Featured Publications
High-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation
Oeck S, Malewicz NM, Krysztofiak A, Turchick A, Jendrossek V, Glazer PM. High-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation. Scientific Reports 2019, 9: 3148. PMID: 30816253, PMCID: PMC6395640, DOI: 10.1038/s41598-019-39760-8.Peer-Reviewed Original ResearchConceptsDNA damage sitesDNA repair proteinsProtein of interestHistone dynamicsHistone phosphorylationProtein fociHigh-throughput analysisProtein modificationRepair proteinsDNA damageDamage sitesSingle nucleusMultiple fluorescence channelsHigh-throughput evaluationRepair kineticsProtein migrationProteinAnalysis of accumulationFluorescence channelsPhosphorylationDNACellsAccumulationLocalizationMigration
2022
The Pathogenic R3052W BRCA2 Variant Disrupts Homology-Directed Repair by Failing to Localize to the Nucleus
Jimenez-Sainz J, Krysztofiak A, Garbarino J, Rogers F, Jensen RB. The Pathogenic R3052W BRCA2 Variant Disrupts Homology-Directed Repair by Failing to Localize to the Nucleus. Frontiers In Genetics 2022, 13: 884210. PMID: 35711920, PMCID: PMC9197106, DOI: 10.3389/fgene.2022.884210.Peer-Reviewed Original ResearchDominant-negative alleleDNA damage responseDNA repair functionDNA strand exchangeHomology-directed repairGenome instabilityCellular functionsDamage responseDNA bindingNegative allelesStrand exchangeRepair functionGermline missense variantsCell survivalFunction mutationsMissense variantsRAD51Pathogenic allelesSimple lossPARP inhibitorsCytoplasmProteinAllelesHomologyDNA