2024
Understanding the significance of oxygen tension on the biology of Plasmodium falciparum blood stages: From the human body to the laboratory
Nahid D, Coffey K, Bei A, Cordy R. Understanding the significance of oxygen tension on the biology of Plasmodium falciparum blood stages: From the human body to the laboratory. PLOS Pathogens 2024, 20: e1012514. PMID: 39298535, PMCID: PMC11412506, DOI: 10.1371/journal.ppat.1012514.Peer-Reviewed Original ResearchConceptsRed blood cellsIntraerythrocytic developmentReactive oxygen speciesPlasmodium falciparum blood stagesMultiple organ systemsP. falciparum mitochondrionStatus of hemoglobinBlood stagesPlasmodium falciparumReactive oxygen species productionO2-sensing mechanismIn vitro experimentsPlasmodiumBlood cellsOxygenation statusOrgan systemsFunctional changesParasite growthOxidative stressOxygen tensionMosquito hostCulture systemDeep tissuesOxygen species
2017
Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human Erythrocytes
Dankwa S, Chaand M, Kanjee U, Jiang RHY, Nobre LV, Goldberg JM, Bei AK, Moechtar MA, Grüring C, Ahouidi AD, Ndiaye D, Dieye TN, Mboup S, Weekes MP, Duraisingh MT. Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human Erythrocytes. Infection And Immunity 2017, 85: 10.1128/iai.00074-17. PMID: 28760933, PMCID: PMC5607420, DOI: 10.1128/iai.00074-17.Peer-Reviewed Original ResearchConceptsInvasion pathwaysGlycophorin CGlycophorin BExpression levelsShort hairpin RNA transductionLigand-receptor interactionsErythroid progenitor cellsKnockout linesGenetic evidenceBioinformatics analysisKnockdown cellsProteomic profilingTranscript levelsMultiple proteinsHuman erythrocytesExtensive variationParasite invasionProgenitor cellsInvasion ligandsB expression levelsLaboratory strainsGPC receptorsCulture-adapted strainsPathwayReceptor usageFunctional Analysis Reveals Geographical Variation in Inhibitory Immune Responses Against a Polymorphic Malaria Antigen
Bei AK, Ahouidi AD, Dvorin JD, Miura K, Diouf A, Ndiaye D, Premji Z, Diakite M, Mboup S, Long CA, Duraisingh MT. Functional Analysis Reveals Geographical Variation in Inhibitory Immune Responses Against a Polymorphic Malaria Antigen. The Journal Of Infectious Diseases 2017, 216: 267-275. PMID: 28605544, PMCID: PMC5853457, DOI: 10.1093/infdis/jix280.Peer-Reviewed Original ResearchConceptsInhibitory immune responsesVaccine candidate antigenImmune responseTransgenic parasite linesMalaria-endemic regionsReticulocyte-binding protein homologuesMalaria vaccine candidateParasite linesWild-type controlsTotal IgGHumoral responseMalaria antigensAntibody responseVaccine candidatesCandidate antigensMalaria endemicityGrowth inhibition assaysInvasion ligandsAntigenic specificityImmunogenic domainsSpecific antibodiesEndemic sitesAntigenStandardized toolsInhibition assays
2016
Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite
Dankwa S, Lim C, Bei AK, Jiang RH, Abshire JR, Patel SD, Goldberg JM, Moreno Y, Kono M, Niles JC, Duraisingh MT. Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite. Nature Communications 2016, 7: 11187. PMID: 27041489, PMCID: PMC4822025, DOI: 10.1038/ncomms11187.Peer-Reviewed Original ResearchConceptsInvasion ligandsSialic acid-independent pathwaysHuman RBCsZoonotic malaria parasiteAbsence of Neu5GcPlasmodium knowlesiMalaria parasitesCell surface receptorsN-glycolylneuraminic acidZoonotic parasitesSialic acid variantsSurface receptorsRBCsReceptorsNeu5GcMacaquesInvasionParasitesCMAHAcetylneuraminic acidAcid variantsHumansMalariaKnowlesi
2015
Malaria Vaccine Development: Focusing Field Erythrocyte Invasion Studies on Phenotypic Diversity The West African Merozoite Invasion Network (WAMIN)
