Featured Publications
Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis
Chaube B, Citrin K, Sahraei M, Singh A, de Urturi D, Ding W, Pierce R, Raaisa R, Cardone R, Kibbey R, Fernández-Hernando C, Suárez Y. Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis. Nature Communications 2023, 14: 8251. PMID: 38086791, PMCID: PMC10716292, DOI: 10.1038/s41467-023-43900-0.Peer-Reviewed Original Research
2020
Cav-1 (Caveolin-1) Deficiency Increases Autophagy in the Endothelium and Attenuates Vascular Inflammation and Atherosclerosis
Zhang X, Ramírez CM, Aryal B, Madrigal-Matute J, Liu X, Diaz A, Torrecilla-Parra M, Suárez Y, Cuervo AM, Sessa WC, Fernández-Hernando C. Cav-1 (Caveolin-1) Deficiency Increases Autophagy in the Endothelium and Attenuates Vascular Inflammation and Atherosclerosis. Arteriosclerosis Thrombosis And Vascular Biology 2020, 40: 1510-1522. PMID: 32349535, PMCID: PMC7253189, DOI: 10.1161/atvbaha.120.314291.Peer-Reviewed Original ResearchConceptsCav-1 deficiencyCav-1-deficient miceCav-1Autophagic fluxCholesterol-rich membrane domainsCav-1 interactsATG5-ATG12 complexEndothelial Cav-1 expressionRegulation of autophagyNovel molecular mechanismExtracellular matrix remodelingAutophagosome componentsMembrane domainsLipid raftsAutophagosome formationPlasma membraneCav-1 expressionMolecular mechanismsLDL transcytosisCellular localizationImportant regulatorAutophagyAutophagy contributesRelevant regulatorMatrix remodeling
2018
MicroRNAs in endothelial cell homeostasis and vascular disease
Fernández-Hernando C, Suárez Y. MicroRNAs in endothelial cell homeostasis and vascular disease. Current Opinion In Hematology 2018, 25: 227-236. PMID: 29547400, PMCID: PMC6175704, DOI: 10.1097/moh.0000000000000424.Peer-Reviewed Original ResearchConceptsVascular diseaseVascular disease preventionPotential therapeutic targetEndothelial cell homeostasisEndothelial cell functionEndothelial dysfunctionEndothelial functionVascular dysfunctionTherapeutic applicationsPotential therapeutic applicationsInvolvement of miRNAsDysregulation of miRNAsEndothelial homeostasisTherapeutic targetDisease preventionDiseaseCell functionRegulatory circuitsCritical modulatorUnanticipated roleTarget genesCell homeostasisDysfunctionMiRNAsHomeostasisNon-coding RNA regulation of endothelial and macrophage functions during atherosclerosis
Aryal B, Suárez Y. Non-coding RNA regulation of endothelial and macrophage functions during atherosclerosis. Vascular Pharmacology 2018, 114: 64-75. PMID: 29551552, PMCID: PMC6177333, DOI: 10.1016/j.vph.2018.03.001.Peer-Reviewed Original ResearchConceptsNon-coding RNAsNon-coding RNA regulationSmall non-coding RNAsMultiple cell functionsRNA regulationMacrophage functionRNA moleculesGene expressionPotential regulatorKey playersVascular biologyPathogenesis of atherosclerosisCell functionSpecific roleLncRNAsRegulationRNAMechanism of actionEndothelial cellsInitial eventVascular integrityRecruitment of monocytesMicroRNAsDevelopment of atherosclerosisBiology
2016
MicroRNAs as regulators of endothelial cell functions in cardiometabolic diseases
Araldi E, Suárez Y. MicroRNAs as regulators of endothelial cell functions in cardiometabolic diseases. Biochimica Et Biophysica Acta 2016, 1861: 2094-2103. PMID: 26825686, PMCID: PMC5039046, DOI: 10.1016/j.bbalip.2016.01.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEndothelial CellsEndothelium, VascularHeart DiseasesHumansLipid MetabolismMetabolic DiseasesMicroRNAsConceptsSmall non-coding RNAsLipid/energy metabolismCarlos Fernández-HernandoRegulation of ECNon-coding RNAsRole of miRNAsEndothelial cellsYajaira SuárezTissue homeostasisCell deathEndothelial cell functionEnvironmental stimuliEnergy metabolismMicroRNAsCell proliferationImportant functionsPotential therapeutic applicationsCell functionMiRNAsDifferent cardiometabolic diseasesMetabolic imbalanceEC dysfunctionTherapeutic applicationsPresent reviewRelated diseases
2013
MicroRNAs as pharmacological targets in endothelial cell function and dysfunction
