Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo
Vinter DJ, Hoppe C, Minchington TG, Sutcliffe C, Ashe HL. Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. Development 2021, 148: dev196121. PMID: 33722899, PMCID: PMC8077512, DOI: 10.1242/dev.196121.Peer-Reviewed Original ResearchConceptsSingle mRNA resolutionDrosophila embryosHb mRNAMRNA translationAnterior-posterior patterningLive embryosSingle-molecule imagingTissue culture cellsTranslational repressionDevelopmental patterningSpatiotemporal regulationTranslational regulationExpression domainsSunTag systemTranscription factorsExpression boundariesTranslation dynamicsEmbryosCulture cellsMRNARepressionRegulationPatterningDrosophilaTranslationCRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
Hoppe C, Ashe HL. CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos. STAR Protocols 2021, 2: 100380. PMID: 33786461, PMCID: PMC7988240, DOI: 10.1016/j.xpro.2021.100380.Peer-Reviewed Original Research