2012
Preparation of acute subventricular zone slices for calcium imaging.
Lacar B, Young SZ, Platel JC, Bordey A. Preparation of acute subventricular zone slices for calcium imaging. Journal Of Visualized Experiments 2012, e4071. PMID: 23023088, PMCID: PMC3490239, DOI: 10.3791/4071.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCell typesSVZ cellsProgenitor cellsNeurogenic zonesSubventricular zoneFluo-4 AM dyeNeural progenitor cellsIntermediate progenitor cellsExtracellular signalsDiffusible signalsIntercellular signalingSVZ cell typesTime-lapse moviesCalcium indicator dyeCalcium activityIndividual cellsRostral-caudal axisNeuroblast migrationThree-dimensional arrangementAdult hippocampal subgranular zonePostnatal brainAdhesion moleculesHippocampal subgranular zoneMature astrocytesNeuroblasts
2008
Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole‐mounts of the subventricular zone
Platel J, Heintz T, Young S, Gordon V, Bordey A. Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole‐mounts of the subventricular zone. The Journal Of Physiology 2008, 586: 3783-3793. PMID: 18565997, PMCID: PMC2538932, DOI: 10.1113/jphysiol.2008.155879.Peer-Reviewed Original ResearchConceptsKainate receptorsSVZ neuroblastsLateral ventricleSubventricular zoneNeuroblast migrationAcute slicesMetabotropic glutamate receptor subtype 5AMPA/kainate receptorsMGluR5 antagonist MPEPPermeable kainate receptorsReceptor antagonist bicucullineActivation of mGluR5Postnatal day 20Postnatal subventricular zoneDCX promoterAntagonist MPEPWhole-mount preparationsAntagonist bicucullineMGluR5 activationCell aspiratesReceptor antagonistMouse slicesTonic activationSubtype 5Immunopositive cells