2024
Heterogeneous gene expression during early arteriovenous fistula remodeling suggests that downregulation of metabolism predicts adaptive venous remodeling
Ohashi Y, Protack C, Aoyagi Y, Gonzalez L, Thaxton C, Zhang W, Kano M, Bai H, Yatsula B, Alves R, Hoshina K, Schneider E, Long X, Perry R, Dardik A. Heterogeneous gene expression during early arteriovenous fistula remodeling suggests that downregulation of metabolism predicts adaptive venous remodeling. Scientific Reports 2024, 14: 13287. PMID: 38858395, PMCID: PMC11164895, DOI: 10.1038/s41598-024-64075-8.Peer-Reviewed Original ResearchConceptsGene expression patternsArteriovenous fistula groupArteriovenous fistulaExpression patternsHeterogeneous gene expressionGene expression changesOutcomes of arteriovenous fistulaVenous remodelingArteriovenous fistula maturationPostoperative day 7Reduce patient morbidityRNA sequencingUpregulation of metabolismBioinformatics analysisGene expressionDownregulation of metabolismMetabolic pathwaysExpression changesGenesAVF maturationAVF remodelingFistula creationC57BL/6 miceClinical outcomesPatient morbidityAbstract 3092: Smooth Muscle Cell Tgf-β Receptor Knockout Improves Arteriovenous Fistula Maturation Via Attenuation Of Autophagy
Zhang W, Bai H, Gonzalez L, Li Z, Ohashi Y, Thaxton C, aoyagi Y, Yatsula B, Kano M, Xie Y, Cai Y, Chang S, Dardik A. Abstract 3092: Smooth Muscle Cell Tgf-β Receptor Knockout Improves Arteriovenous Fistula Maturation Via Attenuation Of Autophagy. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: a3092-a3092. DOI: 10.1161/atvb.44.suppl_1.3092.Peer-Reviewed Original ResearchChronic kidney diseaseSmooth muscle cellsEnd-stage renal diseaseArteriovenous fistulaHuman arteriovenous fistulaeControl miceC57BL/6J miceCKD showRate of primary failureBasilic vein transpositionTGF-bModel of arteriovenous fistulaTGF-b1 expressionArteriovenous fistula failureArteriovenous fistula maturationWild type miceControl C57BL/6J miceIncreased markers of autophagyTGF-b signalingVein transpositionAortocaval fistulaAVF maturationReceptor knockoutMT/mG miceSham nephrectomy
2023
Arterial Diameter and Percentage of Monocytes are Sex-Dependent Predictors of Early Arteriovenous Fistula Maturation
Satam K, Setia O, Moore M, Schneider E, Chaar C, Dardik A. Arterial Diameter and Percentage of Monocytes are Sex-Dependent Predictors of Early Arteriovenous Fistula Maturation. Annals Of Vascular Surgery 2023, 93: 128-136. PMID: 36812979, PMCID: PMC10277224, DOI: 10.1016/j.avsg.2023.01.052.Peer-Reviewed Original ResearchConceptsFistula maturationArteriovenous fistula maturationArterial diameterArteriovenous fistulaFistula creationBrachial artery diameterPrimary arteriovenous fistulaPercentage of neutrophilsPeak systolic velocityPercentage of monocytesBrachial-cephalic fistulaElectronic medical recordsSex differencesFistula utilizationInferior patencyPostoperative ultrasoundUnassisted maturationYears postprocedureSingle centerArtery diameterArterial inflowArterial velocitySystolic velocityMedical recordsMonocyte percentage
2021
Inhibition of T-Cells by Cyclosporine A Reduces Macrophage Accumulation to Regulate Venous Adaptive Remodeling and Increase Arteriovenous Fistula Maturation
Matsubara Y, Kiwan G, Liu J, Gonzalez L, Langford J, Gao M, Gao X, Taniguchi R, Yatsula B, Furuyama T, Matsumoto T, Komori K, Dardik A. Inhibition of T-Cells by Cyclosporine A Reduces Macrophage Accumulation to Regulate Venous Adaptive Remodeling and Increase Arteriovenous Fistula Maturation. Arteriosclerosis Thrombosis And Vascular Biology 2021, 41: e160-e174. PMID: 33472405, PMCID: PMC7904667, DOI: 10.1161/atvbaha.120.315875.Peer-Reviewed Original ResearchConceptsVascular wall thickeningT helper type 1 (Th1) cellsSuccessful AVF maturationAVF maturationRegulatory T cellsMacrophage accumulationT cellsType 1 cellsArteriovenous fistulaVenous remodelingWall thickeningMouse aortocaval fistula modelT helper type 2T helper type 2 cellsAdaptive remodelingM2 macrophage accumulationPrimary success rateAortocaval fistula modelArteriovenous fistula maturationPreferred vascular accessAnti-inflammatory macrophagesT cell functionWild-type miceEffect of CsAType 2 cellsChapter 13 Assessment of female sex in preclinical vascular models
Gao M, Gorecka J, Liu J, Dardik A. Chapter 13 Assessment of female sex in preclinical vascular models. 2021, 349-385. DOI: 10.1016/b978-0-12-822959-0.00005-5.ChaptersAneurysm formationSequelae of atherosclerosisArteriovenous fistula maturationPathophysiology of atherosclerosisCause of deathPositive vessel remodelingAtherosclerotic complicationsFistula maturationFemale sexVenous remodelingClinical trialsInflammatory responseSex hormonesCardiovascular diseaseDisease outcomePreclinical studiesAnimal modelsProtective roleAtherosclerosisLipid metabolismVessel remodelingFemale animalsOxidative stressNational InstituteNumber of participants
2020
TGFβ (Transforming Growth Factor-Beta)–Activated Kinase 1 Regulates Arteriovenous Fistula Maturation
Hu H, Lee SR, Bai H, Guo J, Hashimoto T, Isaji T, Guo X, Wang T, Wolf K, Liu S, Ono S, Yatsula B, Dardik A. TGFβ (Transforming Growth Factor-Beta)–Activated Kinase 1 Regulates Arteriovenous Fistula Maturation. Arteriosclerosis Thrombosis And Vascular Biology 2020, 40: e203-e213. PMID: 32460580, PMCID: PMC7316601, DOI: 10.1161/atvbaha.119.313848.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaArteriovenous Shunt, SurgicalCells, CulturedCollagen Type IEndothelial CellsFibronectinsHumansJNK Mitogen-Activated Protein KinasesMaleMAP Kinase Kinase KinasesMechanotransduction, CellularMice, Inbred C57BLP38 Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalVascular PatencyVascular RemodelingVena Cava, InferiorConceptsArteriovenous fistulaAVF maturationNoncanonical TGFβSuccessful AVF maturationArteriovenous fistula maturationNovel therapeutic approachesTAK1 functionsFistula maturationHemodialysis accessDeposition of ECMLuminal diameterMouse endothelial cellsMaturation failureTherapeutic approachesLumen dilationControl veinsWall thickeningAVF modelOptimal conduitArterial magnitudesEndothelial cellsTAK1 phosphorylationCollagen 1Growth factorLaminar shear stress