2019
Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides
Chou SZ, Pollard TD. Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 4265-4274. PMID: 30760599, PMCID: PMC6410863, DOI: 10.1073/pnas.1807028115.Peer-Reviewed Original ResearchConceptsATP hydrolysisActin filamentsBarbed endsMultiple favorable interactionsCryo-electron microscopyNetwork of interactionsShort-pitch helixActin polymerizationC-terminusAdjacent subunitsSubdomain 2Conformational changesEM structuresBinding loopSubdomain 3SubunitsPhosphate dissociationPointed endRelease sitesFilamentsActive siteConformationADPBackbone conformationSide chains
2013
Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches
Chen Q, Pollard TD. Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches. Current Biology 2013, 23: 1154-1162. PMID: 23727096, PMCID: PMC4131202, DOI: 10.1016/j.cub.2013.05.005.Peer-Reviewed Original ResearchConceptsSites of endocytosisActin patchesWild-type cellsArp2/3 complexMother filamentActin filamentsAdaptor proteinActin polymerizationYeast cellsBranched actin filament networksEndocytic adaptor proteinActivators of Arp2/3Fission yeast cellsActin filament severingActin filament networkNew patchesEndocytic sitesQuantitative fluorescence microscopyMutant cofilinsMutant cellsNascent sitesFilament severingCofilinFilament networkEndocytosis
2011
Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast
Arasada R, Pollard TD. Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast. Current Biology 2011, 21: 1450-1459. PMID: 21885283, PMCID: PMC3350781, DOI: 10.1016/j.cub.2011.07.046.Peer-Reviewed Original ResearchConceptsF-BAR proteinsNucleation-promoting factorsActin patchesFission yeastArp2/3 complexActin polymerizationActin filamentsGenetic interaction experimentsSites of endocytosisPeripheral membrane proteinsActin binding proteinsClathrin-mediated endocytosisMembrane scissionYeast showSH3 domainCdc15pMembrane proteinsMembrane tubulesPlasma membraneGenetic analysisBinding proteinDistinct rolesEndocytosisProteinClathrin