2015
Measuring Plasmodium falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry
Bei AK, Duraisingh MT. Measuring Plasmodium falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry. Methods In Molecular Biology 2015, 1325: 167-186. PMID: 26450388, DOI: 10.1007/978-1-4939-2815-6_14.Peer-Reviewed Original ResearchConceptsPlasmodium falciparum merozoite invasionParasite multiplication rateErythrocyte invasion phenotypesInvasion of erythrocytesFlow cytometry methodVaccine mechanismDisease severityInvasion inhibitionEx vivoFlow cytometryMerozoite invasionCytometry methodRing stageInvasion phenotypeInvasionResearch settingsVivoImportant determinantLow levelsAlternative pathwayParasitesParasitemia
2010
A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum
Bei AK, DeSimone TM, Badiane AS, Ahouidi AD, Dieye T, Ndiaye D, Sarr O, Ndir O, Mboup S, Duraisingh MT. A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum. American Journal Of Hematology 2010, 85: 234-237. PMID: 20196166, PMCID: PMC3089760, DOI: 10.1002/ajh.21642.Peer-Reviewed Original ResearchConceptsParasite invasionMalaria parasite Plasmodium falciparumParasite Plasmodium falciparumPlasmodium falciparumFlow cytometry-based assayParasite multiplication rateFlow cytometry-based methodParasite developmentCytometry-based assayCytometry-based methodRed blood cellsDisease severityDye SYBR Green IRing stageVariable invasionMultiplication rateBlood cellsInvasionSYBR Green IHuman erythrocytesFalciparumGreen IImportant determinantErythrocytesParasitemia
2009
Erythrocyte invasion profiles are associated with a common invasion ligand polymorphism in Senegalese isolates of Plasmodium falciparum
LANTOS PM, AHOUIDI AD, BEI AK, JENNINGS CV, SARR O, NDIR O, WIRTH DF, MBOUP S, DURAISINGH MT. Erythrocyte invasion profiles are associated with a common invasion ligand polymorphism in Senegalese isolates of Plasmodium falciparum. Parasitology 2009, 136: 1-9. PMID: 19126266, DOI: 10.1017/s0031182008005167.Peer-Reviewed Original ResearchConceptsErythrocyte receptorSialic acid-independent invasionBlood group O patientsDifferent erythrocyte receptorsGroup O patientsSenegalese isolatesParasite multiplication ratePlasmodium falciparum parasitesMultiple ligand-receptor interactionsO patientsFalciparum parasitesLarge sequence deletionsVariant expression levelParasite isolatesPlasmodium falciparumErythrocyte invasionSelectivity indexReceptorsExpression levelsMajor polymorphismsInvasionInvasion pathwaysPossible determinantsParasitesHuman erythrocytes