2020
Chemical mutagenesis of a GPCR ligand: Detoxifying “inflammo-attraction” to direct therapeutic stem cell migration
Lee J, Zhang R, Yan M, Duggineni S, Wakeman D, Niles W, Feng Y, Chen J, Hamblin M, Han E, Gonzalez R, Fang X, Zhu Y, Wang J, Xu Y, Wenger D, Seyfried T, An J, Sidman R, Huang Z, Snyder E. Chemical mutagenesis of a GPCR ligand: Detoxifying “inflammo-attraction” to direct therapeutic stem cell migration. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 31177-31188. PMID: 33219123, PMCID: PMC7733796, DOI: 10.1073/pnas.1911444117.Peer-Reviewed Original ResearchConceptsNeural stem cellsCXCR4 agonistPrototypical neurodegenerative diseaseDonor-derived cellsStem cellsCerebral cortexCNS injuryInflammatory chemokinesHost inflammationUndesirable inflammationCXCL-12Mouse modelTherapeutic impactChemokine CXCL12Stem cell propertiesCell engagementNeurodegenerative diseasesStem cell migrationNSC migrationAgonistsSynthetic functionInflammationChemokinesFundamental stem cell propertiesCXCL12
2014
Homing of Neural Stem Cells From the Venous Compartment Into a Brain Infarct Does Not Involve Conventional Interactions With Vascular Endothelium
Goncharova V, Das S, Niles W, Schraufstatter I, Wong A, Povaly T, Wakeman D, Miller L, Snyder E, Khaldoyanidi S. Homing of Neural Stem Cells From the Venous Compartment Into a Brain Infarct Does Not Involve Conventional Interactions With Vascular Endothelium. Stem Cells Translational Medicine 2014, 3: 229-240. PMID: 24396034, PMCID: PMC3925049, DOI: 10.5966/sctm.2013-0052.Peer-Reviewed Original ResearchConceptsHuman neural stem cellsNeural stem cellsIntravenous administrationStromal cell-derived factor-1αEx vivo fucosylationCutaneous lymphocyte antigenBlood-brain barrierStem cellsMortality of ratsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsImplantation of cellsBrain infarctsVein endothelial cellsLymphocyte antigenVascular endotheliumIntracranial injectionVenous compartmentCell surface moietiesFactor-1αIntravascular spaceEndothelial cellsAdministrationLuminal surfaceRemoval of fucose
2007
Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells
Redmond DE, Bjugstad KB, Teng YD, Ourednik V, Ourednik J, Wakeman DR, Parsons XH, Gonzalez R, Blanchard BC, Kim SU, Gu Z, Lipton SA, Markakis EA, Roth RH, Elsworth JD, Sladek JR, Sidman RL, Snyder EY. Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 12175-12180. PMID: 17586681, PMCID: PMC1896134, DOI: 10.1073/pnas.0704091104.Peer-Reviewed Original ResearchConceptsHuman neural stem cellsSubstantia nigraParkinson's diseaseNeural stem cellsTyrosine hydroxylaseBehavioral improvementModel of PDHost substantia nigraStem cellsResponsive progenitor cellsAlpha-synuclein aggregationDA markersNigrostriatal circuitryParkinsonian primatesParkinsonian signsNeuronal numberDA levelsFunctional improvementParkinson modelImmunopositive cellsNormalizing effectDA phenotypeHomeostatic effectsNumber of diseasesProgenitor cells
2006
Adhesive Interactions Between Human Neural Stem Cells and Inflamed Human Vascular Endothelium Are Mediated by Integrins
Mueller F, Serobyan N, Schraufstatter I, DiScipio R, Wakeman D, Loring J, Snyder E, Khaldoyanidi S. Adhesive Interactions Between Human Neural Stem Cells and Inflamed Human Vascular Endothelium Are Mediated by Integrins. Stem Cells 2006, 24: 2367-2372. PMID: 17071855, PMCID: PMC2885956, DOI: 10.1634/stemcells.2005-0568.Peer-Reviewed Original ResearchMeSH KeywordsCell AdhesionCell LineCell MovementEmbryonic Stem CellsEndothelial CellsHumansImmunohistochemistryInflammationIntegrin alpha2Integrin alpha6Integrin beta1IntegrinsNeuronsReverse Transcriptase Polymerase Chain ReactionStress, MechanicalTumor Necrosis Factor-alphaUp-RegulationVascular Cell Adhesion Molecule-1