In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons
Huang T, Nguyen L, Lin TV, Gong X, Zhang L, Kim GB, Sarkisian MR, Breunig JJ, Bordey A. In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons. Neuroscience Research 2018, 143: 44-52. PMID: 29857015, PMCID: PMC6265126, DOI: 10.1016/j.neures.2018.05.006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDisks Large Homolog 4 ProteinDoublecortin ProteinElectroporationEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMiceOligonucleotide Array Sequence AnalysisProtein BiosynthesisPyramidal CellsRecombinant Fusion ProteinsRibosomal Protein L10Ribosomal ProteinsRNA, MessengerSomatosensory CortexSynapsinsConceptsSpecific neuronal populationsRibosomal protein L10aRibosome affinity purificationSelective biological processesNeuronal genesMolecular profileSpecific developmental patternsTarget genesAffinity purificationBiological processesGenesUtero electroporationNeuronal populationsEmbryonic day 14Molecular profilingDevelopmental patternsMRNA expression