1998
Immunochemical characterization of Na+/H+exchanger isoform NHE4
Pizzonia J, Biemesderfer D, Abu-Alfa A, Wu M, Exner M, Isenring P, Igarashi P, Aronson P. Immunochemical characterization of Na+/H+exchanger isoform NHE4. American Journal Of Physiology 1998, 275: f510-f517. PMID: 9755122, DOI: 10.1152/ajprenal.1998.275.4.f510.Peer-Reviewed Original ResearchConceptsSubcellular localizationFusion proteinWestern blot analysisBlot analysisBroad tissue distributionBasolateral membraneTransport proteinsMembrane fractionProteinEpithelial cellsSkeletal muscleGradient centrifugationTissue distributionPercoll gradient centrifugationCellsGastric glandsNHE4MembraneImmunochemical characterizationMammalianLocalizationRat tissuesImmunochemical techniquesMonoclonal antibodiesPolypeptide
1997
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney
Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. American Journal Of Physiology 1997, 273: f289-f299. PMID: 9277590, DOI: 10.1152/ajprenal.1997.273.2.f289.Peer-Reviewed Original ResearchConceptsProximal tubulesRat kidneyMonoclonal antibodiesLoop of HenleHigh-resolution immunocytochemical studiesProximal tubule cellsPrevious immunochemical studiesIsoform-specific monoclonal antibodiesNeonatal rat kidneyTubule cellsImmunocytochemical studyThick limbImmunocytochemical techniquesKidneyLAP cellsRenal epitheliumNHE3Immunochemical studiesHigh-resolution immunocytochemical techniqueAdultsLater stagesNephron developmentExpression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Experimental Nephrology 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1AntibodiesSodium-hydrogen exchange isoform expression in blood cells: implications for studies in diabetes mellitus
Rutherford P, Pizzonia J, Abu-Alfa A, Biemesderfer D, Reilly R, Aronson P. Sodium-hydrogen exchange isoform expression in blood cells: implications for studies in diabetes mellitus. Experimental And Clinical Endocrinology & Diabetes 1997, 105: 13-16. PMID: 9288535, DOI: 10.1055/s-0029-1211787.Peer-Reviewed Original ResearchConceptsPeripheral blood cellsPeripheral blood cell preparationsDiabetes mellitusBlood cell preparationsBlood cellsNHE activityPathophysiological roleNa-H exchange activityNHE-1Vascular smooth muscle cell proliferationNHE-3Smooth muscle cell proliferationProgressive renal impairmentNHE isoformsVascular smooth muscleDirect pathophysiological roleCell preparationsMuscle cell proliferationPlatelet membranesNa-H exchangeRenal proximal tubulesNHE isoforms 1Sodium-hydrogen exchangeRenal impairmentNondiabetic controls
1992
Uremic Pancreopathy: Impaired Secretory Function In Vitro
Abu-Alfa A, Mujais S. Uremic Pancreopathy: Impaired Secretory Function In Vitro. American Journal Of Kidney Diseases 1992, 20: 585-588. PMID: 1462987, DOI: 10.1016/s0272-6386(12)70223-8.Peer-Reviewed Original ResearchConceptsSecretory responsePair-fed shamResult of derangementsExocrine pancreatic functionLeucine incorporationRenal failurePancreatic functionProtein synthesisProtein secretionSecretory functionPancreatic aciniExocrine pancreasAbnormalitiesCarbacholSecretionPresent studyAciniDNA contentUremiaDerangementShamPancreasRats
1989
Gentamicin nephrotoxicity in extrahepatic cholestasis: Modulation by dietary calcium
Vakil N, Abu‐Alfa A, Mujais S. Gentamicin nephrotoxicity in extrahepatic cholestasis: Modulation by dietary calcium. Hepatology 1989, 9: 519-524. PMID: 2925154, DOI: 10.1002/hep.1840090402.Peer-Reviewed Original ResearchConceptsDietary calcium supplementationGentamicin nephrotoxicityCalcium supplementationLigation animalsExtrahepatic cholestasisCommon bile duct ligation groupCommon bile duct ligation ratsBile duct ligation groupCommon bile duct obstructionBile duct ligation ratsMale Sprague-Dawley ratsUrinary calcium excretionHigh calcium dietBile duct obstructionBile duct ligationSprague-Dawley ratsCourse of therapyExperimental extrahepatic cholestasisSevere azotemiaCalcium excretionConcurrent administrationDuct obstructionGentamicin administrationSham ratsDietary calcium