2016
Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP
Bartley CM, O’Keefe R, Blice-Baum A, Mihailescu MR, Gong X, Miyares L, Karaca E, Bordey A. Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP. ENeuro 2016, 3: eneuro.0092-16.2016. PMID: 27957526, PMCID: PMC5116651, DOI: 10.1523/eneuro.0092-16.2016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCasein Kinase IICells, CulturedCerebral CortexFragile X Mental Retardation ProteinHEK293 CellsHumansMass SpectrometryMiceNaphthyridinesPhenazinesPhosphorylationProtein BiosynthesisProtein Kinase InhibitorsReceptors, Metabotropic GlutamateRecombinant ProteinsTime FactorsConceptsTranslational repressionNearby residuesProtein phosphatase 2ACasein kinase IIMental retardation proteinFMRP lossPhosphatase 2AFragile X syndromePP2A pathwaySecondary phosphorylationProtein translationKinase IIGene transcriptsFMRPBrain transcriptsFunction mutationsPhosphorylationS499X syndromeTranscriptsRepressionResiduesRecent evidenceCK2Kinase
2012
Transient mGlu5R inhibition enhances the survival of granule cell precursors in the neonatal cerebellum
Kubera C, Hernandez AL, Heng V, Bordey A. Transient mGlu5R inhibition enhances the survival of granule cell precursors in the neonatal cerebellum. Neuroscience 2012, 219: 271-279. PMID: 22677205, PMCID: PMC3402690, DOI: 10.1016/j.neuroscience.2012.05.064.Peer-Reviewed Original ResearchConceptsExternal germinal layerGranule cell precursorsGranule cellsS-phase marker bromodeoxyuridineMetabotropic glutamate receptor 5Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stainingTransferase dUTP nick end labeling stainingCell precursorsDUTP nick end labeling stainingNick end labeling stainingGlutamate receptor 5Postnatal day 2Proliferative granule cell precursorsEnd labeling stainingCerebellar granule cellsAcute slicesAbundant neuronsBrdU injectionLabeling stainingVivo treatmentReceptor 5Day 2Vivo injectionSpecific receptorsClonal expansion