1983
[3H]bumetanide binding to membranes isolated from dog kidney outer medulla. Relationship to the Na,K,Cl co-transport system.
Forbush B, Palfrey H. [3H]bumetanide binding to membranes isolated from dog kidney outer medulla. Relationship to the Na,K,Cl co-transport system. Journal Of Biological Chemistry 1983, 258: 11787-11792. PMID: 6619143, DOI: 10.1016/s0021-9258(17)44299-2.Peer-Reviewed Original ResearchConceptsOuter medullaCl- co-transport systemDog kidney outer medullaCl- cotransport systemLoop of HenleCo-transport systemLoop diureticsBasolateral membraneKidney outer medullaProximal tubulesAscending limbLuminal membraneCotransport systemKidney cortexMedullaKidney membranesBrush borderCo-transportCrude membranesKidneyDog kidneyBinding to membranesBinding activityGradient centrifugationNative Membranes from Dog Kidney Outer Medulla, Enriched in Na,K-ATPase and Vesicular in Nature
Forbush B. Native Membranes from Dog Kidney Outer Medulla, Enriched in Na,K-ATPase and Vesicular in Nature. Current Topics In Membranes 1983, 19: 113-118. DOI: 10.1016/s0070-2161(08)60556-0.Peer-Reviewed Original ResearchDog kidney outer medullaNa,K-ATPaseOuter medullaKidney outer medullaK-ATPaseNa,K-ATPase activityActivity of Na,K-ATPaseK-ATPase activityMembrane vesiclesSucrose gradient centrifugationMedullaNative membranesMammalian kidneyIncreased permeabilityCrude microsomal fractionGradient centrifugationCentrifugation mediumMicrosomal membranes
1982
Characterization of right-side-out membrane vesicles rich in (Na,K)-ATPase and isolated from dog kidney outer medulla.
Forbush B. Characterization of right-side-out membrane vesicles rich in (Na,K)-ATPase and isolated from dog kidney outer medulla. Journal Of Biological Chemistry 1982, 257: 12678-12684. PMID: 6290476, DOI: 10.1016/s0021-9258(18)33564-6.Peer-Reviewed Original ResearchConceptsDog kidney outer medullaK)-ATPase activityK)-ATPaseKidney outer medullaOuter medullaRight-side-out membrane vesiclesMembrane vesiclesBasolateral membranePorcine trypsinMg2+ + ATPRight-side-out orientationDensity gradient centrifugationCaged ATPIntravesicular volumePresence of Na+H1 populationMedullaMembrane proteinsSucrose gradientsGradient centrifugationTrypsin-sensitiveDetergent treatmentVesicle volumeVesiclesATP