1996
Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney
Biemesderfer D, Payne J, Lytle C, Forbush B. Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney. American Journal Of Physiology 1996, 270: f927-f936. PMID: 8764311, DOI: 10.1152/ajprenal.1996.270.6.f927.Peer-Reviewed Original ResearchConceptsNa-K-Cl cotransporterNa-K-ClBasolateral membraneCotransporter proteinBasolateral membranes of proximal tubulesNa-K-Cl cotransport proteinShark kidneyBasolateral plasma membraneDistal tubule segmentsApical plasma membraneShark rectal glandDistal nephronReabsorptive epitheliaNaCl reabsorptionPlasma membraneWestern blot analysisTubule segmentsProximal tubulesDiluting segmentWeak stainingShark Squalus acanthiasPresence of multiple isoformsDogfish shark Squalus acanthiasCotransporterMonoclonal antibodies
1985
Monoclonal antibody to Na,K-ATPase: Immunocytochemical localization along nephron segments
Kashgarian M, Biemesderfer D, Caplan M, Forbush B. Monoclonal antibody to Na,K-ATPase: Immunocytochemical localization along nephron segments. Kidney International 1985, 28: 899-913. PMID: 3003443, DOI: 10.1038/ki.1985.216.Peer-Reviewed Original ResearchConceptsMonoclonal antibodiesNephron segmentsEvidence of labellingRenal tubular epithelial cellsTubular epithelial cellsK-ATPaseThick ascending limbOnly principal cellsDifferent nephron segmentsDog antigensBasal-lateral membranesSignificant antibodiesAscending limbMesangial regionHenle's loopOccasional cellsRenal medullaOuter renal medullaAbundant antibodyAntibodiesApical labelingTubule segmentsPrincipal cellsEpithelial cellsImmunocytochemical localization