2023
Identification of Efflux Substrates Using a Riboswitch-Based Reporter in Pseudomonas aeruginosa
Urdaneta-Páez V, Hamchand R, Anthony K, Crawford J, Sutherland A, Kazmierczak B. Identification of Efflux Substrates Using a Riboswitch-Based Reporter in Pseudomonas aeruginosa. MSphere 2023, 8: e00069-23. PMID: 36946743, PMCID: PMC10117056, DOI: 10.1128/msphere.00069-23.Peer-Reviewed Original ResearchConceptsLiquid chromatography-mass spectrometryCompound uptakeHigh-resolution liquid chromatography-mass spectrometryChromatography-mass spectrometryNovel antibioticsHigh-throughput screeningRational designMore rational designChemical librariesDiverse compoundsInitial hitsSelect compoundsPermeable compoundsDrug candidatesCompoundsStructural propertiesBacterial cellsPowerful methodAntifolate drugsSubstrateSpectrometrySynthesisPseudomonas aeruginosaClasses of antibioticsMembrane
2013
Spatial and numerical regulation of flagellar biosynthesis in polarly flagellated bacteria
Kazmierczak BI, Hendrixson DR. Spatial and numerical regulation of flagellar biosynthesis in polarly flagellated bacteria. Molecular Microbiology 2013, 88: 655-663. PMID: 23600726, PMCID: PMC3654036, DOI: 10.1111/mmi.12221.Peer-Reviewed Original ResearchConceptsPolar flagellatesFlagellar assemblyProper cell divisionRotation of flagellaFlagellar biosynthesisFlagellation patternsGram-negative bacteriaPeritrichous flagellaOrganelle numberCell divisionCell biologyFlhGFlhFBacterial speciesBacterial cellsFlagellatesFlagellaDifferent bacteriaMechanistic insightsProteinBacteriaRecent findingsSpeciesSpecific activityDistinct patterns