SF3B1 Mutations Lead to Changes in Fine-Scale Chromatin Organization through Impaired Transcription
Roy R, Gupta A, Pillai M, Boddu P. SF3B1 Mutations Lead to Changes in Fine-Scale Chromatin Organization through Impaired Transcription. Blood 2024, 144: 1813. DOI: 10.1182/blood-2024-211548.Peer-Reviewed Original ResearchPol II densityPol IIGenome binsGenome organizationMutant cellsSplicing factorsEnhancer-promoterEP pairsH3K27ac ChIP-seq dataHi-C protocolPol II distributionChIP-seq dataChIP-seq datasetsEnhancer-promoter interactionsPol II transcriptionRNA polymerase IIPromoter chromatin architecturePol II pausingSF3B1-mutant cellsPaused Pol IIChromatin organization changesFine-scalePutative enhancer regionsGene promoter regionChIP-seqTranscription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape
Boddu P, Gupta A, Roy R, De La Peña Avalos B, Olazabal-Herrero A, Neuenkirchen N, Zimmer J, Chandhok N, King D, Nannya Y, Ogawa S, Lin H, Simon M, Dray E, Kupfer G, Verma A, Neugebauer K, Pillai M. Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape. Molecular Cell 2024, 84: 1475-1495.e18. PMID: 38521065, PMCID: PMC11061666, DOI: 10.1016/j.molcel.2024.02.032.Peer-Reviewed Original ResearchRate of RNA polymerase IIChromatin landscapeElongation defectsElongation rate of RNA polymerase IIImpaired protein-protein interactionsSplicing of pre-messenger RNATranscription elongation defectsRNA polymerase IIProtein-protein interactionsPre-messenger RNACancer-associated mutationsIsogenic cell linesSin3/HDAC complexGene bodiesPolymerase IIChromatin accessibilityH3K4me3 markChromatin changesMutant SF3B1ChromatinMutant mouse modelsEpigenetic disordersEpigenetic factorsHuman diseasesMutant state