Granulosa cell and oocyte mitochondrial abnormalities in a mouse model of fragile X primary ovarian insufficiency
Dioguardi C, Uslu B, Haynes M, Kurus M, Gul M, Miao DQ, De Santis L, Ferrari M, Bellone S, Santin A, Giulivi C, Hoffman G, Usdin K, Johnson J. Granulosa cell and oocyte mitochondrial abnormalities in a mouse model of fragile X primary ovarian insufficiency. Molecular Human Reproduction 2016, 22: 384-396. PMID: 26965313, PMCID: PMC4884918, DOI: 10.1093/molehr/gaw023.Peer-Reviewed Original ResearchConceptsMitochondrial DNA copy numberDNA copy numberFMR1 proteinMitochondrial contentTranslation productsRepeat associated non-ATG translationFMR1 mRNACGG repeatsCopy numberMitochondrial dysfunctionMitochondrial gene expressionNon-ATG translationCGG-repeat tractQuantitative RT-PCR analysisOptic atrophy 1Mitochondrial structural abnormalitiesPrimary ovarian insufficiencyGranulosa cellsMutant genotypesGene dosage effectMetaphase II eggsMitochondrial genesMitochondrial architectureMitochondrial fusionRT-PCR analysis