2010
Sin1-mTORC2 Suppresses rag and il7r Gene Expression through Akt2 in B Cells
Lazorchak AS, Liu D, Facchinetti V, Di Lorenzo A, Sessa WC, Schatz DG, Su B. Sin1-mTORC2 Suppresses rag and il7r Gene Expression through Akt2 in B Cells. Molecular Cell 2010, 39: 433-443. PMID: 20705244, PMCID: PMC2957800, DOI: 10.1016/j.molcel.2010.07.031.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsB-LymphocytesCell Line, TransformedDNA-Binding ProteinsForkhead Box Protein O1Forkhead Transcription FactorsGene Expression RegulationGene Rearrangement, B-LymphocyteHomeodomain ProteinsMiceMice, KnockoutPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReceptors, Interleukin-7Signal TransductionTOR Serine-Threonine KinasesTranscription FactorsConceptsB cell developmentGene expressionCell developmentRAG gene expressionMTOR complex 2FOXO1 transcriptional activityPI3K signalingMTOR inhibitor rapamycinTranscriptional activityKey regulatorB cellsMolecular mechanismsInhibitor rapamycinK signalingCell survivalFoxO1 phosphorylationMammalian targetRecombinase activityPI3KIL-7 receptorAkt2SignalingRapamycinExpressionCells
2008
The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C
Facchinetti V, Ouyang W, Wei H, Soto N, Lazorchak A, Gould C, Lowry C, Newton AC, Mao Y, Miao RQ, Sessa WC, Qin J, Zhang P, Su B, Jacinto E. The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C. The EMBO Journal 2008, 27: 1932-1943. PMID: 18566586, PMCID: PMC2486276, DOI: 10.1038/emboj.2008.120.Peer-Reviewed Original ResearchConceptsTarget of rapamycinProtein kinase CTOR complex 1Functions of TORRapamycin-sensitive TOR complex 1Site phosphorylationKinase CTurn motif siteProtein synthesisStability of AktConventional protein kinase CTORC2 functionTOR functionTOR pathwayPhosphorylation of AktHM siteKinase domainProtein foldingMotif sitesProteasome degradationNovel functionBasic residuesMammalian targetPhosphorylationAkt
2006
SIN1/MIP1 Maintains rictor-mTOR Complex Integrity and Regulates Akt Phosphorylation and Substrate Specificity
Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J, Su B. SIN1/MIP1 Maintains rictor-mTOR Complex Integrity and Regulates Akt Phosphorylation and Substrate Specificity. Cell 2006, 127: 125-137. PMID: 16962653, DOI: 10.1016/j.cell.2006.08.033.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCells, CulturedFibroblastsForkhead Box Protein O1Forkhead Box Protein O3Forkhead Transcription FactorsGene SilencingHumansMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesPhosphoproteinsPhosphorylationProtein KinasesProteinsProto-Oncogene Proteins c-aktRapamycin-Insensitive Companion of mTOR ProteinRegulatory-Associated Protein of mTORSignal TransductionSubstrate SpecificityThreonineTOR Serine-Threonine KinasesTranscription FactorsConceptsAkt Ser473 phosphorylationAkt targetsSer473 phosphorylationAkt/PKB Ser473 phosphorylationPKB Ser473 phosphorylationRecent biochemical studiesTORC2 functionTORC1 functionHydrophobic motifProtein complexesRaptor-mTORMTOR functionActivation loopPhosphorylation sitesAkt functionSubstrate specificityComplex integrityS6KRegulatory mechanismsCell survivalPhosphorylationMammalian targetPhysiological importanceAkt phosphorylationBiochemical studies