2014
Lipid-Dependent Bimodal MCL1 Membrane Activity
Landeta O, Valero J, Flores-Romero H, Bustillo-Zabalbeitia I, Landajuela A, Garcia-Porras M, Terrones O, Basañez G. Lipid-Dependent Bimodal MCL1 Membrane Activity. ACS Chemical Biology 2014, 9: 2852-2863. PMID: 25314294, DOI: 10.1021/cb500592e.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBcl-2-Associated X ProteinBH3 Interacting Domain Death Agonist ProteinCardiolipinsCholesterolFibroblastsGene ExpressionHeLa CellsHumansLiposomesMembrane LipidsMembrane Potential, MitochondrialMiceMitochondriaModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedMyeloid Cell Leukemia Sequence 1 ProteinPhosphatidylinositolsProtein Structure, TertiaryProto-Oncogene Proteins c-bcl-2Recombinant ProteinsSequence AlignmentConceptsPore-forming activityProtein functionConformational changesMitochondrial membrane lipidsLipid membrane environmentSite-directed mutagenesisProfound conformational changesNovel mechanistic insightsMembrane integrationCanonical BH3Homology regionMembrane environmentMembrane permeabilizationMitochondrial lipidsMinimal domainMembrane lipidsMitochondrial levelGroove interactionsProapoptotic partnersMcl1 activityMechanistic insightsMembrane activityModel membranesFluorescence spectroscopic analysisPermeabilization
2013
Proapoptotic Bax and Bak Proteins Form Stable Protein-permeable Pores of Tunable Size
Bleicken S, Landeta O, Landajuela A, Basañez G, García-Sáez A. Proapoptotic Bax and Bak Proteins Form Stable Protein-permeable Pores of Tunable Size. Journal Of Biological Chemistry 2013, 288: 33241-33252. PMID: 24100034, PMCID: PMC3829170, DOI: 10.1074/jbc.m113.512087.Peer-Reviewed Original ResearchConceptsMitochondrial outer membraneApoptotic poreOuter membraneMitochondrial apoptotic factorsSingle vesicle levelActivity of BaxProapoptotic protein BaxProteinaceous channelsVesicle levelMembrane permeabilizationBak proteinForm poresProtein BaxProapoptotic BaxCardiolipin concentrationCytochrome cApoptotic factorsBaxBakPermeabilizationProteinSimilar mechanismMembraneMembrane poresProtein concentration