Transient mGlu5R inhibition enhances the survival of granule cell precursors in the neonatal cerebellum
Kubera C, Hernandez AL, Heng V, Bordey A. Transient mGlu5R inhibition enhances the survival of granule cell precursors in the neonatal cerebellum. Neuroscience 2012, 219: 271-279. PMID: 22677205, PMCID: PMC3402690, DOI: 10.1016/j.neuroscience.2012.05.064.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornApoptosisCell SurvivalCerebellumFemaleIn Situ Nick-End LabelingMaleMiceNeural Stem CellsNeurogenesisReal-Time Polymerase Chain ReactionReceptor, Metabotropic Glutamate 5Receptors, Metabotropic GlutamateReverse Transcriptase Polymerase Chain ReactionConceptsExternal germinal layerGranule cell precursorsGranule cellsS-phase marker bromodeoxyuridineMetabotropic glutamate receptor 5Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stainingTransferase dUTP nick end labeling stainingCell precursorsDUTP nick end labeling stainingNick end labeling stainingGlutamate receptor 5Postnatal day 2Proliferative granule cell precursorsEnd labeling stainingCerebellar granule cellsAcute slicesAbundant neuronsBrdU injectionLabeling stainingVivo treatmentReceptor 5Day 2Vivo injectionSpecific receptorsClonal expansion