2018
In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons
Huang T, Nguyen L, Lin TV, Gong X, Zhang L, Kim GB, Sarkisian MR, Breunig JJ, Bordey A. In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons. Neuroscience Research 2018, 143: 44-52. PMID: 29857015, PMCID: PMC6265126, DOI: 10.1016/j.neures.2018.05.006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDisks Large Homolog 4 ProteinDoublecortin ProteinElectroporationEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMiceOligonucleotide Array Sequence AnalysisProtein BiosynthesisPyramidal CellsRecombinant Fusion ProteinsRibosomal Protein L10Ribosomal ProteinsRNA, MessengerSomatosensory CortexSynapsinsConceptsSpecific neuronal populationsRibosomal protein L10aRibosome affinity purificationSelective biological processesNeuronal genesMolecular profileSpecific developmental patternsTarget genesAffinity purificationBiological processesGenesUtero electroporationNeuronal populationsEmbryonic day 14Molecular profilingDevelopmental patternsMRNA expression
2008
Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole‐mounts of the subventricular zone
Platel J, Heintz T, Young S, Gordon V, Bordey A. Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole‐mounts of the subventricular zone. The Journal Of Physiology 2008, 586: 3783-3793. PMID: 18565997, PMCID: PMC2538932, DOI: 10.1113/jphysiol.2008.155879.Peer-Reviewed Original ResearchConceptsKainate receptorsSVZ neuroblastsLateral ventricleSubventricular zoneNeuroblast migrationAcute slicesMetabotropic glutamate receptor subtype 5AMPA/kainate receptorsMGluR5 antagonist MPEPPermeable kainate receptorsReceptor antagonist bicucullineActivation of mGluR5Postnatal day 20Postnatal subventricular zoneDCX promoterAntagonist MPEPWhole-mount preparationsAntagonist bicucullineMGluR5 activationCell aspiratesReceptor antagonistMouse slicesTonic activationSubtype 5Immunopositive cells