Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo
Vinter D, Hoppe C, Ashe H. Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo. STAR Protocols 2021, 2: 100812. PMID: 34585149, PMCID: PMC8450298, DOI: 10.1016/j.xpro.2021.100812.Peer-Reviewed Original ResearchDynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo
Vinter DJ, Hoppe C, Minchington TG, Sutcliffe C, Ashe HL. Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. Development 2021, 148: dev196121. PMID: 33722899, PMCID: PMC8077512, DOI: 10.1242/dev.196121.Peer-Reviewed Original ResearchConceptsSingle mRNA resolutionDrosophila embryosHb mRNAMRNA translationAnterior-posterior patterningLive embryosSingle-molecule imagingTissue culture cellsTranslational repressionDevelopmental patterningSpatiotemporal regulationTranslational regulationExpression domainsSunTag systemTranscription factorsExpression boundariesTranslation dynamicsEmbryosCulture cellsMRNARepressionRegulationPatterningDrosophilaTranslation