: T, Ahouidi AD, Amambua-Ngwa A, Awandare GA, Bei AK, Conway DJ, Diakite M, Duraisingh MT, Rayner JC, Zenonos ZA. Malaria Vaccine Development: Focusing Field Erythrocyte Invasion Studies on Phenotypic Diversity The West African Merozoite Invasion Network (WAMIN). Trends In Parasitology 2015, 32: 274-283. PMID: 26725306, PMCID: PMC7021314, DOI: 10.1016/j.pt.2015.11.009.Peer-Reviewed Original ResearchConceptsVaccine candidatesBlood-stage vaccine candidatePromising vaccine targetPlasmodium falciparum merozoitesRelevant evidence baseFalciparum merozoitesVaccine targetsEvidence baseErythrocyte invasionStandardized assaysParasite survivalInvasion phenotypeInvasion studiesTrue extentField isolatesInvasionMost studiesLigands differsThe Clinically Tested Gardos Channel Inhibitor Senicapoc Exhibits Antimalarial Activity
Tubman VN, Mejia P, Shmukler BE, Bei AK, Alper SL, Mitchell JR, Brugnara C, Duraisingh MT. The Clinically Tested Gardos Channel Inhibitor Senicapoc Exhibits Antimalarial Activity. Antimicrobial Agents And Chemotherapy 2015, 60: 613-616. PMID: 26459896, PMCID: PMC4704178, DOI: 10.1128/aac.01668-15.Peer-Reviewed Original ResearchConceptsGardos channel inhibitorChannel inhibitorsSickle cell diseaseC57BL/6 miceClinical trialsCell diseaseAntimalarial developmentPlasmodium growthErythrocyte dehydrationGardos channelSenicapocBlood stagesBiochemical profileAntimalarial activityPrimate plasmodiaVitro growthInhibitorsPatientsParasitemiaBlockadeDiseaseMiceTrialsMeasuring Plasmodium falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry
Bei AK, Duraisingh MT. Measuring Plasmodium falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry. Methods In Molecular Biology 2015, 1325: 167-186. PMID: 26450388, DOI: 10.1007/978-1-4939-2815-6_14.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ProtozoanErythrocytesFlow CytometryHumansMalaria, FalciparumPlasmodium falciparumSchizontsConceptsPlasmodium falciparum merozoite invasionParasite multiplication rateErythrocyte invasion phenotypesInvasion of erythrocytesFlow cytometry methodVaccine mechanismDisease severityInvasion inhibitionEx vivoFlow cytometryMerozoite invasionCytometry methodRing stageInvasion phenotypeInvasionResearch settingsVivoImportant determinantLow levelsAlternative pathwayParasitesParasitemia
2014
STEVOR Is a Plasmodium falciparum Erythrocyte Binding Protein that Mediates Merozoite Invasion and Rosetting
Niang M, Bei AK, Madnani KG, Pelly S, Dankwa S, Kanjee U, Gunalan K, Amaladoss A, Yeo KP, Bob NS, Malleret B, Duraisingh MT, Preiser PR. STEVOR Is a Plasmodium falciparum Erythrocyte Binding Protein that Mediates Merozoite Invasion and Rosetting. Cell Host & Microbe 2014, 16: 81-93. PMID: 25011110, PMCID: PMC4382205, DOI: 10.1016/j.chom.2014.06.004.Peer-Reviewed Original ResearchConceptsMerozoite invasionFalciparum erythrocyte membrane protein 1P. falciparum erythrocyte membrane protein 1Erythrocyte membrane protein 1Variant surface antigensErythrocyte binding proteinInfected erythrocyte surfaceErythrocyte-binding proteinsMembrane protein 1STEVOR expressionMalaria pathogenesisRed blood cell surfaceImmune evasionSurface antigenUninfected RBCsBlood cell surfaceGlycophorin CInfected RBCsErythrocyte surfaceParasite infectionProtein 1Invasive merozoitesForm rosettesSTEVORSchizont stageAn Adjustable Gas-Mixing Device to Increase Feasibility of In Vitro Culture of Plasmodium falciparum Parasites in the Field
Bei AK, Patel SD, Volkman SK, Ahouidi AD, Ndiaye D, Mboup S, Wirth DF. An Adjustable Gas-Mixing Device to Increase Feasibility of In Vitro Culture of Plasmodium falciparum Parasites in the Field. PLOS ONE 2014, 9: e90928. PMID: 24603696, PMCID: PMC3946284, DOI: 10.1371/journal.pone.0090928.Peer-Reviewed Original ResearchMeSH KeywordsAutomation, LaboratoryCarbon DioxideErythrocytesHumansNitrogenOxygenPlasmodium falciparumPrimary Cell Culture
2013
Inhibitory Humoral Responses to the Plasmodium falciparum Vaccine Candidate EBA-175 Are Independent of the Erythrocyte Invasion Pathway
