2014
Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method
Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method. Molecular Biology Of The Cell 2014, 25: 3501-3514. PMID: 25143395, PMCID: PMC4230612, DOI: 10.1091/mbc.e13-01-0004.Peer-Reviewed Original ResearchConceptsFission yeastEndocytic actin patchesWild-type cellsEndocytic patchesActin patchesQuantitative microscopyActin assemblyCellular processesVesicle movementEndocytic vesiclesInterphase cellsVesicle formationMolecular mechanismsPatch dynamicsYeastCell lengthGlobal analysisNumber of patchesMicroscopy moviesCellsClathrinEndocytosisNew toolValuable toolPatches
2013
Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches
Chen Q, Pollard TD. Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches. Current Biology 2013, 23: 1154-1162. PMID: 23727096, PMCID: PMC4131202, DOI: 10.1016/j.cub.2013.05.005.Peer-Reviewed Original ResearchConceptsSites of endocytosisActin patchesWild-type cellsArp2/3 complexMother filamentActin filamentsAdaptor proteinActin polymerizationYeast cellsBranched actin filament networksEndocytic adaptor proteinActivators of Arp2/3Fission yeast cellsActin filament severingActin filament networkNew patchesEndocytic sitesQuantitative fluorescence microscopyMutant cofilinsMutant cellsNascent sitesFilament severingCofilinFilament networkEndocytosis
2011
Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast
Arasada R, Pollard TD. Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast. Current Biology 2011, 21: 1450-1459. PMID: 21885283, PMCID: PMC3350781, DOI: 10.1016/j.cub.2011.07.046.Peer-Reviewed Original ResearchConceptsF-BAR proteinsNucleation-promoting factorsActin patchesFission yeastArp2/3 complexActin polymerizationActin filamentsGenetic interaction experimentsSites of endocytosisPeripheral membrane proteinsActin binding proteinsClathrin-mediated endocytosisMembrane scissionYeast showSH3 domainCdc15pMembrane proteinsMembrane tubulesPlasma membraneGenetic analysisBinding proteinDistinct rolesEndocytosisProteinClathrin