2022
The Effect of Tobacco Smoke N-Nitrosamines, NNK and NDEA, and Nicotine, on DNA Mismatch Repair Mechanism and miRNA Markers, in Hypopharyngeal Squamous Cell Carcinoma: An In Vivo Model and Clinical Evidence
Doukas SG, Vageli DP, Doukas PG, Nikitovic D, Tsatsakis A, Judson BL. The Effect of Tobacco Smoke N-Nitrosamines, NNK and NDEA, and Nicotine, on DNA Mismatch Repair Mechanism and miRNA Markers, in Hypopharyngeal Squamous Cell Carcinoma: An In Vivo Model and Clinical Evidence. Current Oncology 2022, 29: 5531-5549. PMID: 36005175, PMCID: PMC9406897, DOI: 10.3390/curroncol29080437.Peer-Reviewed Original ResearchConceptsHypopharyngeal squamous cell carcinomaSquamous cell carcinomaClinical evidenceCell carcinomaTobacco smokeChronic exposureUpper aerodigestive tract cancerHuman hypopharyngeal squamous cell carcinomaAerodigestive tract cancerUpper aerodigestive tract carcinogenesisAerodigestive tract carcinogenesisDose-dependent effectDNA mismatch repair mechanismHypopharyngeal cellsTract cancerTobacco smokersPoor prognosisHypopharyngeal mucosaMouse modelClinical testingVivo modelMiR-155MiR-451aVivo dose-dependent effectMismatch repair mechanismThe Role of PARP‐1 and NF‐κB in Bile‐Induced DNA Damage and Oncogenic Profile in Hypopharyngeal Cells
Doukas PG, Vageli DP, Judson BL. The Role of PARP‐1 and NF‐κB in Bile‐Induced DNA Damage and Oncogenic Profile in Hypopharyngeal Cells. The Laryngoscope 2022, 133: 1146-1155. PMID: 35791892, DOI: 10.1002/lary.30284.Peer-Reviewed Original ResearchConceptsHypopharyngeal cellsAcidic bileNF-κBNF-κB activationGamma-H2A histone family member XHypopharyngeal squamous cell carcinomaOncogenic profileNuclear factor kappa BPARP-1Squamous cell carcinomaB-cell lymphoma 2 expressionNF-κB transcriptional activityFactor kappa BDNA damageEnzyme-linked immunosorbent assayH2A histone family member XCell survivalDNA/RNA damageHypopharyngeal carcinogenesisReflux contentsAnti-apoptotic phenotypeCell carcinomaWidespread DNA damageBAY 11PARP-1 overexpression
2021
Targeting STAT3 prevents bile reflux‐induced oncogenic molecular events linked to hypopharyngeal carcinogenesis
Vageli DP, Doukas PG, Siametis A, Judson BL. Targeting STAT3 prevents bile reflux‐induced oncogenic molecular events linked to hypopharyngeal carcinogenesis. Journal Of Cellular And Molecular Medicine 2021, 26: 75-87. PMID: 34850540, PMCID: PMC8742186, DOI: 10.1111/jcmm.17011.Peer-Reviewed Original ResearchConceptsBcl-2 overexpressionTranscriptional activationTranscription factorsTranscriptional activitySignal transducerSTAT3 knockdownMolecular eventsCell survivalQPCR analysisLuciferase assaySTAT3 activationTargeting STAT3Oncogenic eventsAberrant activationHypopharyngeal carcinogenesisSTAT3 phosphorylationSTAT3Oncogenic effectsSTAT3 pathwayPharmacologic inhibitionPharmacological inhibitionSTA-21Hypopharyngeal cellsOncogenic profileWestern blotPepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells
Doukas PG, Vageli DP, Sasaki CT, Judson BL. Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells. International Journal Of Molecular Sciences 2021, 22: 4275. PMID: 33924087, PMCID: PMC8074291, DOI: 10.3390/ijms22084275.Peer-Reviewed Original ResearchConceptsRelated-signaling pathwaysOncogenic pathwaysLaryngopharyngeal reflux diseaseEpidermal growth factor receptorSpecific oncogenic pathwaysDownstream oncogenic pathwaysGrowth factor receptorHypopharyngeal cellsReflux diseaseRisk factorsTherapeutic approachesIntermittent exposureTumorigenic effectsPronounced activationOncogenic factorSignificant upregulationFactor receptorSTAT3 activationAcidic pepsinEGFR activationCell viabilityPromotes ActivationActivationPrimary cellsCells