2011
Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
Abe R, Beckett J, Abe R, Nixon A, Rochier A, Yamashita N, Sumpio B. Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation. European Journal Of Vascular And Endovascular Surgery 2011, 41: 814-820. PMID: 21333557, DOI: 10.1016/j.ejvs.2010.12.021.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationCell cycle analysisSMC proliferationCell proliferationInhibits Smooth Muscle Cell ProliferationVascular smooth muscle cell proliferationCoronary artery diseaseNon-treated groupExtracellular signal-regulated kinase 1/2 activationCardiovascular mortalityArtery diseaseKinase 1/2 activationCell cycle blockG1-S phaseMediterranean dietDay 1Oleuropein treatmentGrowth of SMCsG1 phase regulatorsFlow cytometryVascular SMCsPresence of oleuropeinBeneficial effectsCycle analysis
2009
Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1
Esemuede N, Lee T, Maier KG, Sumpio BE, Gahtan V. Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1. Journal Of Surgical Research 2009, 168: 149-154. PMID: 20338582, DOI: 10.1016/j.jss.2009.11.728.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell ProliferationCells, CulturedChemotaxisDose-Response Relationship, DrugHydroxymethylglutaryl-CoA Reductase InhibitorsLovastatinMevalonic AcidModels, AnimalMonomeric GTP-Binding ProteinsMuscle, Smooth, VascularRas ProteinsSignal TransductionThrombospondin 1Tunica IntimaTunica MediaConceptsTSP-1-induced chemotaxisGeranylgeranyl transferase inhibitorSerum-free mediumThrombospondin-1HMG-CoA reductase inhibitorsVascular smooth muscle cell migrationSmooth muscle cell migrationTransferase inhibitorsEffect of lovastatinG proteinsMuscle cell migrationRas activationRho-kinase inhibitorCell migrationPost-hoc testingFarnesyl transferase inhibitorsCholesterol loweringLovastatin doseVascular restenosisIntimal hyperplasiaBoyden chamberPleiotropic propertiesReductase inhibitorsWestern blotInhibition of Ras
2008
The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival. Journal Of Surgical Research 2008, 155: 125-131. PMID: 19059608, DOI: 10.1016/j.jss.2008.07.029.Peer-Reviewed Original ResearchConceptsERK 1/2P38 MAPKProtein kinaseKeratinocyte proliferation rateCell deathAkt inhibitorCyclic stretchDNA synthesisAktTransferase-mediated dUTP nick-end labeling stainingRepetitive stretchApoptosisMAPKDUTP nick end labeling stainingProliferation rateHuman dermalCell numberNick end labeling stainingTUNEL-positive cellsDermal keratinocytesStretchTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingBrdU stainingKeratinocyte proliferationProliferationSynergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty
Yiu WK, Cheng SW, Sumpio BE. Synergistic Effect of Cool/Thaw Cycles on Vascular Cells in an In Vitro Model of Cryoplasty. Journal Of Vascular And Interventional Radiology 2008, 19: 925-930. PMID: 18503909, DOI: 10.1016/j.jvir.2008.02.007.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAkt activationEndothelial cellsApoptotic smooth muscle cellsActivation of AktBovine aortic smooth muscle cellsAortic smooth muscle cellsSurvival responseDeoxynucleotidyl transferase-mediated dUTP nick end labelingFetal bovine serumTerminal deoxynucleotidyl transferase-mediated dUTP nick end labelingTransferase-mediated dUTP nick end labelingImmunoblot analysisDUTP nick end labelingHigher apoptotic rateVascular cells
2007
Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival. Wound Repair And Regeneration 2007, 15: 646-656. PMID: 17971010, DOI: 10.1111/j.1524-475x.2007.00295.x.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMAPK/ERK kinaseExtracellular signal-regulated kinaseDominant-negative AktHuman dermal fibroblastsSignal-regulated kinaseCell numberERK kinaseProtein kinaseTransduction pathwaysDermal fibroblastsRelevant transduction pathwaysRelevant signal pathwaysDermal fibroblast proliferationHuman dermal fibroblast proliferationCultured human dermal fibroblastsSurvival of fibroblastsSignal pathwayKinaseNecrotic fibroblastsDNA synthesisCell proliferationAktTransferase-mediated dUTP nick-end labeling stainingDirect comparison of endothelial cell and smooth muscle cell response to supercooling and rewarming
Yiu WK, Cheng SW, Sumpio BE. Direct comparison of endothelial cell and smooth muscle cell response to supercooling and rewarming. Journal Of Vascular Surgery 2007, 46: 557-564.e2. PMID: 17826245, DOI: 10.1016/j.jvs.2007.04.072.Peer-Reviewed Original ResearchAnimalsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedCryopreservationDensitometryEndothelial CellsEnzyme ActivationIn Situ Nick-End LabelingMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle, Smooth, VascularPhosphorylationProto-Oncogene Proteins c-aktRewarmingEffects of different types of fluid shear stress on endothelial cell proliferation and survival
Kadohama T, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. Effects of different types of fluid shear stress on endothelial cell proliferation and survival. Journal Of Cellular Physiology 2007, 212: 244-251. PMID: 17323381, DOI: 10.1002/jcp.21024.Peer-Reviewed Original ResearchPhosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTEN
