2024
Tet2 Loss in Hematopoietic Stem Cells Triggers Chromatin Reorganization through DNA Methylation Shifts
Roy R, Pillai M, Boddu P. Tet2 Loss in Hematopoietic Stem Cells Triggers Chromatin Reorganization through DNA Methylation Shifts. Blood 2024, 144: 1812. DOI: 10.1182/blood-2024-211494.Peer-Reviewed Original ResearchTopologically associating domainsDisruption of TAD boundariesTopologically associating domains boundariesTAD boundariesMutant cellsChromatin organizationChromatin compartmentsDNA methylationLT-HSCsKO cellsEnhancer-promoterHigher-order chromatin organizationTet2 lossStudy of chromatin organizationGene expressionMethyl-seq dataHypermethylated differentially methylated regionsPaired-end readsWT cellsGene regulatory networksConversion of 5-methylcytosineDifferentially methylated regionsMyelodysplastic syndromeCompartment shiftsChanges to DNA methylation
2023
Transcription Defects in SF3B1K700E Induce Targetable Alterations in the Chromatin Landscape
Boddu P, Gupta A, Roy R, Herrero A, Verma A, Neugebauer K, Pillai M. Transcription Defects in SF3B1K700E Induce Targetable Alterations in the Chromatin Landscape. Blood 2023, 142: 709. DOI: 10.1182/blood-2023-188083.Peer-Reviewed Original ResearchChromatin organizationSuch epigenetic changesGenome editing approachesRNA splicing factorsChromatin landscapeSingle mutant alleleEpigenetic landscapeGenomic integrityTranscription defectTranscription kineticsSplicing factorsChIP-seqEpigenetic regulatorsEpigenetic changesEpigenetic therapyMutant allelesEditing approachesFactor mutationsK562 cell lineDownstream effectsCell linesMyeloid disordersClonal myeloid disordersHDAC pathwayMutations