2024
An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus
Sagawa C, Thomson G, Mermaz B, Vernon C, Liu S, Jacob Y, Irish V. An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus. Plant Methods 2024, 20: 148. PMID: 39342225, PMCID: PMC11438372, DOI: 10.1186/s13007-024-01274-4.Peer-Reviewed Original ResearchSimultaneous editing of multiple genesMultiple genesGene editingEudicot plant speciesPol III promotersTarget multiple genesGenus fallRPS5A promoterCRISPR/Cas9 gene editingCRISPR/Cas9-mediated gene editingMultiplex gene editingGenome engineeringIII promotersGenetic screeningPlant speciesCas9 endonucleaseEditing efficiencyGene editing efficiencySgRNAGenesArabidopsisUBQ10SpeciesSimultaneous editingPromoter
2017
Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress
LeBlanc C, Zhang F, Mendez J, Lozano Y, Chatpar K, Irish V, Jacob Y. Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress. The Plant Journal 2017, 93: 377-386. PMID: 29161464, DOI: 10.1111/tpj.13782.Peer-Reviewed Original ResearchConceptsCRISPR/Green fluorescent protein (GFP) reporter geneCRISPR/Cas9 systemFluorescent protein reporter geneCRISPR/Cas9Off-target mutationsArabidopsis plantsEukaryotic genomesDifferent organismsSomatic tissuesCitrus plantsCas9 systemDNA breaksReporter geneTarget mutagenesisTargeted mutationsMutation rateMutagenesisImportance of temperatureArabidopsisHeat stressPlantsMutationsQuantitative assayEukaryotes
2010
The Arabidopsis Floral Homeotic Proteins APETALA3 and PISTILLATA Negatively Regulate the BANQUO Genes Implicated in Light Signaling
Mara CD, Huang T, Irish VF. The Arabidopsis Floral Homeotic Proteins APETALA3 and PISTILLATA Negatively Regulate the BANQUO Genes Implicated in Light Signaling. The Plant Cell 2010, 22: 690-702. PMID: 20305124, PMCID: PMC2861465, DOI: 10.1105/tpc.109.065946.Peer-Reviewed Original ResearchConceptsPetal identityBHLH transcription factorsDevelopmental signaling pathwaysSecond whorl organsBHLH proteinsLight signalingHelix proteinsAPETALA3Light responseTranscription factorsGene productsPistillataChlorophyll levelsSignaling pathwaysGenesRegulatory processesProteinAppropriate regulationHFR1ArabidopsisPhotomorphogenesisMutantsSepalsCarpelsPhytochrome
2006
Functional Analyses of Two Tomato APETALA3 Genes Demonstrate Diversification in Their Roles in Regulating Floral Development
de Martino G, Pan I, Emmanuel E, Levy A, Irish VF. Functional Analyses of Two Tomato APETALA3 Genes Demonstrate Diversification in Their Roles in Regulating Floral Development. The Plant Cell 2006, 18: 1833-1845. PMID: 16844904, PMCID: PMC1533988, DOI: 10.1105/tpc.106.042978.Peer-Reviewed Original ResearchConceptsCore eudicotsFloral developmentMADS-box transcription factorsDifferent expression domainsBox transcription factorFunctional differencesAP3 lineageAPETALA3 (AP3) geneEuAP3 lineageStamen identityAncestral roleStamen developmentHomeotic transformationsLineage genesExpression domainsBiochemical capabilitiesTranscription factorsFunctional analysisFunction mutationsGenesLineagesArabidopsisEudicotsEquivalent domainsExpression contributesDuplication, Diversification, and Comparative Genetics of Angiosperm MADS‐Box Genes
Irish V. Duplication, Diversification, and Comparative Genetics of Angiosperm MADS‐Box Genes. Advances In Botanical Research 2006, 44: 129-161. DOI: 10.1016/s0065-2296(06)44003-9.Peer-Reviewed Original ResearchMADS-box genesFunctional analysisVirus-induced geneConsiderable morphological diversityNonmodel speciesProtein evolutionComparative geneticsInformative taxaAngiosperm flowersPhylogenetic contextFlower developmentRegulatory evolutionModel speciesPhenotypic variationFloral morphologyMorphological diversityDevelopmental basisGenesDuplicationDiversificationSpeciesDiversityArabidopsisDevelopmental shiftSuch hypotheses
2002
Response: Missing links: the genetic architecture of flower and floral diversification
Baum D, Doebley J, Irish V, Kramer E. Response: Missing links: the genetic architecture of flower and floral diversification. Trends In Plant Science 2002, 7: 31-34. DOI: 10.1016/s1360-1385(01)02181-1.Peer-Reviewed Original ResearchGenomic approachesComplementary genetic approachesFlower evolutionFloral diversificationGene phylogenyFlower developmentGene familyGenetic architectureEST databaseGenetic approachesImportant genesExtraordinary diversityExpression dataFloral differentiationGenesModel systemFlowersSpeciesSite of actionArabidopsisPhylogenyTaxaDiversityDiversificationDifferentiation
2000
Variations on a theme: flower development and evolution
Irish V. Variations on a theme: flower development and evolution. Genome Biology 2000, 1: reviews1015.1. PMID: 11178237, PMCID: PMC138852, DOI: 10.1186/gb-2000-1-2-reviews1015.Peer-Reviewed Original Research
1999
CYP78A5 encodes a cytochrome P450 that marks the shoot apical meristem boundary in Arabidopsis
Zondlo S, Irish V. CYP78A5 encodes a cytochrome P450 that marks the shoot apical meristem boundary in Arabidopsis. The Plant Journal 1999, 19: 259-268. PMID: 10476073, DOI: 10.1046/j.1365-313x.1999.00523.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceArabidopsisBase SequenceCloning, MolecularCytochrome P-450 Enzyme SystemDNA, PlantGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantGenes, PlantIn Situ HybridizationMeristemMicroscopy, Electron, ScanningMolecular Sequence DataMutationPhenotypePlants, Genetically ModifiedConceptsShoot apical meristemApical meristemMeristem functionFloral developmentReproductive shoot apical meristemPutative cytochrome P450 monooxygenaseCytochrome P450 monooxygenaseDifferentiation of cellsSHOOT MERISTEMLESSMultiple cell typesMutant backgroundOrgan primordiaCYP78A5Shoot structureMeristematic zoneP450 monooxygenaseMeristemGenesCell typesNormal developmentArabidopsisFirst memberCytochrome P450ExpressionDynamic patterns
1998
Floral development in Arabidopsis
Irish V. Floral development in Arabidopsis. Plant Physiology And Biochemistry 1998, 36: 61-68. DOI: 10.1016/s0981-9428(98)80091-0.Peer-Reviewed Original ResearchFloral homeotic genesArabidopsis flowersHomeotic genesFloral meristem identity genesMeristem identity genesClasses of genesDifferent organ typesMeristematic cell populationOrgan identityIdentity genesFloral developmentMutational analysisMeristemGenesOrgan typeMolecular analysisTissue typesCell populationsFlowersComplex arrayDiscrete regionsConcentric whorlsArabidopsisSepalsCarpels
1992
A fate map of the Arabidopsis embryonic shoot apical meristem
Irish V, Sussex I. A fate map of the Arabidopsis embryonic shoot apical meristem. Development 1992, 115: 745-753. DOI: 10.1242/dev.115.3.745.Peer-Reviewed Original Research