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Phospholipase PLCγ1 promotes phase separation in T cells
Phospholipase Cγ1 (PLCγ1) hydrolyzes PIP2 to generate IP3 and DAG to transduce T cell receptor (TCR) signaling. We revealed a lipase-independent function of PLCγ1 in promoting phase separation of T cell signaling components. We showed that PLCγ1crosslinks LAT, a key adaptor protein in forming signaling condensates in the TCR pathway. PLCγ1 also protects LAT from CD45-mediated dephosphorylation and promotes downstream ERK activation and actin polymerization. Interestingly, PLCγ1 promotes LAT condensation only at low concentrations but not high concentrations. This could be explained by a change in internal organization of the condensates using a coarse-grained model-based simulation.
Source: Journal of Cell Biology