2020
Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo
Hiller B, Marmion D, Gross R, Thompson C, Chavez C, Brundin P, Wakeman D, McMahon C, Kordower J. Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo. Stem Cells Translational Medicine 2020, 10: 278-290. PMID: 32997443, PMCID: PMC7848297, DOI: 10.1002/sctm.20-0014.Peer-Reviewed Original ResearchConceptsDopamine neuronsInduced pluripotent stem cellsParkinson's diseaseStem cell-derived dopamine neuronsPD cell therapyMidbrain dopamine neuronsLong-term survivalTransplant of cellsStem cellsHuman induced pluripotent stem cellsPluripotent stem cellsNeuron preparationsMitomycin C treatmentAthymic ratsDrug selectionUndesirable proliferationCell therapyRobust survivalLower proliferationVivo functionNeuronsTransplantationSurvivalProliferative cellsDisease
2017
Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo
Wakeman D, Hiller B, Marmion D, McMahon C, Corbett G, Mangan K, Ma J, Little L, Xie Z, Perez-Rosello T, Guzman J, Surmeier D, Kordower J. Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. Stem Cell Reports 2017, 9: 149-161. PMID: 28579395, PMCID: PMC5511045, DOI: 10.1016/j.stemcr.2017.04.033.Peer-Reviewed Original ResearchConceptsParkinson's diseaseDopamine neuronsMidbrain dopamine neuronsStem cell therapyGrafted neuronsHost striatumCell-based therapiesPluripotent stem cell therapyFunctional deficitsPrimate modelFiber innervationParkinsonian phenotypeTherapeutic efficacyTransplantation studiesCell therapyNeuronsSignificant reversalTranslational developmentBehavioral assessmentClinical applicationTherapyElectrophysiological signaturesRatsDiseaseMinimal manipulation
2011
Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease
Kriks S, Shim J, Piao J, Ganat Y, Wakeman D, Xie Z, Carrillo-Reid L, Auyeung G, Antonacci C, Buch A, Yang L, Beal M, Surmeier D, Kordower J, Tabar V, Studer L. Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease. Nature 2011, 480: 547-551. PMID: 22056989, PMCID: PMC3245796, DOI: 10.1038/nature10648.Peer-Reviewed Original ResearchCell Transplantation and Gene Therapy in Parkinson's Disease
Wakeman D, Dodiya H, Kordower J. Cell Transplantation and Gene Therapy in Parkinson's Disease. Annals Of Global Health 2011, 78: 126-158. PMID: 21259269, DOI: 10.1002/msj.20233.Peer-Reviewed Original ResearchConceptsParkinson's diseaseLong-term disease modificationFetal dopamine neuronsGraft-induced dyskinesiaStem cell transplantationCourse of diseaseDirect cell replacementProgressive neurodegenerative disorderWidespread clinical useGene therapyPatient-derived cellsDopamine replacementMotor symptomsDisease modificationDopamine deficiencyViral vector deliveryCell transplantationSymptomatic strategiesDopamine neuronsClinical trialsEfficacy profileDopaminergic circuitryMotor neuronsClinical studiesVentral midbrain
2010
Cellular Repair in the Parkinsonian Nonhuman Primate Brain
Redmond DE, Weiss S, Elsworth JD, Roth RH, Wakeman DR, Bjugstad KB, Collier TJ, Blanchard BC, Teng YD, Synder EY, Sladek JR. Cellular Repair in the Parkinsonian Nonhuman Primate Brain. Rejuvenation Research 2010, 13: 188-194. PMID: 20370501, PMCID: PMC2946058, DOI: 10.1089/rej.2009.0960.Peer-Reviewed Original ResearchConceptsHuman neural stem cellsSubstantia nigraDopamine neuronsParkinson's diseaseFetal dopaminergic neuronsFetal striatal tissueReduction of medicationLittle therapeutic benefitParkinsonian motor deficitsSubsequent clinical trialsDopaminergic neural systemsNonhuman primate brainCell replacement strategiesLong-term improvementStem cellsNeural stem cellsMotor deficitsDopaminergic neuronsNeurological signsCellular repairClinical trialsStriatal targetsStriatal tissueTherapeutic benefitParkinson's patients