2006
Green Tea, the “Asian Paradox,” and Cardiovascular Disease
Sumpio BE, Cordova AC, Berke-Schlessel DW, Qin F, Chen QH. Green Tea, the “Asian Paradox,” and Cardiovascular Disease. Journal Of The American College Of Surgeons 2006, 202: 813-825. PMID: 16648021, DOI: 10.1016/j.jamcollsurg.2006.01.018.Peer-Reviewed Original ResearchRole of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylation
2005
Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis
Poussier B, Cordova AC, Becquemin JP, Sumpio BE. Resveratrol inhibits vascular smooth muscle cell proliferation and induces apoptosis. Journal Of Vascular Surgery 2005, 42: 1190-1190.e14. PMID: 16376213, DOI: 10.1016/j.jvs.2005.08.014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis InhibitorsAnimalsAnimals, NewbornAntioxidantsAorta, ThoracicApoptosisBlotting, WesternCattleCell ProliferationCells, CulturedDNADose-Response Relationship, DrugFlow CytometryIn Situ Nick-End LabelingIn Vitro TechniquesMuscle, Smooth, VascularProliferating Cell Nuclear AntigenResveratrolStilbenesConceptsSmooth muscle cell proliferationMuscle cell proliferationVascular smooth muscle cell proliferationDose-dependent mannerSMC proliferationCell proliferationG1-S phaseBeneficial effectsFrench paradoxDose-dependent apoptotic effectTerminal deoxynucleotidyl transferase-mediated dUTP-biotin nickTransferase-mediated dUTP-biotin nickCardiovascular death ratesRed wine intakeRed wine consumptionEffects of resveratrolAortic SMC proliferationEnd labeling stainingVascular SMC proliferationDUTP-biotin nickMajor polyphenol componentHealthy life styleCell cycle analysisCardiovascular mortalityFlow-activated cell sortingDifferential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blottingSustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway
Asada H, Paszkowiak J, Teso D, Alvi K, Thorisson A, Frattini JC, Kudo FA, Sumpio BE, Dardik A. Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway. Journal Of Vascular Surgery 2005, 42: 772-780. PMID: 16242567, DOI: 10.1016/j.jvs.2005.05.046.Peer-Reviewed Original ResearchConceptsOrbital shear stressPresence of PD98059Synthetic phenotypeERK1/2 pathwayExtracellular signal-regulated proteinSMC proliferationSmooth muscle cell proliferationMuscle cell proliferationPathway inhibitor PD98059Cell proliferationExtracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylationKinase 1/2 phosphorylationPresence of SB203580Bovine SMCsSMC phenotypeInhibitor PD98059Inhibitor SB203580Contractile phenotypePD98059Long-term culturePhenotypeCell nuclear antigenExpression of markersPathwaySB203580The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain
Kakisis JD, Pradhan S, Cordova A, Liapis CD, Sumpio BE. The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain. The International Journal Of Biochemistry & Cell Biology 2005, 37: 1396-1406. PMID: 15833272, DOI: 10.1016/j.biocel.2005.01.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCell ProliferationCells, CulturedDNA-Binding ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myocytes, Smooth MusclePhosphorylationPyrazolesPyrimidinesRatsSerineSignal TransductionSrc-Family KinasesSTAT3 Transcription FactorStress, MechanicalTrans-ActivatorsTyrosineConceptsSerine phosphorylationTyrosine phosphorylationSmooth muscle cellsSTAT-3Vascular smooth muscle cellsSTAT-3 tyrosine phosphorylationSpecific inhibitorVascular cell morphologyExtracellular signal-regulated kinase 1/2Extracellular mechanical signalsSignal-regulated kinase 1/2Basal serine phosphorylationInhibition of SrcA7r5 smooth muscle cellsNegative feedback loopInhibition of ERK1/2Cells sensePhosphospecific antibodiesPhosphatidylinositol 3Signal transducerTranscription 3Kinase 2Kinase 1/2Smooth muscle cell responseBasal phosphorylationThe cardiovascular protective effect of red wine
Cordova AC, Jackson L, Berke-Schlessel DW, Sumpio BE. The cardiovascular protective effect of red wine. Journal Of The American College Of Surgeons 2005, 200: 428-439. PMID: 15737855, DOI: 10.1016/j.jamcollsurg.2004.10.030.Peer-Reviewed Original Research
2004
Molecular and biological effects of hemodynamics on vascular cells.
Pradhan S, Sumpio B. Molecular and biological effects of hemodynamics on vascular cells. Frontiers In Bioscience-Landmark 2004, 9: 3276-85. PMID: 15353357, DOI: 10.2741/1480.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtherosclerosisCell MovementCell ProliferationCells, CulturedCyclic AMPDiglyceridesDisease ProgressionEndothelial CellsExtracellular MatrixHemodynamicsHumansInositol 1,4,5-TrisphosphateIntercellular Adhesion Molecule-1Plasminogen ActivatorsProtein Kinase CRisk FactorsStress, MechanicalConceptsEndothelial cellsSignificant atherosclerotic plaqueSystemic risk factorsTissue plasminogen activatorCellular adhesion moleculesCurrent pertinent literatureCultured endothelial cellsRisk factorsSecond messenger systemsSignificant lesionsProtein kinase C pathwayICAM-1Atherosclerosis formationAtherosclerotic plaquesVascular anatomyKinase C pathwayNitric oxideVascular cellsPlasminogen activatorAdhesion moleculesBlood vesselsVascular biologyMessenger systemsVessel wallPertinent literature