Badiane AS, Bei AK, Ahouidi AD, Patel SD, Salinas N, Ndiaye D, Sarr O, Ndir O, Tolia NH, Mboup S, Duraisingh MT. Inhibitory Humoral Responses to the Plasmodium falciparum Vaccine Candidate EBA-175 Are Independent of the Erythrocyte Invasion Pathway. MSphere 2013, 20: 1238-1245. PMID: 23761656, PMCID: PMC3754519, DOI: 10.1128/cvi.00135-13.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayEBA-175Human antibodiesAffinity-purified human antibodiesBlood-stage vaccine candidateParasite strainsSialic acid-dependent mannerParasite invasionPresence of antibodiesErythrocyte-binding domainMultiple ligand-receptor interactionsEx vivo assaysPooled patient plasmaSenegalese parasitesAlternative invasion pathwaysHumoral responseVaccine candidatesPatient plasmaSenegalese patientsLigand-receptor interactionsClinical isolatesInvasion inhibitionVaccine designEx vivoFunctional inhibitionExpansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans
Lim C, Hansen E, DeSimone TM, Moreno Y, Junker K, Bei A, Brugnara C, Buckee CO, Duraisingh MT. Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans. Nature Communications 2013, 4: 1638. PMID: 23535659, PMCID: PMC3762474, DOI: 10.1038/ncomms2612.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsErythrocytesHumansMacaca mulattaPlasmodium knowlesiZoonosesConceptsRed blood cellsParasite densityP. knowlesiHuman bloodPlasmodium falciparum infectionMalaria parasite Plasmodium knowlesiYoung red blood cellsZoonotic malaria parasiteHigh parasite densityOld red blood cellsFalciparum infectionClinical infectionSevere diseaseWide age rangeNormal human bloodPlasmodium knowlesiMalaria parasitesBlood cellsImportant zoonosisAge rangeInfectionBloodKnowlesiYoung cellsCulture system
2012
Functional analysis of erythrocyte determinants of Plasmodium infection
Bei AK, Duraisingh MT. Functional analysis of erythrocyte determinants of Plasmodium infection. International Journal For Parasitology 2012, 42: 575-582. PMID: 22726752, PMCID: PMC3383627, DOI: 10.1016/j.ijpara.2012.03.006.Peer-Reviewed Original ResearchMeSH KeywordsEndocytosisErythrocytesHost-Pathogen InteractionsHumansMalariaModels, BiologicalPlasmodium falciparumConceptsFunctional analysisErythrocyte determinantsObligate intracellular pathogensPlasmodium infectionTransgenic approachesHuman erythrocytes resultsNatural variationErythrocyte invasionIntracellular pathogensPlasmodium falciparum parasitesUnexplored frontierClinical manifestationsMalaria researchErythrocytes resultsFalciparum parasitesInvasionMalarial diseaseChemical biologicalInfectionErythrocytesParasitesPathogensDeterminantsRemodellingDiscovery
2011
Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum
Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner JC, Wright GJ. Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Nature 2011, 480: 534-537. PMID: 22080952, PMCID: PMC3245779, DOI: 10.1038/nature10606.Peer-Reviewed Original ResearchConceptsPlasmodium falciparum merozoitesInvasion of erythrocytesMalaria infectionFalciparum merozoitesAntimalarial therapeuticsP. falciparumBlood stagesPlasmodium falciparumErythrocyte invasionParasite invasionInvasionFalciparumReceptor essentialField strainsBasiginErythrocytesPfRH5VaccineInfectionAntibodies
2010
In Vitro Genetic Analysis of an Erythrocyte Determinant of Malaria Infection
Bei AK, Brugnara C, Duraisingh MT. In Vitro Genetic Analysis of an Erythrocyte Determinant of Malaria Infection. The Journal Of Infectious Diseases 2010, 202: 1722-1727. PMID: 20958212, PMCID: PMC3107553, DOI: 10.1086/657157.Peer-Reviewed Original ResearchConceptsGenetic analysisErythrocyte determinantsSystematic functional analysisDirect genetic analysisErythrocyte biologyInvasion of erythrocytesIsogenic backgroundP. falciparum invasionGene expressionFunctional analysisParasite invasionObligatory stepLentiviral transductionHost receptorsGlycophorin ALife cyclePlasmodium falciparumCulture systemInvasionMalaria infectionUnambiguous identificationTransductionBiologyParasitesErythrocytesA flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum
Bei AK, DeSimone TM, Badiane AS, Ahouidi AD, Dieye T, Ndiaye D, Sarr O, Ndir O, Mboup S, Duraisingh MT. A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum. American Journal Of Hematology 2010, 85: 234-237. PMID: 20196166, PMCID: PMC3089760, DOI: 10.1002/ajh.21642.Peer-Reviewed Original ResearchConceptsParasite invasionMalaria parasite Plasmodium falciparumParasite Plasmodium falciparumPlasmodium falciparumFlow cytometry-based assayParasite multiplication rateFlow cytometry-based methodParasite developmentCytometry-based assayCytometry-based methodRed blood cellsDisease severityDye SYBR Green IRing stageVariable invasionMultiplication rateBlood cellsInvasionSYBR Green IHuman erythrocytesFalciparumGreen IImportant determinantErythrocytesParasitemiaFunctional diversification between two related Plasmodium falciparum merozoite invasion ligands is determined by changes in the cytoplasmic domain
Dvorin JD, Bei AK, Coleman BI, Duraisingh MT. Functional diversification between two related Plasmodium falciparum merozoite invasion ligands is determined by changes in the cytoplasmic domain. Molecular Microbiology 2010, 75: 990-1006. PMID: 20487292, PMCID: PMC3627358, DOI: 10.1111/j.1365-2958.2009.07040.x.Peer-Reviewed Original ResearchConceptsCytoplasmic domainFunctional diversificationInvasion pathwaysMulti-gene familyDifferent erythrocyte receptorsAmino acid regionAlternative invasion pathwaysN-terminal amino acidsGenomic regionsProtein homologueTransmembrane domainTransmembrane regionPfRh proteinsErythrocyte receptorEfficient invasionAcid regionInvasive merozoitesHost erythrocytePfRh2bAmino acidsPfRh2aPathway utilizationApical surfaceInvasion ligandsEctodomain
2009
Erythrocyte invasion profiles are associated with a common invasion ligand polymorphism in Senegalese isolates of Plasmodium falciparum
LANTOS PM, AHOUIDI AD, BEI AK, JENNINGS CV, SARR O, NDIR O, WIRTH DF, MBOUP S, DURAISINGH MT. Erythrocyte invasion profiles are associated with a common invasion ligand polymorphism in Senegalese isolates of Plasmodium falciparum. Parasitology 2009, 136: 1-9. PMID: 19126266, DOI: 10.1017/s0031182008005167.Peer-Reviewed Original ResearchConceptsErythrocyte receptorSialic acid-independent invasionBlood group O patientsDifferent erythrocyte receptorsGroup O patientsSenegalese isolatesParasite multiplication ratePlasmodium falciparum parasitesMultiple ligand-receptor interactionsO patientsFalciparum parasitesLarge sequence deletionsVariant expression levelParasite isolatesPlasmodium falciparumErythrocyte invasionSelectivity indexReceptorsExpression levelsMajor polymorphismsInvasionInvasion pathwaysPossible determinantsParasitesHuman erythrocytes
2007
Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal
Jennings CV, Ahouidi AD, Zilversmit M, Bei AK, Rayner J, Sarr O, Ndir O, Wirth DF, Mboup S, Duraisingh MT. Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal. Infection And Immunity 2007, 75: 3531-3538. PMID: 17470537, PMCID: PMC1932937, DOI: 10.1128/iai.00122-07.Peer-Reviewed Original ResearchConceptsInvasion pathwaysSequence polymorphismsMolecular analysisDistinct invasion pathwaysC-terminal regionMalaria parasitesSpecific sequence variantsGene amplificationHuman malaria parasiteLigand-receptor interactionsExpression polymorphismsRepeat regionProtein expression levelsSequence deletionsHomolog familyMultiple ligand-receptor interactionsLarge sequence deletionsSequence variantsSignificant sequence polymorphismErythrocyte invasionErythrocyte receptorP. falciparum parasitesExpression levelsPfRh1Low transmission areasVariant merozoite protein expression is associated with erythrocyte invasion phenotypes in Plasmodium falciparum isolates from Tanzania
Bei AK, Membi CD, Rayner JC, Mubi M, Ngasala B, Sultan AA, Premji Z, Duraisingh MT. Variant merozoite protein expression is associated with erythrocyte invasion phenotypes in Plasmodium falciparum isolates from Tanzania. Molecular And Biochemical Parasitology 2007, 153: 66-71. PMID: 17303262, DOI: 10.1016/j.molbiopara.2007.01.007.Peer-Reviewed Original Research