Graded BMP Signaling Levels in the Drosophila Embryo
Hoppe C, Bowles JR, Minchington TG, Sutcliffe C, Upadhyai P, Rattray M, Ashe HL. Modulation of the Promoter Activation Rate Dictates the Transcriptional Response to Graded BMP Signaling Levels in the Drosophila Embryo. Developmental Cell 2020, 54: 727-741.e7. PMID: 32758422, PMCID: PMC7527239, DOI: 10.1016/j.devcel.2020.07.007.Peer-Reviewed Original ResearchConceptsDrosophila embryosCell fate decisionsMorphogen gradient interpretationTarget gene transcriptsSingle-cell resolutionBMP gradientMRNA outputAxis patterningCell fateExpression domainsTranscriptional responsePromoter sequencesGene transcriptsProtein gradientGradient interpretationBurst kineticsBMPEmbryosEnhancerClassic exampleCellsActivation rateMorphogensTranscriptsGeneral features