Chamorro-Jorganes A, Araldi E, Suárez Y. MicroRNAs as pharmacological targets in endothelial cell function and dysfunction. Pharmacological Research 2013, 75: 15-27. PMID: 23603154, PMCID: PMC3752325, DOI: 10.1016/j.phrs.2013.04.002.Peer-Reviewed Original ResearchConceptsEndothelial cell functionShort non-coding RNAsCell functionPost-transcriptional levelNon-coding RNAsEndothelial-specific microRNAsGene expressionMorphogenic capacityCritical regulatorNormal endothelial cell functionMicroRNAsCell dysfunctionEndothelial cell dysfunctionPathophysiological conditionsLatest insightsParacrine mannerPharmacological targetsEndothelial cellsTherapeutic potentialBarrier functionTraffickingRNALeukocyte traffickingRegulatorTarget
2012
miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish
Stahlhut C, Suárez Y, Lu J, Mishima Y, Giraldez AJ. miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish. Development 2012, 139: 4356-4365. PMID: 23132244, PMCID: PMC3509730, DOI: 10.1242/dev.083774.Peer-Reviewed Original ResearchConceptsMiR-1/206Post-transcriptional modulatorsMiRNA-target interactionsMiR-1Appropriate physiological responsesRegulation of VEGFAZebrafish developmentEmbryonic developmentTarget protectorNovel functionPrecise regulationGene expressionMorphogenetic activityDevelopmental angiogenesisPutative targetsRegulate angiogenesisEssential processMiR-206Physiological responsesCellular communicationVEGFA expressionGrowth factorVascular endothelial growth factorExpressionAngiogenesis
2010
Microregulation of Plaque Neovascularization
Suárez Y. Microregulation of Plaque Neovascularization. Arteriosclerosis Thrombosis And Vascular Biology 2010, 30: 1500-1501. PMID: 20631347, DOI: 10.1161/atvbaha.110.209551.Commentaries, Editorials and Letters
2009
Cutting Edge: TNF-Induced MicroRNAs Regulate TNF-Induced Expression of E-Selectin and Intercellular Adhesion Molecule-1 on Human Endothelial Cells: Feedback Control of Inflammation
Suárez Y, Wang C, Manes TD, Pober JS. Cutting Edge: TNF-Induced MicroRNAs Regulate TNF-Induced Expression of E-Selectin and Intercellular Adhesion Molecule-1 on Human Endothelial Cells: Feedback Control of Inflammation. The Journal Of Immunology 2009, 184: 21-25. PMID: 19949084, PMCID: PMC2797568, DOI: 10.4049/jimmunol.0902369.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedE-SelectinEndothelial CellsFeedback, PhysiologicalGene ExpressionGene Expression RegulationHumansImmunohistochemistryInflammationIntercellular Adhesion Molecule-1MicroRNAsOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionTransfectionTumor Necrosis Factor-alphaConceptsEndothelial cellsGene expressionUntranslated regionHuman endothelial cellsMiRNAsCultured endothelial cellsTarget sequenceMicroRNA pairsNegative feedback controlMiR-31Adhesion moleculesCellsExpressionNeutrophil adhesionE-selectinAdhesion molecule-1AdhesionTransfectionIntercellular adhesion molecule-1MRNAMolecule-1SequenceEndothelial adhesion moleculesSpecific antagonismICAM-1MicroRNAs As Novel Regulators of Angiogenesis
Suárez Y, Sessa WC. MicroRNAs As Novel Regulators of Angiogenesis. Circulation Research 2009, 104: 442-454. PMID: 19246688, PMCID: PMC2760389, DOI: 10.1161/circresaha.108.191270.Peer-Reviewed Original ResearchConceptsInvolvement of miRNAsShort noncoding RNAsPosttranscriptional regulationNoncoding RNAsNovel regulatorKey regulatorNegative regulatorGene expressionAspects of developmentNew blood vesselsRegulatorVascular biologyCurrent experimental evidencePotential therapeutic applicationsMiRNAsMicroRNAsAngiogenic processEndothelial cellsRegulationAbnormal angiogenesisTherapeutic applicationsAngiogenesisRNABiologyHomeostasis
2008
Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis
Suárez Y, Fernández-Hernando C, Yu J, Gerber SA, Harrison KD, Pober JS, Iruela-Arispe ML, Merkenschlager M, Sessa WC. Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 14082-14087. PMID: 18779589, PMCID: PMC2544582, DOI: 10.1073/pnas.0804597105.Peer-Reviewed Original ResearchConceptsEndothelial miRNAsPostnatal angiogenesisMiR-17Posttranscriptional gene regulationLoss of DicerGeneration of miRNAsExpression of miRNAsCluster miR-17Cell-specific inactivationGene regulationAngiogenic responseEndothelial microRNAsInduced expressionMiRNAsAspects of developmentEndothelial cell proliferationVariety of stimuliCell proliferationDicerThrombospondin-1Exogenous VEGFMicroRNAsExpressionEndothelial cellsRegulationProhibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Schleicher M, Shepherd BR, Suarez Y, Fernandez-Hernando C, Yu J, Pan Y, Acevedo LM, Shadel GS, Sessa WC. Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. Journal Of Cell Biology 2008, 180: 101-112. PMID: 18195103, PMCID: PMC2213620, DOI: 10.1083/jcb.200706072.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCells, CulturedCellular SenescenceCytoskeletonElectron Transport Complex IEndothelial CellsEndothelium, VascularHumansMiceMice, Inbred StrainsMitochondriaMitochondrial MembranesNeovascularization, PhysiologicNeuropeptidesPhosphatidylinositol 3-KinasesProhibitinsProto-Oncogene Proteins c-aktRac GTP-Binding ProteinsRac1 GTP-Binding ProteinReactive Oxygen SpeciesRepressor ProteinsSignal TransductionConceptsProhibitin 1Mitochondrial functionKnockdown of PHB1Inner mitochondrial membraneEndothelial cell motilityEndothelial cellsCytoskeletal rearrangementsMitochondrial membraneVivo angiogenesis assaysCell motilityAngiogenic capacityCellular senescenceReactive oxygen speciesMitochondrial productionFunctional blood vesselsImportant regulatorSenescenceAngiogenesis assayTube formationOxygen speciesVascular homeostasisCellsVascular systemRac1Yeast
2007
Alloimmunity to Human Endothelial Cells Derived from Cord Blood Progenitors
Suárez Y, Shepherd BR, Rao DA, Pober JS. Alloimmunity to Human Endothelial Cells Derived from Cord Blood Progenitors. The Journal Of Immunology 2007, 179: 7488-7496. PMID: 18025193, DOI: 10.4049/jimmunol.179.11.7488.Peer-Reviewed Original ResearchConceptsCell-derived endothelial cellsEndothelial progenitor cellsCord blood endothelial progenitor cellsBlood endothelial progenitor cellsEndothelial cellsCord blood progenitorsExpression of proteinsMemory T cell responsesHuman endothelial cellsT cell responsesProgenitor cellsBlood progenitorsOrgan repairRegenerative medicineAllogeneic responseAdhesion moleculesTissue engineeringTissue sourcesMHC moleculesCell responsesSuch cellsCellsSame donorAlloimmunityVitroLoss of Akt1 Leads to Severe Atherosclerosis and Occlusive Coronary Artery Disease
Fernández-Hernando C, Ackah E, Yu J, Suárez Y, Murata T, Iwakiri Y, Prendergast J, Miao RQ, Birnbaum MJ, Sessa WC. Loss of Akt1 Leads to Severe Atherosclerosis and Occlusive Coronary Artery Disease. Cell Metabolism 2007, 6: 446-457. PMID: 18054314, PMCID: PMC3621848, DOI: 10.1016/j.cmet.2007.10.007.Peer-Reviewed Original ResearchMeSH KeywordsAcute Coronary SyndromeAnimalsApolipoproteins EApoptosisAtherosclerosisBone Marrow TransplantationCoronary OcclusionDisease Models, AnimalEndothelial CellsFemaleHumansInflammation MediatorsMacrophagesMaleMiceMice, KnockoutNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIProto-Oncogene Proteins c-aktConceptsLoss of Akt1Apolipoprotein E knockout backgroundOcclusive coronary artery diseaseBone marrow transfer experimentsAcute coronary syndromeCoronary artery diseaseLesion expansionCoronary syndromeCoronary atherosclerosisSevere atherosclerosisArtery diseaseInflammatory mediatorsCoronary lesionsVascular protectionVascular originProinflammatory genesENOS phosphorylationCardiovascular systemLesion formationGenetic ablationEndothelial cellsAtherogenesisEnhanced expressionKnockout backgroundVessel wallDicer Dependent MicroRNAs Regulate Gene Expression and Functions in Human Endothelial Cells
Suárez Y, Fernández-Hernando C, Pober JS, Sessa WC. Dicer Dependent MicroRNAs Regulate Gene Expression and Functions in Human Endothelial Cells. Circulation Research 2007, 100: 1164-1173. PMID: 17379831, DOI: 10.1161/01.res.0000265065.26744.17.Peer-Reviewed Original ResearchConceptsGene expressionHuman endothelial cellsEndogenous miRNA levelsImportance of miRNAsMaturation of microRNAsEC gene expressionEndothelial cellsTek/TieKnockdown of DicerDICER-dependent microRNAsRole of DicerMiRNA expression profilesKDR/VEGFR2MiR-222/221Dicer knockdownDependent microRNAsSynthase protein levelsDicerKey regulatorExpression profilesKey enzymePhysiological pathwaysCord formationEndothelial biologyMiRNAs
2006
Vascularization and engraftment of a human skin substitute using circulating progenitor cell‐derived endothelial cells
Shepherd BR, Enis DR, Wang F, Suarez Y, Pober JS, Schechner JS, Shepherd B, Enis D, Wang F, Suarez Y, Pober J, Scheduier J. Vascularization and engraftment of a human skin substitute using circulating progenitor cell‐derived endothelial cells. The FASEB Journal 2006, 20: 1739-1741. PMID: 16807367, DOI: 10.1096/fj.05-5682fje.Peer-Reviewed Original Research