2014
Histone Variant H2A.X Deposition Pattern Serves as a Functional Epigenetic Mark for Distinguishing the Developmental Potentials of iPSCs
Wu T, Liu Y, Wen D, Tseng Z, Tahmasian M, Zhong M, Rafii S, Stadtfeld M, Hochedlinger K, Xiao A. Histone Variant H2A.X Deposition Pattern Serves as a Functional Epigenetic Mark for Distinguishing the Developmental Potentials of iPSCs. Cell Stem Cell 2014, 15: 281-294. PMID: 25192463, DOI: 10.1016/j.stem.2014.06.004.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsLineage gene expressionHistone variant H2A.XCell lineage commitmentDevelopmental potentialMouse iPSC linesIPSC linesPluripotent stem cell (iPSC) technologyEpigenetic marksLineage genesEpigenetic mechanismsLineage commitmentLineage differentiationExtraembryonic differentiationStem cell technologyGene expressionTetraploid complementationIPSC clonesIPSC qualityStem cellsFunctional markersH2A.XDifferentiationIPSCsComplementation
2009
A distinct H2A.X isoform is enriched in Xenopus laevis eggs and early embryos and is phosphorylated in the absence of a checkpoint
Shechter D, Chitta RK, Xiao A, Shabanowitz J, Hunt DF, Allis CD. A distinct H2A.X isoform is enriched in Xenopus laevis eggs and early embryos and is phosphorylated in the absence of a checkpoint. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 749-754. PMID: 19131518, PMCID: PMC2630098, DOI: 10.1073/pnas.0812207106.Peer-Reviewed Original ResearchConceptsDNA damageEarly embryosSignificance of phosphorylationTerminal consensus sequenceExogenous DNA damageDNA damage responseCell-free egg extractsPhospho-specific antibodiesEarly cell cyclesFrog Xenopus laevisMulticellular organismsH2A variantsXenopus tropicalisDamage responseUnannotated isoformsMammalian cellsHistone H2A.XSomatic cellsCheckpoint conditionsXenopus laevis eggsAquatic speciesEgg extractsConsensus sequenceCell cycleH2A.X