Saúl Rojas Sánchez, PhD
Associate Research ScientistCards
About
Research
Publications
2025
Deep mutational scanning of the Trypanosoma brucei developmental regulator RBP6 reveals an essential disordered region influenced by positive residues
Rojas-Sánchez S, Kolev N, Tschudi C. Deep mutational scanning of the Trypanosoma brucei developmental regulator RBP6 reveals an essential disordered region influenced by positive residues. Nature Communications 2025, 16: 1168. PMID: 39885181, PMCID: PMC11782513, DOI: 10.1038/s41467-025-56553-y.Peer-Reviewed Original ResearchConceptsN-terminal intrinsically disordered regionRNA binding protein 6RNA recognition motifDeep mutational scanningMutational scanningDisordered regionsProtein-RNA interactionsIntrinsically disordered regionsComplex developmental programPositively charged residuesSingle-point variantsProtein-RNARNA recognitionDeleterious mutationsMutational constraintsProcyclic formsAnimal African trypanosomiasisTrypanosoma bruceiCharged residuesPrimary structureRegained infectivityDevelopmental programRegulatory roleProtein 6Positive residues
2021
Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma brucei
Toh JY, Nkouawa A, Sánchez SR, Shi H, Kolev NG, Tschudi C. Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma brucei. Scientific Reports 2021, 11: 5755. PMID: 33707699, PMCID: PMC7952579, DOI: 10.1038/s41598-021-85225-2.Peer-Reviewed Original ResearchConceptsGene expression regulatory networksMetacyclic variant surface glycoproteinSingle RNA-binding proteinExpression regulatory networksRNA-binding proteinTsetse fly vectorBloodstream form trypanosomesAlanine-rich proteinPutative nucleic acidRNA-seq dataSubset of transcriptsCell linesDevelopmental progressionVariant surface glycoproteinMitochondrial genomeOverexpression cell linesFunction phenotypesRegulatory networksProcyclic trypanosomesTrypanosoma bruceiNegative regulatorRich proteinImportant humanMetacyclic parasitesGenes
2016
Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major
MORENO-CAMPOS R, FLORENCIO-MARTÍNEZ L, NEPOMUCENO-MEJÍA T, ROJAS-SÁNCHEZ S, VÉLEZ-RAMÍREZ D, PADILLA-MEJÍA N, FIGUEROA-ANGULO E, MANNING-CELA R, MARTÍNEZ-CALVILLO S. Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major. Parasitology 2016, 143: 1917-1929. PMID: 27707420, DOI: 10.1017/s0031182016001712.Peer-Reviewed Original ResearchTranscription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene
Rojas-Sánchez S, Figueroa-Angulo E, Moreno-Campos R, Florencio-Martínez L, Manning-Cela R, Martínez-Calvillo S. Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene. Parasites & Vectors 2016, 9: 401. PMID: 27430335, PMCID: PMC4950102, DOI: 10.1186/s13071-016-1682-3.Peer-Reviewed Original Research
2015
BRF1, a subunit of RNA polymerase III transcription factor TFIIIB, is essential for cell growth of Trypanosoma brucei
VÉLEZ-RAMÍREZ D, FLORENCIO-MARTÍNEZ L, ROMERO-MEZA G, ROJAS-SÁNCHEZ S, MORENO-CAMPOS R, ARROYO R, ORTEGA-LÓPEZ J, MANNING-CELA R, MARTÍNEZ-CALVILLO S. BRF1, a subunit of RNA polymerase III transcription factor TFIIIB, is essential for cell growth of Trypanosoma brucei. Parasitology 2015, 142: 1563-1573. PMID: 26337955, DOI: 10.1017/s0031182015001122.Peer-Reviewed Original Research